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Orexin system is expressed in avian liver and regulates hepatic lipogenesis via ERK1/2 activation.

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  • معلومة اضافية
    • المصدر:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : Nature Publishing Group, copyright 2011-
    • الموضوع:
    • نبذة مختصرة :
      Orexins are originally characterized as orexigenic hypothalamic neuropeptides in mammals. Subsequent studies found orexin to be expressed and perform pleiotropic functions in multiple tissues in mammals. In avian (non-mammalian) species, however, orexin seemed to not affect feeding behavior and its physiological roles are poorly understood. Here, we provide evidence that orexin and its related receptors are expressed in chicken hepatocytes. Double immunofluorescence staining showed that orexin is localized in the ER, Golgi, and in the lysosomes in LMH cells. Brefeldin A treatment reduced orexin levels in the culture media, but increased it in the cell lysates. Administration of recombinant orexins upregulated the expression of orexin system in the liver of 9-day old chicks, but did not affect feed intake. Recombinant orexins increased fatty acid synthase (FASN) protein levels in chicken liver, activated acetyl-CoA carboxylase (ACCα), and increased FASN, ATP citrate lyase(ACLY), and malic enzyme (ME) protein expression in LMH cells. Blockade ERK1/2 activation by PD98059 attenuated these stimulating effects of orexin on lipogenic factors. Overexpression of ERK1/2 increased the expression of lipogenic genes, and orexin treatment induced the phosphorylated levels of ERK1/2 Thr202/Tyr204 , but not that of p38 Thr180/Tyr182 or JNK1/2 Thr183/Tyr185 in chicken liver and LMH cells. Taken together, this is the first report evidencing that orexin is expressed and secreted from chicken hepatocytes, and that orexin induced hepatic lipogenesis via activation of ERK1/2 signaling pathway.
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    • الرقم المعرف:
      0 (Orexins)
      0 (Protein Synthesis Inhibitors)
      20350-15-6 (Brefeldin A)
      EC 1.1.1.37 (Malate Dehydrogenase)
      EC 1.1.1.39 (malate dehydrogenase (decarboxylating))
      EC 2.3.1.85 (Fatty Acid Synthases)
      EC 6.4.1.2 (Acetyl-CoA Carboxylase)
    • الموضوع:
      Date Created: 20201106 Date Completed: 20210309 Latest Revision: 20210309
    • الموضوع:
      20240829
    • الرقم المعرف:
      PMC7645691
    • الرقم المعرف:
      10.1038/s41598-020-76329-2
    • الرقم المعرف:
      33154530