Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Digital PCR for high sensitivity viral detection in false-negative SARS-CoV-2 patients.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : Nature Publishing Group, copyright 2011-
    • الموضوع:
      False Negative Reactions*; COVID-19/*diagnosis ; Real-Time Polymerase Chain Reaction/*methods ; SARS-CoV-2/*pathogenicity; Adult ; Aged ; COVID-19/diagnostic imaging ; COVID-19/genetics ; Diagnostic Tests, Routine/methods ; Female ; Humans ; Male ; Middle Aged ; SARS-CoV-2/genetics ; Sensitivity and Specificity ; Tomography, X-Ray Computed
    • نبذة مختصرة :
      Patients requiring diagnostic testing for coronavirus disease 2019 (COVID-19) are routinely assessed by reverse-transcription quantitative polymerase chain reaction (RT-qPCR) amplification of Sars-CoV-2 virus RNA extracted from oro/nasopharyngeal swabs. Despite the good specificity of the assays certified for SARS-CoV-2 molecular detection, and a theoretical sensitivity of few viral gene copies per reaction, a relatively high rate of false negatives continues to be reported. This is an important challenge in the management of patients on hospital admission and for correct monitoring of the infectivity after the acute phase. In the present report, we show that the use of digital PCR, a high sensitivity method to detect low amplicon numbers, allowed us to correctly detecting infection in swab material in a significant number of false negatives. We show that the implementation of digital PCR methods in the diagnostic assessment of COVID-19 could resolve, at least in part, this timely issue.
    • References:
      Mayo Clin Proc. 2020 Jun;95(6):1127-1129. (PMID: 32376102)
      ACS Sens. 2020 Aug 28;5(8):2283-2296. (PMID: 32627534)
      J Med Virol. 2020 Oct;92(10):1755-1756. (PMID: 32270882)
      Cell Rep. 2020 Sep 22;32(12):108175. (PMID: 32946807)
      Nature. 2020 Jun;582(7813):557-560. (PMID: 32340022)
      Eur Radiol. 2020 Aug;30(8):4381-4389. (PMID: 32193638)
      Emerg Microbes Infect. 2020 Dec;9(1):1259-1268. (PMID: 32438868)
      Eur J Radiol. 2020 May;126:108961. (PMID: 32229322)
      Int J Infect Dis. 2020 Jun;95:304-307. (PMID: 32344011)
      Int J Mol Med. 2020 Sep;46(3):957-964. (PMID: 32705153)
      ACS Nano. 2020 Apr 28;14(4):3822-3835. (PMID: 32223179)
      Cell. 2020 May 14;181(4):894-904.e9. (PMID: 32275855)
      JAMA. 2020 Aug 25;324(8):782-793. (PMID: 32648899)
      JAMA. 2020 Apr 21;323(15):1437-1438. (PMID: 32150622)
      Nat Biotechnol. 2020 Oct;38(10):1168-1173. (PMID: 32733106)
      EBioMedicine. 2020 Sep;59:102903. (PMID: 32718896)
      RNA. 2020 Jul;26(7):771-783. (PMID: 32358057)
      medRxiv. 2020 Jun 14;:. (PMID: 32587981)
      Emerg Microbes Infect. 2020 Dec;9(1):747-756. (PMID: 32196430)
    • الموضوع:
      Date Created: 20210223 Date Completed: 20210308 Latest Revision: 20231104
    • الموضوع:
      20240829
    • الرقم المعرف:
      PMC7900100
    • الرقم المعرف:
      10.1038/s41598-021-83723-x
    • الرقم المعرف:
      33619321