Vancomycin-resistant Enterococcus faecium in Algeria: phenotypic and genotypic characterization of clinical isolates.

Publisher: Open Learning on Enteric Pathogens Country of Publication: Italy NLM ID: 101305410 Publication Model: Electronic Cited Medium: Internet ISSN: 1972-2680 (Electronic) Linking ISSN: 19722680 NLM ISO Abbreviation: J Infect Dev Ctries Subsets: MEDLINE

Original Publication: [Italy?] : Open Learning on Enteric Pathogens

Anti-Bacterial Agents/*pharmacology ; Enterococcus faecium/*drug effects ; Enterococcus faecium/*genetics ; Gram-Positive Bacterial Infections/*microbiology ; Vancomycin/*pharmacology ; Vancomycin-Resistant Enterococci/*drug effects ; Vancomycin-Resistant Enterococci/*genetics; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Algeria/epidemiology ; Child ; Child, Preschool ; Cross Infection/microbiology ; Daptomycin/pharmacology ; Drug Resistance, Multiple, Bacterial ; Enterococcus faecium/isolation & purification ; Female ; Genotype ; Hospitals ; Humans ; Infant ; Male ; Microbial Sensitivity Tests ; Middle Aged ; Molecular Epidemiology ; Multilocus Sequence Typing ; Teicoplanin/pharmacology ; Tigecycline/pharmacology ; Vancomycin Resistance ; Vancomycin-Resistant Enterococci/isolation & purification ; Virulence Factors ; Young Adult

Competing Interests: No Conflict of Interest is declared
Introduction: vancomycin-resistant Enterococcus faecium (VREfm) is a major public health problem worldwide. The aim of our study was to determine the microbiological, epidemiological and molecular characteristics of VREfm isolated in north-central, eastern and western Algeria.
Methodology: a collection of 48 VREfm isolated from September 2010 to April 2017 in several Algerian hospitals were studied. Minimum inhibitory concentrations (MICs) were determined by E-test method according to CLSI guidelines. the detection of van genotype of all strains was performed by PCR. Clonal relationship of five VREfm targeted by region were characterized using multilocus sequence typing (MLST).
Results: All isolates have multidrug-resistance (MDR) and were resistant to at least five classes of antibiotics; however, all were susceptible to tigecycline and daptomycin with MIC50 at 0.094 µg/mL and 2 µg/mL respectively. All strains belonged to vanA genotype and have high level of resistance to vancomycin and teicoplanin. MLST revealed two sequence types (STs): ST80 (from the four regions of Algeria) and ST789, both belonging to the former hospital-adapted clonal complex CC17.
Conclusions: the alarming dissemination of MDR E. faecium vanA and the ST80 in several regions of Algeria suggest a clonal spread of VREfm strains, which urgently require implementation of adequate infection control measures.
(Copyright (c) 2021 Nabila Benamrouche, Badia Guettou, Fatma Zohra Henniche, Farida Assaous, Houcine Laouar, Hanifa Ziane, Fazia Djennane, Djamal Tiouit, Chafia Bentchouala, Ferroudja Yamouni, Kheira Rahal, Hassiba Tali Maamar.)

Keywords: Algeria; E. faecium; Enterococcus faecium; MLST; antimicrobial susceptibility; vanA; vancomycin resistant

0 (Anti-Bacterial Agents)
0 (Virulence Factors)
61036-62-2 (Teicoplanin)
6Q205EH1VU (Vancomycin)
70JE2N95KR (Tigecycline)
NWQ5N31VKK (Daptomycin)

Date Created: 20210211 Date Completed: 20211025 Latest Revision: 20211025

20250114

10.3855/jidc.12482

33571151