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Overexpression of miR-29a-3p Suppresses Proliferation, Migration, and Invasion of Vascular Smooth Muscle Cells in Atherosclerosis via Targeting TNFRSF1A.
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- المؤلفون: You L;You L;You L; Chen H; Chen H; Xu L; Xu L; Li X; Li X
- المصدر:
BioMed research international [Biomed Res Int] 2020 Sep 04; Vol. 2020, pp. 9627974. Date of Electronic Publication: 2020 Sep 04 (Print Publication: 2020).
- نوع النشر :
Journal Article
- اللغة:
English
- معلومة اضافية
- المصدر:
Publisher: Hindawi Pub. Co Country of Publication: United States NLM ID: 101600173 Publication Model: eCollection Cited Medium: Internet ISSN: 2314-6141 (Electronic) NLM ISO Abbreviation: Biomed Res Int Subsets: MEDLINE
- بيانات النشر:
Original Publication: New York, NY : Hindawi Pub. Co.
- الموضوع:
- نبذة مختصرة :
Objective: Increasing evidence highlights the significance of microRNAs (miRNAs) in the progression of atherosclerosis (AS). Our aim was to probe out the role and regulatory mechanism of miR-29a-3p in AS.
Methods: An in vivo model of AS was conducted by high-fat diet ApoE -/- mice. Oxidized low-density lipoprotein- (ox-LDL-) exposed vascular smooth muscle cells (VSMCs) were utilized as an in vitro of AS. Serum levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were detected. Hematoxylin and eosin (H&E) and Masson's staining was presented to investigate the pathological changes. miR-29a-3p and TNFRSF1A expression was detected by RT-qPCR. Proliferative, migrated, and invaded abilities of VSMCs were determined via a series of assays. The interaction between miR-29a-3p and TNFRSF1A was verified through luciferase reporter assay.
Results: Upregulated miR-29a-3p and downregulated TNFRSF1A were found both in vitro and in vivo models of AS. miR-29a-3p mimic distinctly decreased the serum levels of TC, TG, and LDL-C and increased serum HDL-C levels. Moreover, its overexpression could ameliorate plaque formation of AS mice. In ox-LDL-induced VSMCs, miR-29a-3p overexpression notably decreased cell proliferation, migration, and invasion, which was reversed by TNFRSF1A overexpression. Also, miR-29a-3p could directly target the 3'UTR of TNFRSF1A.
Conclusion: miR-29a-3p overexpression ameliorated plaque formation of AS and suppressed proliferation, migration, and invasion of ox-LDL-induced VSMCs via TNFRSF1A, which offered novel insights into the progression of AS.
Competing Interests: The authors declare no conflicts of interest.
(Copyright © 2020 Liyi You et al.)
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- الرقم المعرف:
0 (3' Untranslated Regions)
0 (Lipoproteins, LDL)
0 (MIRN29 microRNA, mouse)
0 (MicroRNAs)
0 (Receptors, Tumor Necrosis Factor, Type I)
0 (Tnfrsf1a protein, mouse)
0 (oxidized low density lipoprotein)
- الموضوع:
Date Created: 20200923 Date Completed: 20210426 Latest Revision: 20220417
- الموضوع:
20240829
- الرقم المعرف:
PMC7492923
- الرقم المعرف:
10.1155/2020/9627974
- الرقم المعرف:
32964047
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