Serotypes, antibiotic resistance, and virulence genes of Salmonella in children with diarrhea.

Publisher: Wiley Country of Publication: United States NLM ID: 8801384 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-2825 (Electronic) Linking ISSN: 08878013 NLM ISO Abbreviation: J Clin Lab Anal Subsets: MEDLINE

Publication: Hoboken, N.J. : Wiley
Original Publication: New York : Alan R. Liss, Inc., c1987-

Salmonella enteritidis*/drug effects ; Salmonella enteritidis*/genetics ; Salmonella enteritidis*/pathogenicity ; Salmonella typhimurium*/drug effects ; Salmonella typhimurium*/genetics ; Salmonella typhimurium*/pathogenicity; Diarrhea/*microbiology ; Drug Resistance, Bacterial/*genetics ; Salmonella Infections/*microbiology; Anti-Bacterial Agents/pharmacology ; Child ; China ; Genes, Bacterial/genetics ; Humans ; Microbial Sensitivity Tests ; Serogroup ; Virulence Factors/genetics

Background: Salmonella is an important foodborne pathogen that causes acute diarrhea in humans worldwide. This study analyzed the relationships of serotypes and antibiotic resistance with virulence genes of Salmonella isolated from children with salmonellosis.
Methods: Serological typing was performed using the slide-agglutination method. The Kirby-Bauer disk diffusion method was used to test antibiotic susceptibility. Twenty virulence genes were detected by PCR.
Results: Salmonella Typhimurium (21 isolates, 34.43%) and S Enteritidis (12 isolates, 19.67%) were the predominant species among the 61 isolates. Ampicillin resistance was most common (63.93%), and among the cephalosporins, resistance was most often found to cefotaxime, a third-generation cephalosporin (19.67%). Among the 20 virulence genes, prgH, ssrB, and pagC were detected in all Salmonella isolates. In S Typhimurium, the detection rates of hilA, sipB, marT, mgtC, sopB, pagN, nlpI, bapA, oafA, and tolC were high. In S Enteritidis, the detection rates of icmF, spvB, spvR, and pefA were high. Nitrofurantoin resistance was negatively correlated with the virulence gene bapA (P = .005) and was positively correlated with icmF, spvB, spvR, and pefA (P = .012, .008, .002, and .005, respectively), The P values between all other virulence genes and antibiotic resistance were >.05.
Conclusion: Salmonella Typhimurium and S Enteritidis were the main serotypes in children with diarrhea in Hangzhou, China. Salmonella exhibited a high level of resistance to common antibiotics, and a high rate of bacteria carrying virulence genes was observed. However, no significant correlation was found between virulence genes and resistance to common antibiotics.
(© 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.)

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Keywords: Salmonella; antibiotic resistance; children; serotype; virulence genes

0 (Anti-Bacterial Agents)
0 (Virulence Factors)

Date Created: 20200816 Date Completed: 20211018 Latest Revision: 20221005

20240829

PMC7755775

10.1002/jcla.23525

32797660