Overexpression of miR-29a-3p Suppresses Proliferation, Migration, and Invasion of Vascular Smooth Muscle Cells in Atherosclerosis via Targeting TNFRSF1A.

Publisher: Hindawi Pub. Co Country of Publication: United States NLM ID: 101600173 Publication Model: eCollection Cited Medium: Internet ISSN: 2314-6141 (Electronic) NLM ISO Abbreviation: Biomed Res Int Subsets: MEDLINE

Original Publication: New York, NY : Hindawi Pub. Co.

Atherosclerosis/*genetics ; Atherosclerosis/*pathology ; Cell Movement/*genetics ; Cell Proliferation/*genetics ; MicroRNAs/*genetics ; Muscle, Smooth, Vascular/*pathology ; Receptors, Tumor Necrosis Factor, Type I/*genetics; 3' Untranslated Regions/genetics ; Animals ; Cells, Cultured ; Down-Regulation/genetics ; Lipoproteins, LDL/genetics ; Male ; Mice ; Myocytes, Smooth Muscle/pathology ; Signal Transduction/genetics ; Up-Regulation/genetics

Objective: Increasing evidence highlights the significance of microRNAs (miRNAs) in the progression of atherosclerosis (AS). Our aim was to probe out the role and regulatory mechanism of miR-29a-3p in AS.
Methods: An in vivo model of AS was conducted by high-fat diet ApoE ^-/- mice. Oxidized low-density lipoprotein- (ox-LDL-) exposed vascular smooth muscle cells (VSMCs) were utilized as an in vitro of AS. Serum levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were detected. Hematoxylin and eosin (H&E) and Masson's staining was presented to investigate the pathological changes. miR-29a-3p and TNFRSF1A expression was detected by RT-qPCR. Proliferative, migrated, and invaded abilities of VSMCs were determined via a series of assays. The interaction between miR-29a-3p and TNFRSF1A was verified through luciferase reporter assay.
Results: Upregulated miR-29a-3p and downregulated TNFRSF1A were found both in vitro and in vivo models of AS. miR-29a-3p mimic distinctly decreased the serum levels of TC, TG, and LDL-C and increased serum HDL-C levels. Moreover, its overexpression could ameliorate plaque formation of AS mice. In ox-LDL-induced VSMCs, miR-29a-3p overexpression notably decreased cell proliferation, migration, and invasion, which was reversed by TNFRSF1A overexpression. Also, miR-29a-3p could directly target the 3'UTR of TNFRSF1A.
Conclusion: miR-29a-3p overexpression ameliorated plaque formation of AS and suppressed proliferation, migration, and invasion of ox-LDL-induced VSMCs via TNFRSF1A, which offered novel insights into the progression of AS.
Competing Interests: The authors declare no conflicts of interest.
(Copyright © 2020 Liyi You et al.)

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0 (3' Untranslated Regions)
0 (Lipoproteins, LDL)
0 (MIRN29 microRNA, mouse)
0 (MicroRNAs)
0 (Receptors, Tumor Necrosis Factor, Type I)
0 (Tnfrsf1a protein, mouse)
0 (oxidized low density lipoprotein)

Date Created: 20200923 Date Completed: 20210426 Latest Revision: 20220417

20240829

PMC7492923

10.1155/2020/9627974

32964047