Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Induction of macrophage pyroptosis-related factors by pathogenic E. coli high pathogenicity island (HPI) in Yunnan Saba pigs.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : BioMed Central, 2005-
    • الموضوع:
      Genomic Islands* ; Pyroptosis*; Escherichia coli/*pathogenicity ; Macrophages/*microbiology; Animals ; Apoptosis Regulatory Proteins/metabolism ; Caspase 1/metabolism ; Cell Line ; Cell Membrane/pathology ; China ; Escherichia coli/genetics ; Gene Expression Regulation ; Inflammation ; Lipopolysaccharides/pharmacology ; Macrophages/physiology ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism ; Signal Transduction ; Swine
    • نبذة مختصرة :
      Background: Pyroptosis plays a pivotal role in the pathogenesis of many inflammatory diseases. The molecular mechanism by which pyroptosis is induced in macrophages following infection with pathogenic E. coli high pathogenicity island (HPI) will be evaluated in our study.
      Results: After infection with the HPI + /HPI - strains and LPS, decreased macrophage cell membrane permeability and integrity were demonstrated with propidium iodide (PI) staining and the lactate dehydrogenase (LDH) assay. HPI + /HPI - -infection was accompanied by upregulated expression levels of NLRP3, ASC, caspase-1, IL-1β, IL-18 and GSDMD, with significantly higher levels detected in the HPI + group compared to those in the HPI - group (P < 0.01 or P < 0.05). HPI + strain is more pathogenic than HPI - strain.
      Conclusion: Our findings indicate that pathogenic E. coli HPI infection of Saba pigs causes pyroptosis of macrophages characterized by upregulated expression of pyroptosis key factors in the NLRP3/ASC/caspase-1 signaling pathway, direct cell membrane pore formation, and secretion of the inflammatory factor IL-1β and IL-18 downstream of NLRP3 and caspase-1 activation to enhance the inflammatory response.
    • References:
      Cell Microbiol. 2007 Nov;9(11):2562-70. (PMID: 17714514)
      Clin Microbiol Rev. 2013 Oct;26(4):822-80. (PMID: 24092857)
      Int J Ophthalmol. 2015 Feb 18;8(1):46-51. (PMID: 25709906)
      Nature. 2016 Jul 06;535(7610):153-8. (PMID: 27383986)
      Trends Biochem Sci. 2017 Apr;42(4):245-254. (PMID: 27932073)
      J Clin Microbiol. 2012 May;50(5):1673-8. (PMID: 22378905)
      PLoS One. 2017 Jul 3;12(7):e0180599. (PMID: 28671990)
      Immunopharmacol Immunotoxicol. 2019 Oct;41(5):538-548. (PMID: 31549524)
      Nature. 1992 Jul 9;358(6382):167-9. (PMID: 1614548)
      J Cell Death. 2018 Jan 03;11:1179066017750902. (PMID: 29434478)
      PLoS One. 2009 Dec 29;4(12):e8240. (PMID: 20041108)
      Nat Immunol. 2010 Dec;11(12):1136-42. (PMID: 21057511)
      Res Vet Sci. 2018 Oct;120:41-46. (PMID: 30199780)
      Nature. 2012 Jan 18;481(7381):278-86. (PMID: 22258606)
      Hepatology. 2014 Mar;59(3):898-910. (PMID: 23813842)
      Zhongguo Zhong Xi Yi Jie He Za Zhi. 2017 Apr;37(4):470-475. (PMID: 30650508)
      Mol Microbiol. 1997 Mar;23(6):1089-97. (PMID: 9106201)
      FEMS Immunol Med Microbiol. 2004 Jan 15;40(1):51-5. (PMID: 14734186)
      Nature. 2014 Oct 9;514(7521):187-92. (PMID: 25119034)
      PLoS One. 2014 Jan 16;9(1):e85765. (PMID: 24454930)
      Pediatr Rev. 2015 Apr;36(4):167-70; quiz 171. (PMID: 25834220)
      Nat Rev Immunol. 2016 Jan;16(1):7-21. (PMID: 26655628)
      Cell Res. 2015 Dec;25(12):1285-98. (PMID: 26611636)
      Nature. 2016 Jul 7;535(7610):111-6. (PMID: 27281216)
      Nature. 2008 Mar 6;452(7183):103-7. (PMID: 18288107)
      J Cell Physiol. 2019 Jun;234(6):7885-7892. (PMID: 30537070)
      J Immunol. 2012 Apr 15;188(8):3594-602. (PMID: 22403439)
      J Biol Chem. 2009 Jul 31;284(31):20574-81. (PMID: 19509280)
      Cell Host Microbe. 2010 Jul 22;8(1):44-54. (PMID: 20638641)
      Nephrology (Carlton). 2016 Sep;21(9):736-44. (PMID: 27011059)
      Cell Microbiol. 2008 Jan;10(1):41-52. (PMID: 17662073)
      Pathog Dis. 2015 Jun;73(4):. (PMID: 25834143)
    • Grant Information:
      31660704 and 31960692 National Natural Science Foundation of China; 2019Y0096 Yunnan Provincial Department of Education
    • Contributed Indexing:
      Keywords: Caspase-1; HPI; Macrophage; Pathogenic E. coli; Pyroptosis
    • الرقم المعرف:
      0 (Apoptosis Regulatory Proteins)
      0 (Lipopolysaccharides)
      0 (NLR Family, Pyrin Domain-Containing 3 Protein)
      EC 3.4.22.36 (Caspase 1)
    • الموضوع:
      Date Created: 20210308 Date Completed: 20210813 Latest Revision: 20210813
    • الموضوع:
      20221213
    • الرقم المعرف:
      PMC7938518
    • الرقم المعرف:
      10.1186/s12917-021-02824-x
    • الرقم المعرف:
      33678162