Antitumor Effect of the Ethanolic Extract from Seeds of Euphorbia lathyris in Colorectal Cancer.

Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101521595 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6643 (Electronic) Linking ISSN: 20726643 NLM ISO Abbreviation: Nutrients Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI Publishing

Cell Proliferation/*drug effects ; Colonic Neoplasms/*pathology ; Euphorbia/*chemistry ; Plant Extracts/*pharmacology ; Seeds/*chemistry; Adenocarcinoma/pathology ; Antineoplastic Agents, Phytogenic ; Antioxidants/analysis ; Apoptosis/drug effects ; Cell Line, Tumor ; Colonic Neoplasms/drug therapy ; Ethanol ; Glioblastoma/pathology ; Human Umbilical Vein Endothelial Cells ; Humans ; Neovascularization, Physiologic/drug effects ; Plant Extracts/therapeutic use ; Polyphenols/analysis

The seeds of Euphorbia lathyris have been used in traditional medicine to treat various medical conditions. However, neither all of their active biocompounds nor the molecular mechanisms underlying their therapeutic effects have been described. A new ethanolic extract of defatted flour from mature seeds of Euphorbia lathyris showed a high total polyphenol content and significant antioxidant activity. Chromatographic analysis showed that esculetin, euphorbetin, gaultherin, and kaempferol-3-rutinoside were the most abundant polyphenolic bioactive compounds. Antiproliferative assays showed a high and selective antitumor activity against colon cancer cell lines (T84 and HCT-15). In addition, a significant antiproliferative activity against glioblastoma multiforme cells was also demonstrated. Its mechanism of action to induce cell death was mediated by the overexpression of caspases 9, 3, and 8, and by activation of autophagy. Interestingly, a reduction in the migration capacity of colon cancer cells and a significant antiangiogenic effect on human umbilical vein endothelial cells were also demonstrated. Finally, the extract significantly reduced the subpopulations of cancer stem cells. This extract could be the basis to develop new therapeutic strategies for the treatment of colon cancer, although further experiments will be necessary to determine its in vivo effects.

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PSE/17/002 Universidad de Granada; RTC-2017-6540-1 Ministerio de Ciencia e Innovación

Keywords: Euphorbia lathyris; antiangiogenic effect; apoptosis; cancer stem cells; colon cancer; ethanolic extract

0 (Antineoplastic Agents, Phytogenic)
0 (Antioxidants)
0 (Plant Extracts)
0 (Polyphenols)
3K9958V90M (Ethanol)

Date Created: 20210212 Date Completed: 20210902 Latest Revision: 20210902

20240628

PMC7915714

10.3390/nu13020566

33572111