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Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification.

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اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: San Francisco, CA : Public Library of Science
    • الموضوع:
      Chlamydia Infections/*diagnosis ; Chlamydia trachomatis/*genetics ; Gonorrhea/*diagnosis ; Neisseria gonorrhoeae/*genetics ; Recombinases/*genetics; Chlamydia Infections/microbiology ; DNA, Bacterial/genetics ; Female ; Gonorrhea/microbiology ; Humans ; Pregnancy ; Pregnant Women ; Real-Time Polymerase Chain Reaction/methods ; Sensitivity and Specificity ; Sexually Transmitted Diseases/genetics ; Sexually Transmitted Diseases/microbiology
    • نبذة مختصرة :
      Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are the main pathogenic microorganisms causing sexually transmitted infections. In this study, a multiplex thermostable recombinase polymerase amplification-lateral flow detection (RPA-LFD) assay was established, and the reaction conditions such as the ratio of primer concentration, magnesium ion concentration, amplification time and template DNA concentration in the multiplex RPA reaction were optimized. The optimized multiplex RPA-LFD method was used to detect both CT and NG positive control plasmids, and it was found that the LFD could be used to obtain visible results when the plasmid copy number was only 200. The sensitivity of the multiplex RPA-LFD method used for clinical samples was 85.62 (95% CI at 53.66-97.29) for NG detection and 90.90 (95% CI at 57.12-99.52) for CT detection.
      Competing Interests: The authors have declared that no competing interests exist.
    • Comments:
      Erratum in: PLoS One. 2022 Jul 15;17(7):e0271836. (PMID: 35839235)
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    • الرقم المعرف:
      0 (DNA, Bacterial)
      0 (Recombinases)
    • الموضوع:
      Date Created: 20210504 Date Completed: 20211019 Latest Revision: 20220715
    • الموضوع:
      20240829
    • الرقم المعرف:
      PMC8096098
    • الرقم المعرف:
      10.1371/journal.pone.0251119
    • الرقم المعرف:
      33945580