Item request has been placed!
×
Item request cannot be made.
×
![loading](/sites/all/modules/hf_eds/images/loading.gif)
Cellular engineering of plant cells for improved therapeutic protein production.
Item request has been placed!
×
Item request cannot be made.
×
![loading](/sites/all/modules/hf_eds/images/loading.gif)
- المؤلفون: Karki U;Karki U;Karki U; Fang H; Fang H; Fang H; Guo W; Guo W; Unnold-Cofre C; Unnold-Cofre C; Xu J; Xu J; Xu J
- المصدر:
Plant cell reports [Plant Cell Rep] 2021 Jul; Vol. 40 (7), pp. 1087-1099. Date of Electronic Publication: 2021 Apr 10.- نوع النشر :
Journal Article; Review- اللغة:
English - المصدر:
- معلومة اضافية
- المصدر: Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-203X (Electronic) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: MEDLINE
- بيانات النشر: Original Publication: Berlin ; New York : Springer, 1981-
- الموضوع: Molecular Biology/*methods ; Plant Cells/*physiology ; Recombinant Proteins/*genetics; Cell Line ; Cells, Cultured ; Gene Editing ; Glycosylation ; Humans ; Plants, Genetically Modified ; Protein Engineering/methods ; RNA Interference ; Recombinant Proteins/metabolism ; Recombinant Proteins/pharmacology
- نبذة مختصرة : In vitro cultured plant cells, in particular the tobacco BY-2 cell, have demonstrated their potential to provide a promising bioproduction platform for therapeutic proteins by integrating the merits of whole-plant cultivation systems with those of microbial and mammalian cell cultures. Over the past three decades, substantial progress has been made in improving the plant cell culture system, resulting in a few commercial success cases, such as taliglucerase alfa (Elelyso ® ), the first FDA-approved recombinant pharmaceutical protein derived from plant cells. However, compared to the major expression hosts (bacteria, yeast, and mammalian cells), plant cells are still largely underutilized, mainly due to low productivity and non-human glycosylation. Modern molecular biology tools, in particular RNAi and the latest genome editing technology CRISPR/Cas9, have been used to modulate the genome of plant cells to create new cell lines that exhibit desired "traits" for producing therapeutic proteins. This review highlights the recent advances in cellular engineering of plant cells towards improved recombinant protein production, including creating cell lines with deficient protease levels or humanized glycosylation, and considers potential development in the future.
- References: Akashi H, Miyagishi M, Taira K (2004) RNAi expression vectors in plant cells. Methods Mol Biol 252:533–543. (PMID: 15017079)
Almon E, Khoury T, Drori A, Gingis-Velitski S, Alon S, Chertkoff R, Mushkat M, Shaaltiel Y, Ilan Y (2017) An oral administration of a recombinant anti-TNF fusion protein is biologically active in the gut promoting regulatory T cells: results of a phase I clinical trial using a novel oral anti-TNFα—based therapy. J Immunol Methods 446:21–29. (PMID: 28392436)
Bortesi L, Fischer R (2015) The CRISPR/Cas9 system for plant genome editing and beyond. Biotechnol Adv 33:41–52. (PMID: 25536441)
Bosch D, Castilho A, Loos A, Schots A, Steinkellner H (2013) N-glycosylation of plant-produced recombinant proteins. Curr Pharm Des 19:5503–5512. (PMID: 23394562)
Cacciatore JJ, Chasin LA, Leonard EF (2010) Gene amplification and vector engineering to achieve rapid and high-level therapeutic protein production using the DHFR-based CHO cell selection system. Biotechnol Adv 28:673–681. (PMID: 20416368)
Cai CQ, Doyon Y, Ainley WM, Miller JC, Dekelver RC, Moehle EA, Rock JM, Lee YL, Garrison R, Schulenberg L, Blue R, Worden A, Baker L, Faraji F, Zhang L, Holmes MC, Rebar EJ, Collingwood TN, Rubin-Wilson B, Gregory PD, Urnov FD, Petolino JF (2009) Targeted transgene integration in plant cells using designed zinc finger nucleases. Plant Mol Biol 69:699–709. (PMID: 19112554)
