نبذة مختصرة : Two bacterial strains, designated REN4 T and REN4-1, were isolated from daqu sample collected from baijiu factory located in Shanxi, China. The two strains shared highly similar 16S rRNA gene sequences (99.67% identities) and formed a monophyletic clade within the Brevibacterium 16S rRNA gene tree, showing 97.56-97.85% 16S rRNA gene sequence identities with type strains Brevibacterium permense VKM Ac-2280 T , Brevibacterium sediminis FXJ8.269 T , Brevibacterium oceani BBH7 T and Brevibacterium epidermidis NCIMB 702286 T . They contained MK-8(H 2 ) as the most predominant menaquinone, antesio-C 15:0 , antesio-C 17:0 , Iso-C 16:0 and Iso-C 17:0 as the major cellular fatty acids, DPG (diphosphatidylglycerol), PG (phosphatidylglycerol), PGL (phosphatidylglycerollipids), and PL (phospholipids) as the main polar lipids. The genomic DNA G + C content of strains REN4 and REN4-1 were 64.35, 65.82 mol%. Moreover, the low DNA-DNA relatedness values, physiological and biochemical characteristics, and taxonomic analysis allowed the differentiation of strains REN4 T and REN4-1 from the other recognized species of the genus Brevibacterium. Therefore, strain REN4 T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium renqingii sp. nov. is proposed, with the type strain REN4 T (= JCM 33953 T = KCTC 49366 T ).
References: Auch AF, Klenk H, Goeker M (2010) Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs. STANDARDS IN GENOMIC SCIENCES 2:142–148. (PMID: 10.4056/sigs.541628)
Bhadra B, Raghukumar C, Pindi PK, Shivaji S (2008) Brevibacterium oceani sp. nov., isolated from deep-sea sediment of the Chagos Trench, Indian Ocean. Int J Syst Evol Microbiol 58:57–60. (PMID: 10.1099/ijs.0.64869-0)
Borsodi AK et al (2011) Bacillus alkalisediminis sp nov., an alkaliphilic and moderately halophilic bacterium isolated from sediment of extremely shallow soda ponds. Int J Syst Evol Microbiol 61:1880–1886. (PMID: 10.1099/ijs.0.019489-0)
Breed (1953) The Brevibacteriaceae fam. nov. of order Eubacteriales. Riass. Commun. VI Congr. Int. Microbiol. Roma:13–14.
Buck JD (1982) Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993. (PMID: 10.1128/aem.44.4.992-993.1982)
Chen P, Zhang L, Wang J, Ruan J, Han X, Huang Y (2016) Brevibacterium sediminis sp. nov., isolated from deep-sea sediments from the Carlsberg and Southwest Indian Ridges. Int J Syst Evol Microbiol 66:5268–5274. (PMID: 10.1099/ijsem.0.001506)
Choi EJ, Lee SH, Jung JY, Jeon CO (2013) Brevibacterium jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 63:3430–3436. (PMID: 10.1099/ijs.0.049197-0)
Collins MD, Farrow JAE, Goodfellow M, Minnikin DE (1983) Brevibacterium casei sp. nov. and Brevibacterium epidermidis sp. nov. Syst Appl Microbiol 4:388–395. (PMID: 10.1016/S0723-2020(83)80023-X)
Collins MD (2006) The genus Brevibacterium. In: Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E et al (eds) Prokaryotes. Springer, New York, pp 1013–1019. (PMID: 10.1007/0-387-30743-5_42)
Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376. (PMID: 10.1007/BF01734359)
Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416. (PMID: 10.2307/2412116)
Gonzalez C, Gutierrez C, Ramirez C (1978) Halobacterium vallismortis sp. nov. An amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24:710–715. (PMID: 10.1139/m78-119)
Hu HY, Lim BR, Goto N, Fujie K (2001) Analytical precision and repeatability of respiratory quinones for quantitative study of microbial community structure in environmental samples. J Microbiol Methods 47:17–24. (PMID: 10.1016/S0167-7012(01)00286-X)
Ivanova EP et al (2004) Brevibacterium celere sp. nov., isolated from degraded thallus of a brown alga. Int J Syst Evol Microbiol 54:2107–2111. (PMID: 10.1099/ijs.0.02867-0)
Jung MS et al (2018) Brevibacterium anseongense sp. nov., isolated from soil of ginseng field. J Microbiol 56:706–712. (PMID: 10.1007/s12275-018-8181-5)
Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120. (PMID: 10.1007/BF01731581)
Kodaka H, Armfield AY, Lombard GL, Dowell VRJ (1982) Practical procedure for demonstrating bacterial flagella. J Clin Microbiol 16:948–952. (PMID: 10.1128/jcm.16.5.948-952.1982)
Li R, Li Y, Kristiansen K, Wang J (2008) SOAP: short oligonucleotide alignment program. Bioinformatics 24:713–714. (PMID: 10.1093/bioinformatics/btn025)
Li R et al (2010) De novo assembly of human genomes with massively parallel short read sequencing. Genome Res 20:265–272. (PMID: 10.1101/gr.097261.109)
Richter M, Rossello-Mora R (2009) Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 106:19126–19131. (PMID: 10.1073/pnas.0906412106)
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425.
Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. MIDI Inc., Newark, DE, USA.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729. (PMID: 10.1093/molbev/mst197)
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res 25:4876–4882. (PMID: 10.1093/nar/25.24.4876)
Wayne LG (1988) Announcement of the Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Zentralblatt für Mikrobiologie 143:403–404. (PMID: 10.1016/S0232-4393(88)80035-0)
Xu P et al (2005) Naxibacter alkalitolerans gen. nov., sp nov., a novel member of the family “Oxalobacteraceae” isolated from China. Int J Syst Evol Microbiol 55:1149–1153. (PMID: 10.1099/ijs.0.63407-0)
Yoon S et al (2017) Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 67:1613–1617. (PMID: 10.1099/ijsem.0.001755)
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