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The soluble VEGF receptor sFlt-1 contributes to endothelial dysfunction in IgA nephropathy.

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اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: San Francisco, CA : Public Library of Science
    • الموضوع:
      Endothelium, Vascular/*physiopathology ; Glomerulonephritis, IGA/*physiopathology ; Vascular Endothelial Growth Factor Receptor-1/*physiology; Adult ; Apoptosis/drug effects ; Apoptosis/physiology ; Apoptosis Regulatory Proteins/physiology ; Caspase 9/drug effects ; Caspase Inhibitors/pharmacology ; Endothelium, Vascular/drug effects ; Endothelium, Vascular/pathology ; Female ; Glomerulonephritis, IGA/blood ; Glomerulonephritis, IGA/pathology ; Human Umbilical Vein Endothelial Cells ; Humans ; Immunoglobulin A/blood ; Immunoglobulin G/blood ; Male ; Middle Aged ; Mitochondria/drug effects ; Mitochondria/physiology ; Oligopeptides/pharmacology ; Vascular Endothelial Growth Factor Receptor-1/blood ; Young Adult
    • نبذة مختصرة :
      Endothelial injury is a common manifestation in IgA nephropathy (IgAN). After the previous identification of the upregulated soluble fms-like tyrosine kinase-1 (sFlt-1) correlated with endothelial injury in IgAN, in the present study, we further explored the role of sFlt-1 in endothelial injury in IgAN. We enrolled 72 patients with IgAN and detected the sFlt-1 levels. The polymeric IgA1 (pIgA1) complexes were isolated from the pooled plasma samples of another 10 patients with IgAN. Apoptosis proteins were detected in cultured human umbilical vein endothelial cells (HUVECs) with the stimulation of recombinant sFlt-1 or the caspase-9 inhibitor Z-LEHD-FMK. We identified there were positive correlations between sFlt-1 and IgA-IgG complex as well as vWF levels in patients with IgAN. The sFlt-1 levels in HUVECs were significantly upregulated by pIgA1 complex derived from IgAN patients in a concentration-dependent manner. The proliferation ability of HUVECs was damaged when stimulated with sFlt-1 protein in a time- and dose- dependent manner. And the apoptosis rate was up-regulated significantly as the stimulation concentrations of sFlt-1 increased. We found sFlt-1 challenge could significantly increase the expression of vWF. In addition, sFlt-1 increased the levels of caspase-9, caspase-3, Bax and mitochondrial membrane potential; facilitated the release of cytochrome C from mitochondria to cytoplasma. In contrast, Z-LEHD-FMK attenuated high sFlt-1-induced HUVECs apoptosis. In conclusion, our study demonstrated that sFlt-1 expression was up-regulated by the challenge of pIgA1 complex derived from patients with IgAN. Furthermore, increased sFlt-1 facilitated human umbilical vein endothelial cells apoptosis via the mitochondrial-dependent pathway.
      Competing Interests: The authors have declared that no competing interests exist.
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    • الرقم المعرف:
      0 (Apoptosis Regulatory Proteins)
      0 (Caspase Inhibitors)
      0 (Immunoglobulin A)
      0 (Immunoglobulin G)
      0 (Oligopeptides)
      0 (benzyloxycarbonyl-leucyl-glutamyl-histidyl-aspartic acid fluoromethyl ketone)
      0 (polymeric IgA)
      EC 2.7.10.1 (FLT1 protein, human)
      EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-1)
      EC 3.4.22.- (CASP9 protein, human)
      EC 3.4.22.- (Caspase 9)
    • الموضوع:
      Date Created: 20200814 Date Completed: 20201001 Latest Revision: 20201001
    • الموضوع:
      20231215
    • الرقم المعرف:
      PMC7425938
    • الرقم المعرف:
      10.1371/journal.pone.0234492
    • الرقم المعرف:
      32790760