Castilho A, Steinkellner H (2012) Glyco-engineering in plants to produce human-like N-glycan structures. Biotechnol J 7:1088–1098. (PMID: 22890723)
Castilho A, Gattinger P, Grass J, Jez J, Pabst M, Altmann F, Gorfer M, Strasser R, Steinkellner H (2011) N-glycosylation engineering of plants for the biosynthesis of glycoproteins with bisected and branched complex N-glycans. Glycobiology 21:813–823. (PMID: 213172433091529)
Cox KM, Sterling JD, Regan JT, Gasdaska JR, Frantz KK, Peele CG, Black A, Passmore D, Moldovan-Loomis C, Srinivasan M, Cuison S, Cardarelli PM, Dickey LF (2006) Glycan optimization of a human monoclonal antibody in the aquatic plant Lemna minor. Nat Biotechnol 24:1591–1597. (PMID: 17128273)
Delannoy M, Alves G, Vertommen D, Ma J, Boutry M, Navarre C (2008) Identification of peptidases in Nicotiana tabacum leaf intercellular fluid. Proteomics 8:2285–2298. (PMID: 18446799)
Duwadi K, Chen L, Menassa R, Dhaubhadel S (2015) Identification, characterization and down-regulation of cysteine protease genes in tobacco for use in recombinant protein production. PLoS ONE 10:e0130556. (PMID: 261480644493103)
El-Mounadi K, Morales-Floriano ML, Garcia-Ruiz H (2020) Principles, applications, and biosafety of plant genome editing using CRISPR-Cas9. Front Plant Sci 11:56. (PMID: 321173927031443)
Fischer R, Buyel JF (2020) Molecular farming—the slope of enlightenment. Biotechnol Adv 40:107519. (PMID: 31954848)
Fischer R, Schillberg S, Buyel JF, Twyman RM (2013) Commercial aspects of pharmaceutical protein production in plants. Curr Pharm Des 19:5471–5477. (PMID: 23394566)
Fu LH, Miao YS, Lo SW, Seto TC, Sun SSM, Xu ZF, Clemens S, Clarke LA, Kermode AR, Jang L (2009) Production and characterization of soluble human lysosomal enzyme α-iduronidase with high activity from culture media of transgenic tobacco BY-2 cells. Plant Sci 177:668–675.
Gecchele E, Merlin M, Brozzetti A, Falorni A, Pezzotti M, Avesani L (2015) A comparative analysis of recombinant protein expression in different biofactories: bacteria, insect cells and plant systems. J Vis Exp 97:52459.
Gengenbach BB, Keil LL, Opdensteinen P, Muschen CR, Melmer G, Lentzen H, Buhrmann J, Buyel JF (2019) Comparison of microbial and transient expression (tobacco plants and plant-cell packs) for the production and purification of the anticancer mistletoe lectin viscumin. Biotechnol Bioeng 116:2236–2249. (PMID: 311405806772165)
Gomord V, Fitchette AC, Menu-Bouaouiche L, Saint-Jore-Dupas C, Plasson C, Michaud D, Faye L (2010) Plant-specific glycosylation patterns in the context of therapeutic protein production. Plant Biotechnol J 8:564–587. (PMID: 20233335)
Hahn F, Nekrasov V (2019) CRISPR/Cas precision: do we need to worry about off-targeting in plants? Plant Cell Rep 38:437–441. (PMID: 30426198)
Hakkinen ST, Reuter L, Nuorti N, Joensuu JJ, Rischer H, Ritala A (2018) Tobacco BY-2 media component optimization for a cost-efficient recombinant protein production. Front Plant Sci 9:45. (PMID: 294346175791008)
Hanania U, Ariel T, Tekoah Y, Fux L, Sheva M, Gubbay Y, Weiss M, Oz D, Azulay Y, Turbovski A, Forster Y, Shaaltiel Y (2017) Establishment of a tobacco BY2 cell line devoid of plant-specific xylose and fucose as a platform for the production of biotherapeutic proteins. Plant Biotechnol J 15:1120–1129. (PMID: 281603635552476)
Havenith H, Raven N, Di Fiore S, Fischer R, Schillberg S (2014) Image-based analysis of cell-specific productivity for plant cell suspension cultures. Plant Cell Tiss Org Cult 117:393–399.
