Ligand-dependent tRNA processing by a rationally designed RNase P riboswitch.

Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE

Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.

RNA Processing, Post-Transcriptional* ; Riboswitch*; RNA, Transfer/*metabolism ; Ribonuclease P/*metabolism; Computer Simulation ; Escherichia coli/genetics ; Ligands ; RNA, Transfer/chemistry ; Ribonuclease P/chemistry ; Thermodynamics

We describe a synthetic riboswitch element that implements a regulatory principle which directly addresses an essential tRNA maturation step. Constructed using a rational in silico design approach, this riboswitch regulates RNase P-catalyzed tRNA 5'-processing by either sequestering or exposing the single-stranded 5'-leader region of the tRNA precursor in response to a ligand. A single base pair in the 5'-leader defines the regulatory potential of the riboswitch both in vitro and in vivo. Our data provide proof for prior postulates on the importance of the structure of the leader region for tRNA maturation. We demonstrate that computational predictions of ligand-dependent structural rearrangements can address individual maturation steps of stable non-coding RNAs, thus making them amenable as promising target for regulatory devices that can be used as functional building blocks in synthetic biology.
(© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.)

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0 (Ligands)
0 (Riboswitch)
9014-25-9 (RNA, Transfer)
EC 3.1.26.5 (Ribonuclease P)

Date Created: 20210120 Date Completed: 20210303 Latest Revision: 20210303

20240829

PMC7897497

10.1093/nar/gkaa1282

33469651