A promising dual-drug targeted delivery system in cancer therapy: nanocomplexes of folate-apoferritin-conjugated cationic solid lipid nanoparticles.

Publisher: Informa Healthcare Country of Publication: England NLM ID: 9610932 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-9867 (Electronic) Linking ISSN: 10837450 NLM ISO Abbreviation: Pharm Dev Technol Subsets: MEDLINE

Publication: London : Informa Healthcare
Original Publication: Monticello, NY : Marcel Dekker, c1996-

Neoplasms*/drug therapy ; Neoplasms*/metabolism; Antineoplastic Agents/*chemistry ; Apoferritins/*chemistry ; Doxorubicin/*analogs & derivatives ; Drug Delivery Systems/*methods ; Folic Acid/*chemistry ; Liposomes/*chemistry ; Nanoparticles/*chemistry; Antineoplastic Agents/administration & dosage ; Antineoplastic Agents/metabolism ; Apoferritins/administration & dosage ; Apoferritins/metabolism ; Cations ; Cell Survival/drug effects ; Cell Survival/physiology ; Dose-Response Relationship, Drug ; Doxorubicin/administration & dosage ; Doxorubicin/chemistry ; Doxorubicin/metabolism ; Folic Acid/administration & dosage ; Folic Acid/metabolism ; Humans ; Liposomes/administration & dosage ; Liposomes/metabolism ; MCF-7 Cells ; Nanoparticles/administration & dosage ; Nanoparticles/metabolism

Various nano-sized protein and lipid complexes are being investigated as drug delivery systems. The encapsulation of more than one drug in a single nanocomplex carrier could enhance the therapeutic potency and afford synergistic therapeutic effects. In this study, we developed a novel protein-lipid nanocomplex as a controlled drug delivery system for two important cancer drugs, doxorubicin (DOX) and mitoxantrone (MTO). Apoferritin (AFr) functionalized with folic acid (FA) was used to encapsulate DOX to create the targeted protein nanocomplexes (TPNs). The second drug, MTO, was loaded into the cationic solid lipid nanoparticles (cSLN) to form the liposomal drug nanocomplex particles (MTO-cSLNs). Two complexes were then assembled by tight coupling through ionic interactions to obtain the final drug delivery system, the dual-targeted protein-lipid nanocomplexes (DTPLNs). UV-Vis and fluorescence spectroscopy were used for structural characterization of TPNs and DTPLNs. Transmission electron microscopy (TEM) was used for comprehensive analysis of the final DTPLNs. We confirmed that the DTPLNs display desired time-dependent and pH-dependent drug release behaviors. We also demonstrated the improved anti-cancer efficacy of DOX and MTO in their encapsulated DTPLNs as compared to their free forms. Our results provide promising prospects for the application of the DTPLNs as efficient drug delivery systems.

Keywords: Apoferritin; cationic solid lipid nanoparticles; doxorubicin; dual targeting; mitoxantrone; pH responsive

0 (Antineoplastic Agents)
0 (Cations)
0 (Lipid Nanoparticles)
0 (Liposomes)
0 (apoferritin doxorubicin)
80168379AG (Doxorubicin)
9013-31-4 (Apoferritins)
935E97BOY8 (Folic Acid)

Date Created: 20210426 Date Completed: 20211203 Latest Revision: 20211214

20221213

10.1080/10837450.2021.1920037

33896342