Hehle VK, Lombardi R, van Dolleweerd CJ, Paul MJ, Di Micco P, Morea V, Benvenuto E, Donini M, Ma JK (2015) Site-specific proteolytic degradation of IgG monoclonal antibodies expressed in tobacco plants. Plant Biotechnol J 13:235–245. (PMID: 25283551)
Hellwig S, Drossard J, Twyman RM, Fischer R (2004) Plant cell cultures for the production of recombinant proteins. Nat Biotechnol 22:1415–1422. (PMID: 15529167)
Holland T, Sack M, Rademacher T, Schmale K, Altmann F, Stadlmann J, Fischer R, Hellwig S (2010) Optimal nitrogen supply as a key to increased and sustained production of a monoclonal full-size antibody in BY-2 suspension culture. Biotechnol Bioeng 107:278–289. (PMID: 20506104)
Huang TK, McDonald KA (2009) Bioreactor engineering for recombinant protein production in plant cell suspension cultures. Biochem Eng J 45:168–184.
Huang TK, Plesha MA, Falk BW, Dandekar AM, McDonald KA (2009) Bioreactor strategies for improving production yield and functionality of a recombinant human protein in transgenic tobacco cell cultures. Biotechnol Bioeng 102:508–520. (PMID: 18767186)
Huang LF, Tan CC, Yeh JF, Liu HY, Liu YK, Ho SL, Lu CA (2015) Efficient secretion of recombinant proteins from rice suspension-cultured cells modulated by the choice of signal peptide. PLoS ONE 10:e0140812. (PMID: 264737224608814)
Ilan Y, Gingis-Velitski S, Ben Ya’aco A, Shabbat Y, Zolotarov L, Almon E, Shaaltiel Y (2017) A plant cell-expressed recombinant anti-TNF fusion protein is biologically active in the gut and alleviates immune-mediated hepatitis and colitis. Immunobiology 222:544–551. (PMID: 27832933)
Imamura T, Isozumi N, Higashimura Y, Ohki S, Mori M (2021) Production of ORF8 protein from SARS-CoV-2 using an inducible virus-mediated expression system in suspension-cultured tobacco BY-2 cells. Plant Cell Rep. https://doi.org/10.1007/s00299-020-02654-5. (PMID: 10.1007/s00299-020-02654-5333998797783501)
Jansing J, Sack M, Augustine SM, Fischer R, Bortesi L (2019) CRISPR/Cas9-mediated knockout of six glycosyltransferase genes in Nicotiana benthamiana for the production of recombinant proteins lacking β-1, 2-xylose and core α-1, 3-fucose. Plant Biotechnol J 17:350–361. (PMID: 29969180)
Jung JW, Shin JH, Lee WK, Begum H, Min CH, Jang MH, Oh HB, Yang MS, Kim SR (2021) Inactivation of the beta (1, 2)-xylosyltransferase and the alpha (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy. Plant Cell Rep. https://doi.org/10.1007/s00299-021-02667-8. (PMID: 10.1007/s00299-021-02667-833547931)
Jutras PV, Grosse-Holz F, Kaschani F, Kaiser M, Michaud D, van der Hoorn RAL (2019) Activity-based proteomics reveals nine target proteases for the recombinant protein-stabilizing inhibitor SlCYS8 in Nicotiana benthamiana. Plant Biotechnol J 17:1670–1678. (PMID: 307427306662110)
Kaldis A, Ahmad A, Reid A, McGarvey B, Brandle J, Ma S, Jevnikar A, Kohalmi SE, Menassa R (2013) High-level production of human interleukin-10 fusions in tobacco cell suspension cultures. Plant Biotechnol J 11:535–545. (PMID: 232976983712471)
Kallolimath S, Castilho A, Strasser R, Grunwald-Gruber C, Altmann F, Strubl S, Galuska CE, Zlatina K, Galuska SP, Werner S, Thiesler H, Werneburg S, Hildebrandt H, Gerardy-Schahn R, Steinkellner H (2016) Engineering of complex protein sialylation in plants. Proc Natl Acad Sci U S A 113:9498–9503. (PMID: 274440135003249)
Kato K, Matsumoto T, Koiwai A, Mizusaki S, Nishida K, Noguchi M, Tamaki E (1972) Liquid suspension culture of tobaco cells. Fermentation technology today: Proceedings of the IVth International Fermentation Symposium, Kyoto, Japan, pp 689–695.
