The attachment process and physiological properties of Escherichia coli O157:H7 on quartz.

Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180 (Electronic) Linking ISSN: 14712180 NLM ISO Abbreviation: BMC Microbiol Subsets: MEDLINE

Original Publication: London : BioMed Central, [2001-

Bacterial Adhesion*; Escherichia coli O157/*physiology ; Quartz/*metabolism; Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Chemotaxis ; Drug Resistance, Bacterial ; Escherichia coli O157/drug effects ; Escherichia coli O157/genetics ; Escherichia coli O157/metabolism ; Gene Expression Profiling ; Proteomics ; Signal Transduction ; Stress, Physiological

Background: Manure application and sewage irrigation release many intestinal pathogens into the soil. After being introduced into the soil matrix, pathogens are commonly found to attach to soil minerals. Although the survival of mineral-associated Escherichia coli O157:H7 has been studied, a comprehensive understanding of the attachment process and physiological properties after attachment is still lacking.
Results: In this study, planktonic and attached Escherichia coli O157:H7 cells on quartz were investigated using RNA sequencing (RNA-seq) and the isobaric tagging for relative and absolute quantitation (iTRAQ) proteomic method. Based on the transcriptomic and proteomic analyses and gene knockouts, functional two-component system pathways were required for efficient attachment; chemotaxis and the Rcs system were identified to play determinant roles in E. coli O157:H7 attachment on quartz. After attachment, the pyruvate catabolic pathway shifted from the tricarboxylic acid (TCA) cycle toward the fermentative route. The survival rate of attached E. coli O157:H7 increased more than 10-fold under penicillin and vancomycin stress and doubled under alkaline pH and ferric iron stress.
Conclusions: These results contribute to the understanding of the roles of chemotaxis and the Rcs system in the attachment process of pathogens and indicate that the attachment of pathogens to minerals significantly elevates their resistance to antibiotics and environmental stress, which may pose a potential threat to public health.

BMC Genomics. 2014 Jun 24;15:515. (PMID: 24962434)
BMC Microbiol. 2010 Feb 23;10:57. (PMID: 20175929)
Int J Food Microbiol. 2016 Jun 16;227:17-21. (PMID: 27043385)
Colloids Surf B Biointerfaces. 2019 Sep 1;181:215-225. (PMID: 31146245)
J Bacteriol. 1998 May;180(9):2442-9. (PMID: 9573197)
Environ Sci Technol. 2013 Jun 18;47(12):6223-30. (PMID: 23692120)
Mol Syst Biol. 2009;5:335. (PMID: 20029369)
EMBO J. 2010 Aug 18;29(16):2724-33. (PMID: 20717142)
Colloids Surf B Biointerfaces. 2016 Mar 1;139:148-55. (PMID: 26705829)
Int J Food Microbiol. 2017 Nov 2;260:11-16. (PMID: 28843119)
Ann Ist Super Sanita. 2015;51(1):62-6. (PMID: 25857386)
BMC Microbiol. 2017 Mar 8;17(1):56. (PMID: 28274217)
mBio. 2016 Aug 30;7(4):. (PMID: 27578754)
Appl Microbiol Biotechnol. 2012 Feb;93(3):983-92. (PMID: 22183085)
J Bacteriol. 2001 Dec;183(24):7213-23. (PMID: 11717281)
Protein Sci. 2019 Nov;28(11):1947-1951. (PMID: 31441146)
Appl Environ Microbiol. 2002 May;68(5):2605-9. (PMID: 11976144)
Mol Microbiol. 2003 Aug;49(3):823-32. (PMID: 12864862)
Colloids Surf B Biointerfaces. 2016 Oct 1;146:70-83. (PMID: 27259161)
Appl Environ Microbiol. 2012 Mar;78(6):1752-64. (PMID: 22247152)
Colloids Surf B Biointerfaces. 2018 Dec 1;172:197-206. (PMID: 30170233)
J Biosci Bioeng. 2002;93(6):543-9. (PMID: 16233247)
BMC Microbiol. 2005 Jun 17;5:36. (PMID: 15963233)
J Mol Biol. 2006 Jan 27;355(4):798-808. (PMID: 16337229)
Colloids Surf B Biointerfaces. 2018 Jul 1;167:1-7. (PMID: 29625418)
Appl Environ Microbiol. 2000 Mar;66(3):877-83. (PMID: 10698745)
J Exp Bot. 2015 Jul;66(13):3699-715. (PMID: 25911739)
Mol Cell Proteomics. 2013 Aug;12(8):2266-77. (PMID: 23665590)
Int J Food Microbiol. 2010 Jan 31;137(1):13-21. (PMID: 19853945)
BMC Microbiol. 2017 Jan 25;17(1):24. (PMID: 28122502)
Mol Cell Probes. 2004 Apr;18(2):123-32. (PMID: 15051122)
Environ Sci Technol. 2013 Feb 19;47(4):1896-903. (PMID: 23346967)
Mol Cell Proteomics. 2013 May;12(5):1363-76. (PMID: 23408684)
Environ Pollut. 2019 Sep;252(Pt B):1078-1086. (PMID: 31252105)
BMC Genomics. 2014 May 09;15:353. (PMID: 24885796)
Nat Rev Microbiol. 2011 Mar;9(3):153-65. (PMID: 21283116)
J Hazard Mater. 2015 Oct 15;296:30-36. (PMID: 25910457)
Acta Virol. 2016;60(3):328-32. (PMID: 27640443)
J Proteome Res. 2014 Feb 7;13(2):735-51. (PMID: 24428189)
J Bacteriol. 2012 Feb;194(3):669-76. (PMID: 22139504)
BMC Microbiol. 2012 Nov 15;12:261. (PMID: 23153211)
Crit Rev Microbiol. 2015;41(3):273-94. (PMID: 24083946)
Environ Microbiol. 2014 Jan;16(1):291-303. (PMID: 24148021)
FEMS Microbiol Lett. 2012 Jan;326(1):2-11. (PMID: 22092948)
Int J Environ Res Public Health. 2017 Oct 18;14(10):. (PMID: 29057845)
Microb Cell. 2016 Sep 19;3(10):522-528. (PMID: 28357320)
Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2287-92. (PMID: 11830644)
Appl Environ Microbiol. 2005 May;71(5):2226-31. (PMID: 15870304)
Colloids Surf B Biointerfaces. 2017 Jun 1;154:82-88. (PMID: 28324690)
Trends Microbiol. 2014 Sep;22(9):517-27. (PMID: 24894628)
Colloids Surf B Biointerfaces. 2018 Mar 1;163:201-208. (PMID: 29304434)
Environ Pollut. 2018 Dec;243(Pt B):953-960. (PMID: 30245456)
BMC Microbiol. 2019 Sep 12;19(1):215. (PMID: 31510932)
Microbiology (Reading). 2015 Apr;161(Pt 4):903-13. (PMID: 25667010)

Keywords: Chemotaxis; Escherichia coli O157:H7; Fermentative route; Rcs system; Stress susceptibility

0 (Anti-Bacterial Agents)
0 (Bacterial Proteins)
14808-60-7 (Quartz)

Date Created: 20201120 Date Completed: 20210803 Latest Revision: 20210803

20250114

PMC7677791

10.1186/s12866-020-02043-8

33213384