Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Changes in the phosphorylation of nucleotide metabolism‑associated proteins by leukemia inhibitory factor in mouse embryonic stem cells.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: D. A. Spandidos Country of Publication: Greece NLM ID: 101475259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1791-3004 (Electronic) Linking ISSN: 17912997 NLM ISO Abbreviation: Mol Med Rep Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Athens, Greece : D. A. Spandidos
    • الموضوع:
      Leukemia Inhibitory Factor/*metabolism ; Mouse Embryonic Stem Cells/*metabolism ; Nucleotides/*metabolism; Animals ; Cell Line ; Interleukin-6/metabolism ; Janus Kinases/metabolism ; Mice ; Phosphorylation ; Protein Binding ; Proteomics ; Receptors, OSM-LIF/metabolism
    • نبذة مختصرة :
      Leukemia inhibitory factor (LIF) is a stem cell growth factor that maintains self‑renewal of mouse embryonic stem cells (mESCs). LIF is a cytokine in the interleukin‑6 family and signals via the common receptor subunit gp130 and ligand‑specific LIF receptor. LIF causes heterodimerization of the LIF receptor and gp130, activating the Janus kinase/STAT and MAPK pathways, resulting in changes in protein phosphorylation. The present study profiled LIF‑mediated protein phosphorylation changes in mESCs via proteomic analysis. mESCs treated in the presence or absence of LIF were analyzed via two‑dimensional differential in‑gel electrophoresis and protein and phosphoprotein staining. Protein identification was performed by matrix‑assisted laser desorption/ionization‑time of flight mass spectrophotometry. Increased phosphorylation of 16 proteins and decreased phosphorylation of 34 proteins in response to LIF treatment was detected. Gene Ontology terms enriched in these proteins included 'organonitrogen compound metabolic process', 'regulation of mRNA splicing via spliceosome' and 'nucleotide metabolic process'. The present results revealed that LIF modulated phosphorylation levels of nucleotide metabolism‑associated proteins, thus providing insight into the mechanism underlying LIF action in mESCs.
    • References:
      In Vitro Cell Dev Biol Anim. 2003 Nov-Dec;39(10):449-53. (PMID: 14705957)
      Science. 1998 Nov 6;282(5391):1145-7. (PMID: 9804556)
      J Biol Chem. 1996 Nov 22;271(47):30136-43. (PMID: 8939963)
      Genes Dev. 1998 Jul 1;12(13):2048-60. (PMID: 9649508)
      Cell. 1993 Dec 17;75(6):1215-25. (PMID: 8261515)
      Mol Cell Biol. 1998 Nov;18(11):6416-22. (PMID: 9774657)
      J Biol Chem. 2003 Mar 7;278(10):8846-52. (PMID: 12509435)
      BMC Bioinformatics. 2017 Mar 20;18(1):177. (PMID: 28320317)
      Nucleic Acids Res. 2002 May 1;30(9):1959-66. (PMID: 11972333)
      Proc Natl Acad Sci U S A. 1981 Dec;78(12):7634-8. (PMID: 6950406)
      J Biol Chem. 2011 Dec 9;286(49):42690-42703. (PMID: 21969378)
      Science. 2007 Dec 21;318(5858):1917-20. (PMID: 18029452)
      J Biol Chem. 1980 May 25;255(10):4808-13. (PMID: 6102991)
      Cancer Res. 1977 Feb;37(2):535-40. (PMID: 264412)
      J Biol Chem. 2006 Jun 16;281(24):16536-45. (PMID: 16608843)
      Curr Protoc Mol Biol. 2012 Jan;Chapter 23:Unit-23.15. (PMID: 22237859)
      J Biol Chem. 2005 Jan 28;280(4):2904-11. (PMID: 15533935)
      Genome Biol. 2018 Sep 14;19(1):133. (PMID: 30217220)
      Sci Adv. 2019 Nov 27;5(11):eaax7525. (PMID: 31807705)
      Nat Struct Mol Biol. 2013 Sep;20(9):1122-30. (PMID: 23912277)
      Nat Protoc. 2009;4(1):44-57. (PMID: 19131956)
      Curr Biol. 1998 Jan 29;8(3):145-55. (PMID: 9443911)
      RNA Biol. 2018 Feb 1;15(2):251-260. (PMID: 29168431)
      Mol Reprod Dev. 2008 Apr;75(4):614-22. (PMID: 17886269)
      Elife. 2018 May 09;7:. (PMID: 29741478)
      Oncotarget. 2018 Apr 6;9(26):17980-17993. (PMID: 29719584)
      Aging (Albany NY). 2010 Nov;2(11):815-22. (PMID: 21076177)
      J Biol Chem. 2000 Dec 29;275(52):40742-8. (PMID: 11016927)
      Cell. 2007 Nov 30;131(5):861-72. (PMID: 18035408)
      World J Stem Cells. 2015 Jan 26;7(1):1-10. (PMID: 25621101)
      BMB Rep. 2018 May;51(5):242-248. (PMID: 29335068)
      Mol Cell Biol. 2009 Apr;29(8):2092-104. (PMID: 19223464)
      Bioinformatics. 2015 Sep 1;31(17):2912-4. (PMID: 25964631)
      J Biol Chem. 2011 Dec 16;286(50):43370-82. (PMID: 22013067)
      J Biol Chem. 2017 Dec 8;292(49):19952-19958. (PMID: 29084849)
    • Contributed Indexing:
      Keywords: embryonic stem cells; leukemia inhibitory factor; phosphorylation; mouse
    • الرقم المعرف:
      0 (Interleukin-6)
      0 (Leukemia Inhibitory Factor)
      0 (Lif protein, mouse)
      0 (Nucleotides)
      0 (Receptors, OSM-LIF)
      EC 2.7.10.2 (Janus Kinases)
    • الموضوع:
      Date Created: 20210413 Date Completed: 20211018 Latest Revision: 20211018
    • الموضوع:
      20221213
    • الرقم المعرف:
      PMC8060798
    • الرقم المعرف:
      10.3892/mmr.2021.12070
    • الرقم المعرف:
      33846773