Khoudi H, Laberge S, Ferullo JM, Bazin R, Darveau A, Castonguay Y, Allard G, Lemieux R, Vezina LP (1999) Production of a diagnostic monoclonal antibody in perennial alfalfa plants. Biotechnol Bioeng 64:135–143. (PMID: 10397849)
Kim NS, Jang SH, Yu HY, Chung ND, Kwon TH, Yang MS, Kim TG (2013) Amylase and cysteine proteinase gene knockdown in rice cells using RNA interference for enhancing production of recombinant proteins. Plant Cell Tiss Org Cult 114:97–107.
Kirchhoff J, Raven N, Boes A, Roberts JL, Russell S, Treffenfeldt W, Fischer R, Schinkel H, Schiermeyer A, Schillberg S (2012) Monoclonal tobacco cell lines with enhanced recombinant protein yields can be generated from heterogeneous cell suspension cultures by flow sorting. Plant Biotechnol J 10:936–944. (PMID: 22758383)
Klimek-Chodacka M, Oleszkiewicz T, Lowder LG, Qi Y, Baranski R (2018) Efficient CRISPR/Cas9-based genome editing in carrot cells. Plant Cell Rep 37:575–586. (PMID: 293321685852178)
Kwon TH, Seo JE, Kim J, Lee JH, Jang YS, Yang MS (2003) Expression and secretion of the heterodimeric protein interleukin-12 in plant cell suspension culture. Biotechnol Bioeng 81:870–875. (PMID: 12557321)
Lallemand J, Bouche F, Desiron C, Stautemas J, de Lemos EF, Perilleux C, Tocquin P (2015) Extracellular peptidase hunting for improvement of protein production in plant cells and roots. Front Plant Sci 6:37. (PMID: 257052124319384)
Li JF, Norville JE, Aach J, McCormack M, Zhang D, Bush J, Church GM, Sheen J (2013) Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nat Biotechnol 31:688–691. (PMID: 239293394078740)
Li H, Guan R, Guo H, Miao X (2015) New insights into an RNAi approach for plant defence against piercing-sucking and stem-borer insect pests. Plant Cell Environ 38:2277–2285. (PMID: 25828885)
Li J, Stoddard TJ, Demorest ZL, Lavoie PO, Luo S, Clasen BM, Cedrone F, Ray EE, Coffman AP, Daulhac A, Yabandith A, Retterath AJ, Mathis L, Voytas DF, D’Aoust MA, Zhang F (2016) Multiplexed, targeted gene editing in Nicotiana benthamiana for glyco-engineering and monoclonal antibody production. Plant Biotechnol J 14:533–542. (PMID: 26011187)
Liang G, Zhang H, Lou D, Yu D (2016) Selection of highly efficient sgRNAs for CRISPR/Cas9-based plant genome editing. Sci Rep 6:21451. (PMID: 268916164759811)
Lico C, Santi L, Baschieri S, Noris E, Marusic C, Donini M, Pedrazzini E, Maga G, Franconi R, Di Bonito P, Avesani L (2020) Plant molecular farming as a strategy against COVID-19—the Italian perspective. Front Plant Sci 11:609910. (PMID: 333811407768017)
Liu W, Rudis MR, Cheplick MH, Millwood RJ, Yang JP, Ondzighi-Assoume CA, Montgomery GA, Burris KP, Mazarei M, Chesnut JD, Stewart CN Jr (2020) Lipofection-mediated genome editing using DNA-free delivery of the Cas9/gRNA ribonucleoprotein into plant cells. Plant Cell Rep 39:245–257. (PMID: 31728703)
Macharoen K, Li Q, Marquez-Escobar VA, Corbin JM, Lebrilla CB, Nandi S, McDonald KA (2020) Effects of kifunensine on production and N-glycosylation modification of butyrylcholinesterase in a transgenic rice cell culture bioreactor. Int J Mol Sci 21:6896. (PMID: 7555773)
Magy B, Tollet J, Laterre R, Boutry M, Navarre C (2014) Accumulation of secreted antibodies in plant cell cultures varies according to the isotype, host species and culture conditions. Plant Biotechnol J 12:457–467. (PMID: 24373507)
