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Costunolide attenuates oxygen‑glucose deprivation/reperfusion‑induced mitochondrial‑mediated apoptosis in PC12 cells.

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  • معلومة اضافية
    • المصدر:
      Publisher: D. A. Spandidos Country of Publication: Greece NLM ID: 101475259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1791-3004 (Electronic) Linking ISSN: 17912997 NLM ISO Abbreviation: Mol Med Rep Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Athens, Greece : D. A. Spandidos
    • الموضوع:
      Cell Hypoxia*; Apoptosis/*drug effects ; Glucose/*deficiency ; Mitochondria/*metabolism ; Neuroprotective Agents/*pharmacology ; Sesquiterpenes/*pharmacology; Animals ; Calcium/metabolism ; Caspases/metabolism ; L-Lactate Dehydrogenase/metabolism ; Membrane Potential, Mitochondrial ; PC12 Cells ; Rats
    • نبذة مختصرة :
      The present study investigated the effect of costunolide (CT), a compound extracted from A ucklandia lappa Decne, to attenuate oxygen‑glucose deprivation/reperfusion (OGD/R)‑induced mitochondrial‑mediated apoptosis in PC12 cells. The present study used molecular docking technology to detect the binding of CT with mitochondrial apoptotic protein targets. A model of oxygen‑glucose deprivation for 2 h and reperfusion for 24 h in PC12 cells was used to mimic cerebral ischemic injury. Cell viability and damage were measured using the Cell Counting kit‑8 and lactate dehydrogenase (LDH) cytotoxicity assay kits. Cellular apoptosis was analyzed using flow cytometry. A fluorescence microscope determined intracellular [Ca 2+ ] and mitochondrial membrane potential. Furthermore, immunofluorescence and Western blot analyses were used to detect the expression of apoptosis‑associated proteins. CT contains binding sites with Caspase‑3, Caspase‑9 and Caspase‑7. CT markedly enhanced cell viability, inhibited LDH leakage, increased intracellular [Ca 2+ ], stabilized the mitochondrial membrane potential, increased the expression of Bcl‑2 and inhibited the expression of Apaf‑1, Bax, cleaved‑caspase‑7, cleaved‑caspase‑9 and cleaved‑caspase‑3. CT may markedly protect PC12 cells from damage caused by OGD/R, and its mechanism is associated with blocking the calcium channel and inhibiting mitochondrial‑mediated apoptosis.
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    • Contributed Indexing:
      Keywords: costunolide; ischemic stroke; oxygen‑glucose deprivation/reperfusion; apoptosis; caspase; PC12 cells
    • الرقم المعرف:
      0 (Neuroprotective Agents)
      0 (Sesquiterpenes)
      4IK578SA7Z (costunolide)
      EC 1.1.1.27 (L-Lactate Dehydrogenase)
      EC 3.4.22.- (Caspases)
      IY9XDZ35W2 (Glucose)
      SY7Q814VUP (Calcium)
    • الموضوع:
      Date Created: 20210331 Date Completed: 20210510 Latest Revision: 20210916
    • الموضوع:
      20250114
    • الرقم المعرف:
      PMC8025489
    • الرقم المعرف:
      10.3892/mmr.2021.12050
    • الرقم المعرف:
      33786628