Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c1995-

Biomarkers* ; Biosensing Techniques*; Magnetite Nanoparticles/*chemistry ; Saliva, Artificial/*analysis; Humans ; Magnetite Nanoparticles/ultrastructure ; Models, Theoretical ; Molecular Structure ; Particle Size

Magnetic nanoparticles (MNPs) were synthesized using the colloidal co-precipitation method and further coated with silica using the Stöber process. These were functionalized with carboxylic and amine functionalities for further covalent immobilization of antibodies on these MNPs. The procedure for covalent immobilization of antibodies on MNPs was developed using 1-ethyl-3-(dimethylaminopropyl)carbodiimide (EDC) and N -hydroxysuccinimide (NHS). The evaluation of the efficiency of the coupling reaction was carried out by UV-vis spectrophotometry. The developed antibodies coupled to MNPs were tested for the pre-concentration of two biomarkers tumor necrosis factor alpha (TNF-α) and Interleukin-10 (IL-10). Both biomarkers were assessed in the matrix based on phosphate-buffered saline solution (PBS) and artificial saliva (AS) to carry out the demonstration of the format assay. Supernatants were used to determine the number of free biomarkers for both studies. Reduction of the nonspecific saliva protein adsorption on the surface of the complex antibodies-MNPs to levels low enough to allow the detection of biomarkers in complex media has been achieved.

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Horizon 2020 KardiaTool under grant agreement No 768686 European Union; CAMPUS FRANCE program grant agreement PHC PROCOPE 40544QH; CTQ2016-75150-R Spanish Ministerio de Economia y Competitividad; 2014/SGR/149 Generalitat de Catalunya

Keywords: IL-10; TNF-α; immobilization of antibodies; magnetic nanoparticles; pre-concentration of antigens; saliva matrix

0 (Biomarkers)
0 (Magnetite Nanoparticles)
0 (Saliva, Artificial)

Date Created: 20200904 Date Completed: 20210316 Latest Revision: 20210316

20221213

PMC7504804

10.3390/molecules25173968

32878151