Mandal MK, Fischer R, Schillberg S, Schiermeyer A (2014) Inhibition of protease activity by antisense RNA improves recombinant protein production in Nicotiana tabacum cv. Bright yellow 2 (BY-2) suspension cells. Biotechnol J 9:1065–1073. (PMID: 24828029)
Mandal MK, Ahvari H, Schillberg S, Schiermeyer A (2016) Tackling unwanted proteolysis in plant production hosts used for molecular farming. Front Plant Sci 7:267. (PMID: 270142934782010)
McBride KE, Schaaf DJ, Daley M, Stalker DM (1994) Controlled expression of plastid transgenes in plants based on a nuclear DNA-encoded and plastid-targeted T7 RNA polymerase. Proc Natl Acad Sci USA 91:7301–7305. (PMID: 804178444387)
McDonald KA, Hong LM, Trombly DM, Xie Q, Jackman AP (2005) Production of human α-1-antitrypsin from transgenic rice cell culture in a membrane bioreactor. Biotechnol Prog 21:728–734. (PMID: 15932249)
Mercx S, Tollet J, Magy B, Navarre C, Boutry M (2016) Gene inactivation by CRISPR-Cas9 in Nicotiana tabacum BY-2 suspension cells. Front Plant Sci 7:40. (PMID: 268700614734102)
Modrzejewski D, Hartung F, Lehnert H, Sprink T, Kohl C, Keilwagen J, Wilhelm R (2020) Which factors affect the occurrence of off-target effects caused by the use of CRISPR/Cas: a systematic review in plants. Front Plant Sci 11:574959. (PMID: 333296347719684)
Nguyen HT, Leelavathi S, Reddy VS (2004) Bacteriophage T7 RNA polymerase-directed, inducible and tissue-specific over-expression of foreign genes in transgenic plants. Plant Biotechnol J 2:301–310. (PMID: 17134391)
Oberemok VV, Laikova KV, Repetskaya AI, Kenyo IM, Gorlov MV, Kasich IN, Krasnodubets AM, Gal’chinsky NV, Fomochkina II, Zaitsev AS, Bekirova VV, Seidosmanova EE, Dydik KI, Meshcheryakova AO, Nazarov SA, Smagliy NN, Chelengerova EL, Kulanova AA, Deri K, Subbotkin MV, Useinov RZ, Shumskykh MN, Kubyshkin AV (2018) A half-century history of applications of antisense oligonucleotides in medicine, agriculture and forestry: we should continue the journey. Molecules 23(6):1302. (PMID: 6099785)
Ogawa Y, Suzuki H, Sakurai N, Aoki K, Saito K, Shibata D (2008) Cryopreservation and metabolic profiling analysis of Arabidopsis T87 suspension-cultured cells. Cryo Lett 29:427–436.
Paccalet T, Bardor M, Rihouey C, Delmas F, Chevalier C, D’Aoust MA, Faye L, Vezina L, Gomord V, Lerouge P (2007) Engineering of a sialic acid synthesis pathway in transgenic plants by expression of bacterial Neu5Ac-synthesizing enzymes. Plant Biotechnol J 5:16–25. (PMID: 17207253)
Park YJ, Han JE, Lee H, Jung YJ, Murthy HN, Park SY (2020) Large-scale production of recombinant miraculin protein in transgenic carrot callus suspension cultures using air-lift bioreactors. AMB Express 10:140. (PMID: 327897047426344)
Permyakova NV, Sidorchuk YV, Marenkova TV, Khozeeva SA, Kuznetsov VV, Zagorskaya AA, Rozov SM, Deineko EV (2019) CRISPR/Cas9-mediated GFP gene inactivation in Arabidopsis suspension cells. Mol Biol Rep 46:5735–5743. (PMID: 31392536)
Pham PV (2018) Medical biotechnology: techniques and applications. In: Azevedo V, Barh D (eds) Omics technologies and bio-engineering: towards improving quqlity of life. Elsevier Science, pp 449–469.
