Human Interferon Inducible Transmembrane Protein 3 (IFITM3) Inhibits Influenza Virus A Replication and Inflammation by Interacting with ABHD16A.

Publisher: Hindawi Pub. Co Country of Publication: United States NLM ID: 101600173 Publication Model: eCollection Cited Medium: Internet ISSN: 2314-6141 (Electronic) NLM ISO Abbreviation: Biomed Res Int Subsets: MEDLINE

Original Publication: New York, NY : Hindawi Pub. Co.

Virus Replication*; Influenza A virus/*physiology ; Membrane Proteins/*metabolism ; Monoacylglycerol Lipases/*metabolism ; RNA-Binding Proteins/*metabolism; HEK293 Cells ; Humans ; Inflammation/genetics ; Inflammation/metabolism ; Inflammation/pathology ; Inflammation/virology ; Membrane Proteins/genetics ; Monoacylglycerol Lipases/genetics ; RNA-Binding Proteins/genetics

Studies have shown that human interferon inducible transmembrane protein (hIFITMs) family proteins have broad-spectrum antiviral capabilities. Preliminary studies in our laboratory have tentatively proved that hIFITMs have the effect of inhibiting influenza viruses. In order to further study its mechanism and role in the occurrence and development of influenza A, relevant studies have been carried out. Fluorescence quantitative polymerase chain reaction (PCR) detection technology was used to observe the effect of hIFITM3 on the replication of influenza A virus (IVA) and the interaction with hABHD16A. In HEK293 cells, overexpression of hIFITM3 protein significantly inhibited the replication of IVA at 24 h, 48 h, and 72 h; yeast two-hybrid experiment proved that hIFITM3 interacts with hABHD16A; laser confocal microscopy observations showed that hIFITM3 and hABHD16A colocalized in the cell membrane area; the expression level of inflammation-related factors in cells overexpressing hIFITM3 or hABHD16A was detected by fluorescence quantitative PCR, and the results showed that the mRNA levels of interleukin- (IL-) 1 β , IL-6, IL-10, tumor necrosis factor- (TNF-) α , and cyclooxygenase 2 (COX2) were significantly increased. But when hIFITM3/hABHD16A was coexpressed, the mRNA expression levels of these cytokines were significantly reduced except COX2. When influenza virus infected cells coexpressing hIFITM3/hABHD16A, the expression level of inflammatory factors decreased compared with the control group, indicating that hIFITM3 can play an important role in regulating inflammation balance. This study confirmed that hIFITM3 has an effect of inhibiting IVA replication. Furthermore, it was found that hIFITM3 interacts with hABHD16A, following which it can better inhibit the replication of influenza virus and the inflammatory response caused by the disease process.
Competing Interests: The authors declare that there is no conflict of interest regarding the publication of this paper.
(Copyright © 2021 Liang Chen et al.)

Retraction in: Biomed Res Int. 2022 Apr 6;2022:9790782. (PMID: 35434131)

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0 (IFITM3 protein, human)
0 (Membrane Proteins)
0 (RNA-Binding Proteins)
EC 3.1.1.23 (ABHD16A protein, human)
EC 3.1.1.23 (Monoacylglycerol Lipases)

Date Created: 20210325 Date Completed: 20210526 Latest Revision: 20220418

20240829

PMC7946484

10.1155/2021/6652147

33763481