Formation of Proto-Kranz in C3 Rice Induced by Spike-Stalk Injection Method.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Photosynthesis*; Oryza/*physiology ; Plant Leaves/*physiology ; Plant Proteins/*metabolism ; Plants, Genetically Modified/*physiology ; Sorghum/*physiology; Carbon Dioxide/metabolism ; Genome, Plant ; Oryza/anatomy & histology ; Oryza/genetics ; Plant Leaves/anatomy & histology ; Plant Leaves/genetics ; Plant Proteins/genetics ; Plants, Genetically Modified/anatomy & histology ; Plants, Genetically Modified/genetics ; Sorghum/anatomy & histology ; Sorghum/genetics

Introduction of C4 photosynthetic traits into C3 crops is an important strategy for improving photosynthetic capacity and productivity. Here, we report the research results of a variant line of sorghum-rice (SR) plant with big panicle and high spikelet density by introducing sorghum genome DNA into rice by spike-stalk injection. The whole-genome resequencing showed that a few sorghum genes could be integrated into the rice genome. Gene expression was confirmed for two C4 photosynthetic enzymes containing pyruvate, orthophosphate dikinase and phosphoenolpyruvate carboxykinase. Exogenous sorghum DNA integration induced a series of key traits associated with the C4 pathway called "proto-Kranz" anatomy, including leaf thickness, bundle sheath number and size, and chloroplast size in bundle sheath cells. Significantly, transgenic plants exhibited enhanced photosynthetic capacity resulting from both photosynthetic CO 2 -concentrating effect and improved energy balance, which led to an increase in carbohydrate levels and productivity. Furthermore, such rice plant exhibited delayed leaf senescence. In summary, this study provides a proof for the feasibility of inducing the transition from C3 leaf anatomy to proto-Kranz by spike-stalk injection to achieve efficient photosynthesis and increase productivity.

Retraction in: Int J Mol Sci. 2021 Nov 03;22(21):. (PMID: 34769522)

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CX173022 Jiangsu Agricultural Science and Technology Innovation Fund; BK20171034 Natural Science Foundation of Jiangsu Province

Keywords: C4 rice; crop improvement; photosynthetic efficiency; proto-Kranz; spike-stalk injection

0 (Plant Proteins)
142M471B3J (Carbon Dioxide)

Date Created: 20210430 Date Completed: 20210513 Latest Revision: 20211113

20240628

PMC8122280

10.3390/ijms22094305

33919137