Pillay P, Schluter U, van Wyk S, Kunert KJ, Vorster BJ (2014) Proteolysis of recombinant proteins in bioengineered plant cells. Bio Eng 5:15–20.
Pires AS, Rosa S, Castanheira S, Fevereiro P, Abranches R (2012) Expression of a recombinant human erythropoietin in suspension cell cultures of Arabidopsis, tobacco and medicago. Plant Cell Tiss Org Cult 110:171–181.
Poborilova Z, Plchova H, Cerovska N, Gunter CJ, Hitzeroth II, Rybicki EP, Moravec T (2020) Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors. Plant Cell Rep 39:1115–1127. (PMID: 323331517223956)
Rademacher T, Sack M, Blessing D, Fischer R, Holland T, Buyel J (2019) Plant cell packs: a scalable platform for recombinant protein production and metabolic engineering. Plant Biotechnol J 17:1560–1566. (PMID: 306720786662111)
Raven N, Rasche S, Kuehn C, Anderlei T, Klockner W, Schuster F, Henquet M, Bosch D, Buchs J, Fischer R, Schillberg S (2015) Scaled-up manufacturing of recombinant antibodies produced by plant cells in a 200-L orbitally-shaken disposable bioreactor. Biotechnol Bioeng 112:308–321. (PMID: 25117428)
Rawlings ND, Waller M, Barrett AJ, Bateman A (2014) MEROPS: the database of proteolytic enzymes, their substrates and inhibitors. Nucleic Acids Res 42:D503-509. (PMID: 24157837)
Rawlings ND, Barrett AJ, Finn R (2016) Twenty years of the MEROPS database of proteolytic enzymes, their substrates and inhibitors. Nucleic Acids Res 44:D343-350. (PMID: 26527717)
Ren C, Liu X, Zhang Z, Wang Y, Duan W, Li S, Liang Z (2016) CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.). Sci Rep 6(1):32289. (PMID: 275768935006071)
Reuter LJ, Bailey MJ, Joensuu JJ, Ritala A (2014) Scale-up of hydrophobin-assisted recombinant protein production in tobacco BY-2 suspension cells. Plant Biotechnol J 12:402–410. (PMID: 24341724)
Reuter LJ, Conley AJ, Joensuu JJ (2016) Continuous flow separation of hydrophobin fusion proteins from plant cell culture extract. Methods Mol Biol 1385:189–197. (PMID: 26614291)
Sadoch J, Pyc M, Urbanowicz A, Iglewski A, Pilarski R (2021) High-throughput evolutionary optimization of the induction medium towards recombinant protein production in BY-2 tobacco. Biotechnol Bioeng 118:676–689. (PMID: 33038011)
Santos RB, Abranches R, Fischer R, Sack M, Holland T (2016) Putting the spotlight back on plant suspension cultures. Front Plant Sci 7:297. (PMID: 270143204786539)
Schiermeyer A, Schinkel H, Apel S, Fischer R, Schillberg S (2005) Production of Desmodus rotundus salivary plasminogen activator α1 (DSPAα1) in tobacco is hampered by proteolysis. Biotechnol Bioeng 89:848–858. (PMID: 15685597)
Schillberg S, Finnern R (2021) Plant molecular farming for the production of valuable proteins—critical evaluation of achievements and future challenges. J Plant Physiol 258–259:153359. (PMID: 33460995)
Schillberg S, Raven N, Spiegel H, Rasche S, Buntru M (2019) Critical analysis of the commercial potential of plants for the production of recombinant proteins. Front Plant Sci 10:720. (PMID: 312448686579924)
Schumacher HM, Westphal M, Heine-Dobbernack E (2015) Cryopreservation of plant cell lines. Methods Mol Biol 1257:423–429. (PMID: 25428021)
Shaaltiel Y, Tekoah Y (2016) Plant specific N-glycan do not have proven adverse effects in humans. Nat Biotechnol 34:17–18.
