Response differences of HepG2 and Primary Mouse Hepatocytes to morphological changes in electrospun PCL scaffolds.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Cytochrome P-450 CYP1A2/*genetics ; Liver/*metabolism ; Liver Diseases/*therapy ; Tissue Scaffolds/*chemistry; Animals ; Biomimetics ; Cell Proliferation/genetics ; Gene Expression Regulation ; Hep G2 Cells ; Hepatocytes/metabolism ; Hepatocytes/pathology ; Humans ; Liver/growth & development ; Liver/pathology ; Liver Diseases/genetics ; Liver Diseases/pathology ; Mice ; Tissue Engineering/methods

Liver disease cases are rapidly expanding across the globe and the only effective cure for end-stage disease is a transplant. Transplant procedures are costly and current supply of donor livers does not satisfy demand. Potential drug treatments and regenerative therapies that are being developed to tackle these pressing issues require effective in-vitro culture platforms. Electrospun scaffolds provide bio-mimetic structures upon which cells are cultured to regulate function in-vitro. This study aims to shed light on the effects of electrospun PCL morphology on the culture of an immortalised hepatic cell line and mouse primary hepatocytes. Each cell type was cultured on large 4-5 µm fibres and small 1-2 µm fibres with random, aligned and highly porous cryogenically spun configurations. Cell attachment, proliferation, morphology and functional protein and gene expression was analysed. Results show that fibre morphology has a measurable influence on cellular morphology and function, with the alteration of key functional markers such as CYP1A2 expression.

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EC 1.14.14.1 (Cytochrome P-450 CYP1A2)

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