Nucleo-plastidic PAP8/pTAC6 couples chloroplast formation with photomorphogenesis.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 8208664 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-2075 (Electronic) Linking ISSN: 02614189 NLM ISO Abbreviation: EMBO J Subsets: MEDLINE

Publication: 2024- : [London] : Nature Publishing Group
Original Publication: Eynsham, Oxford, England : Published for the European Molecular Biology Organization by IRL Press, [c1982-

Acid Phosphatase/*metabolism ; Arabidopsis/*genetics ; Arabidopsis/*metabolism ; Arabidopsis Proteins/*metabolism ; Chloroplasts/*metabolism ; Morphogenesis/*physiology ; Plastids/*genetics ; Plastids/*metabolism; Acid Phosphatase/genetics ; Arabidopsis Proteins/genetics ; Cell Nucleus/metabolism ; Chloroplasts/genetics ; DNA-Directed RNA Polymerases/metabolism ; Gene Expression Regulation, Plant ; Light ; Organelle Biogenesis ; Phytochrome/metabolism ; Plants, Genetically Modified ; Signal Transduction ; Transcription Factors ; Transcription, Genetic

The initial greening of angiosperms involves light activation of photoreceptors that trigger photomorphogenesis, followed by the development of chloroplasts. In these semi-autonomous organelles, construction of the photosynthetic apparatus depends on the coordination of nuclear and plastid gene expression. Here, we show that the expression of PAP8, an essential subunit of the plastid-encoded RNA polymerase (PEP) in Arabidopsis thaliana, is under the control of a regulatory element recognized by the photomorphogenic factor HY5. PAP8 protein is localized and active in both plastids and the nucleus, and particularly required for the formation of late photobodies. In the pap8 albino mutant, phytochrome-mediated signalling is altered, degradation of the chloroplast development repressors PIF1/PIF3 is disrupted, HY5 is not stabilized, and the expression of the photomorphogenesis regulator GLK1 is impaired. PAP8 translocates into plastids via its targeting pre-sequence, interacts with the PEP and eventually reaches the nucleus, where it can interact with another PEP subunit pTAC12/HMR/PAP5. Since PAP8 is required for the phytochrome B-mediated signalling cascade and the reshaping of the PEP activity, it may coordinate nuclear gene expression with PEP-driven chloroplastic gene expression during chloroplast biogenesis.
(© 2020 The Authors.)

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Université Grenoble Alpes (UGA); AGIR; ANR-10-INSB-05-02 Agence Nationale de la Recherche (ANR); ANR-17-EURE-0003 Agence Nationale de la Recherche (ANR); ANR-PEPRegulChloro3D Agence Nationale de la Recherche (ANR); PF323-5 Deutsche Forschungsgemeinschaft (DFG)

Keywords: Arabidopsis PEP/PAPs; biogenesis; chloroplast; photobodies; photomorphogenesis

0 (Arabidopsis Proteins)
0 (GLK1 protein, Arabidopsis)
0 (HMR protein, Arabidopsis)
0 (Transcription Factors)
11121-56-5 (Phytochrome)
EC 2.7.7.6 (DNA-Directed RNA Polymerases)
EC 3.1.3.- (purple acid phosphatase)
EC 3.1.3.2 (Acid Phosphatase)

Date Created: 20201001 Date Completed: 20210414 Latest Revision: 20211117

20250114

PMC7667882

10.15252/embj.2020104941

33001465