Sheva M, Hanania U, Ariel T, Turbovski A, Rathod VKR, Oz D, Tekoah Y, Shaaltiel Y (2020) Sequential genome editing and induced excision of the transgene in N. tabacum BY2 cells. Front Plant Sci. https://doi.org/10.3389/fpls.2020.60714. (PMID: 10.3389/fpls.2020.60714333244407723889)
Shih SMH, Doran PM (2009) Foreign protein production using plant cell and organ cultures: advantages and limitations. Biotechnol Adv 27:1036–1042. (PMID: 19463933)
Shin YJ, Chong YJ, Yang MS, Kwon TH (2011) Production of recombinant human granulocyte macrophage-colony stimulating factor in rice cell suspension culture with a human-like N-glycan structure. Plant Biotechnol J 9:1109–1119. (PMID: 21801300)
Silva AT, Nguyen A, Ye C, Verchot J, Moon JH (2010) Conjugated polymer nanoparticles for effective siRNA delivery to tobacco BY-2 protoplasts. BMC Plant Biol 10:291. (PMID: 211928273023792)
Small I (2007) RNAi for revealing and engineering plant gene functions. Curr Opin Biotechnol 18:148–153. (PMID: 17287115)
Sojikul P, Buehner N, Mason HS (2003) A plant signal peptide–hepatitis B surface antigen fusion protein with enhanced stability and immunogenicity expressed in plant cells. Proc Natl Acad Sci USA 100:2209–2214. (PMID: 12601177151319)
Strasser R (2016) Plant protein glycosylation. Glycobiology 26:926–939. (PMID: 269112865045529)
Sukenik SC, Karuppanan K, Li Q, Lebrilla CB, Nandi S, McDonald KA (2018) Transient recombinant protein production in glycoengineered Nicotiana benthamiana cell suspension culture. Int J Mol Sci 19:120.
Sun Q-Y, Ding L-W, Lomonossoff GP, Sun Y-B, Luo M, Li C-Q, Jiang L, Xu Z-F (2011) Improved expression and purification of recombinant human serum albumin from transgenic tobacco suspension culture. J Biotechnol 155:164–172. (PMID: 21762733)
Tekoah Y, Shulman A, Kizhner T, Ruderfer I, Fux L, Nataf Y, Bartfeld D, Ariel T, Gingis-Velitski S, Hanania U, Shaaltiel Y (2015) Large-scale production of pharmaceutical proteins in plant cell culture-the protalix experience. Plant Biotechnol J 13:1199–1208. (PMID: 26102075)
Vasilev N, Grömping U, Lipperts A, Raven N, Fischer R, Schillberg S (2013) Optimization of BY-2 cell suspension culture medium for the production of a human antibody using a combination of fractional factorial designs and the response surface method. Plant Biotechnol J 11:867–874. (PMID: 23721307)
Vaucheret H (2015) Gene silencing: mode of miRNA biogenesis matters. Nat Plants 1:15019. (PMID: 27246886)
Wada N, Ueta R, Osakabe Y, Osakabe K (2020) Precision genome editing in plants: state-of-the-art in CRISPR/Cas9-based genome engineering. BMC Plant Biol 20:234. (PMID: 324508027249668)
Wang X, Karki U, Abeygunaratne H, UnnoldCofre C, Xu J (2021) Plant cell-secreted stem cell factor stimulates expansion and differentiation of hematopoietic stem cells. Process Biochem 100:39–48. (PMID: 33071562)
Wilson SA, Roberts SC (2012) Recent advances towards development and commercialization of plant cell culture processes for the synthesis of biomolecules. Plant Biotechnol J 10:249–268. (PMID: 22059985)
Xu J, Zhang N (2014) On the way to commercializing plant cell culture platform for biopharmaceuticals: present status and prospect. Pharma Bioprocess 2:499–518.
