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Genome-wide identification and expression analysis of the Brassica oleracea L. chitin-binding genes and response to pathogens infections.

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  • معلومة اضافية
    • المصدر:
      Publisher: Springer-Verlag [etc.] Country of Publication: Germany NLM ID: 1250576 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-2048 (Electronic) Linking ISSN: 00320935 NLM ISO Abbreviation: Planta Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Berlin, New York, Springer-Verlag [etc.]
    • الموضوع:
      Brassica*/genetics ; Brassica*/microbiology ; Genome, Plant* ; Plant Immunity*; Chitin/*metabolism ; Plant Proteins/*genetics; Chitinases/genetics ; Gene Expression Regulation, Plant ; Phylogeny
    • نبذة مختصرة :
      Main Conclusion: Chitinase family genes were involved in the response of Brassica oleracea to Fusarium wilt, powdery mildew, black spot and downy mildew. Abstract Chitinase, a category of pathogenesis-related proteins, is believed to play an important role in defending against external stress in plants. However, a comprehensive analysis of the chitin-binding gene family has not been reported to date in cabbage (Brassica oleracea L.), especially regarding the roles that chitinases play in response to various diseases. In this study, a total of 20 chitinase genes were identified using a genome-wide search method. Phylogenetic analysis was employed to classify these genes into two groups. The genes were distributed unevenly across six chromosomes in cabbage, and all of them contained few introns (≤ 2). The results of collinear analysis showed that the cabbage genome contained 1-5 copies of each chitinase gene (excluding Bol035470) identified in Arabidopsis. The heatmap of the chitinase gene family showed that these genes were expressed in various tissues and organs. Two genes (Bol023322 and Bol041024) were relatively highly expressed in all of the investigated tissues under normal conditions, exhibiting the expression characteristics of housekeeping genes. In addition, under four different stresses, namely, Fusarium wilt, powdery mildew, black spot and downy mildew, we detected 9, 5, 8 and 8 genes with different expression levels in different treatments, respectively. Our results may help to elucidate the roles played by chitinases in the responses of host plants to various diseases.
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    • Grant Information:
      Grant No. 31801876 the Youth Science Fund Project; Grant No. 2017YFD0101804 the National Key Research and Development Program of China
    • Contributed Indexing:
      Keywords: Cabbage; Chitinase family genes; Diseases; Genome-wide
    • الرقم المعرف:
      0 (Plant Proteins)
      1398-61-4 (Chitin)
      EC 3.2.1.14 (Chitinases)
    • الموضوع:
      Date Created: 20210320 Date Completed: 20210402 Latest Revision: 20220421
    • الموضوع:
      20231215
    • الرقم المعرف:
      PMC7979657
    • الرقم المعرف:
      10.1007/s00425-021-03596-2
    • الرقم المعرف:
      33742226