Xu J, Tan L, Goodrum KJ, Kieliszewski MJ (2007) High-yields and extended serum half-life of human interferon α2b expressed in tobacco cells as arabinogalactan-protein fusions. Biotechnol Bioeng 97:997–1008. (PMID: 17328066)
Xu J, Okada S, Tan L, Goodrum KJ, Kopchick JJ, Kieliszewski MJ (2010) Human growth hormone expressed in tobacco cells as an arabinogalactan-protein fusion glycoprotein has a prolonged serum life. Transgenic Res 19:849–867. (PMID: 20135224)
Xu J, Ge X, Dolan MC (2011) Towards high-yield production of pharmaceutical proteins with plant cell suspension cultures. Biotechnol Adv 29:278–299. (PMID: 21236330)
Xu J, Dolan MC, Medrano G, Cramer CL, Weathers PJ (2012) Green factory: plants as bioproduction platforms for recombinant proteins. Biotechnol Adv 30:1171–1184. (PMID: 21924345)
Xu J, Towler M, Weathers PJ (2016) Platforms for plant-based protein production. In: Pavlov A, Bley T (eds) Bioprocessing of plant in vitro systems reference series in phytochemistry. Springer International Publishing AG, pp 1–40.
Yano A, Maeda F, Takekoshi M (2004) Transgenic tobacco cells producing the human monoclonal antibody to hepatitis B virus surface antigen. J Med Virol 73:208–215. (PMID: 15122794)
Yin BJ, Gao T, Zheng NY, Li Y, Tang SY, Liang LM, Xie Q (2011) Generation of glyco-engineered BY2 cell lines with decreased expression of plant-specific glycoepitopes. Protein Cell 2:41–47. (PMID: 213370084875288)
Younis A, Siddique MI, Kim CK, Lim KB (2014) RNA interference (RNAi) induced gene silencing: a promising approach of hi-tech plant breeding. Int J Biol Sci 10:1150–1158. (PMID: 253326894202031)
Yu XD, Liu ZC, Huang SL, Chen ZQ, Sun YW, Duan PF, Ma YZ, Xia LQ (2016) RNAi-mediated plant protection against aphids. Pest Manag Sci 72:1090–1098. (PMID: 26888776)
Zhang N, Dolan M, Wu D, Phillips GC, Xu J (2016a) Dramatic secretion of recombinant protein expressed in tobacco cells with a designer glycopeptide tag is highly impacted by medium composition. Plant Cell Rep 35:2513–2522. (PMID: 27632186)
Zhang N, Gonzalez M, Savary B, Xu J (2016b) High-yield secretion of recombinant proteins expressed in tobacco cell culture with a designer glycopeptide tag: process development. Biotechnol J 11:497–506. (PMID: 26627201)
Zhang N, Wright T, Caraway P, Xu J (2019) Enhanced secretion of human α1-antitrypsin expressed with a novel glycosylation module in tobacco BY-2 cell culture. Bio Eng 10:87–97. - Grant Information: P20 GM103429 United States GM NIGMS NIH HHS; P20GM103429 NIH-INBRE; 1605564 National Science Foundation
- Contributed Indexing: Keywords: Cellular engineering; Genome editing; Glycoengineering; Plant cell culture; RNA interference; Recombinant proteins
- الرقم المعرف: 0 (Recombinant Proteins)
- الموضوع: Date Created: 20210410 Date Completed: 20210716 Latest Revision: 20210716
- الموضوع: 20221213
- الرقم المعرف: PMC8035600
- الرقم المعرف: 10.1007/s00299-021-02693-6
- الرقم المعرف: 33837823
- المصدر:
حقوق النشر© 2024، دائرة الثقافة والسياحة جميع الحقوق محفوظة Powered By EBSCO Stacks 3.3.0 [353] | Staff Login
![](https://library.dctabudhabi.ae/sites/default/files/dct_logo_white.png)
حقوق النشر © دائرة الثقافة والسياحة، جميع الحقوق محفوظة
No Comments.