Protective Effects of Some Grapevine Polyphenols against Naturally Occurring Neuronal Death.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c1995-

Cell Proliferation*; Cerebellum/*drug effects ; Neurons/*drug effects ; Neuroprotective Agents/*pharmacology ; Polyphenols/*pharmacology ; Vitis/*chemistry; Animals ; Animals, Newborn ; Cell Death ; Cells, Cultured ; Cerebellum/pathology ; Mice ; Neurons/pathology

The interest in the biological properties of grapevine polyphenols (PPs) in neuroprotection is continuously growing in the hope of finding translational applications. However, there are several concerns about the specificity of action of these molecules that appear to act non-specifically on the permeability of cellular membranes. Naturally occurring neuronal death (NOND) during cerebellar maturation is a well characterized postnatal event that is very useful to investigate the death and rescue of neurons. We here aimed to establish a baseline comparative study of the potential to counteract NOND of certain grapevine PPs of interest for the oenology. To do so, we tested ex vivo the neuroprotective activity of peonidin- and malvidin-3-O-glucosides, resveratrol, polydatin, quercetin-3-O-glucoside, (+)-taxifolin, and (+)-catechin. The addition of these molecules (50 μM) to organotypic cultures of mouse cerebellum explanted at postnatal day 7, when NOND reaches a physiological peak, resulted in statistically significant (two-tailed Mann-Whitney test-p < 0.001) reductions of the density of dead cells (propidium iodide ⁺ cells/mm ² ) except for malvidin-3-O-glucoside. The stilbenes were less effective in reducing cell death (to 51-60%) in comparison to flavanols, (+)-taxifolin and quercetin 3-O-glucoside (to 69-72%). Thus, molecules with a -OH group in ortho position (taxifolin, quercetin 3-O-glucoside, (+)-catechin, and peonidin 3-O-glucoside) have a higher capability to limit death of cerebellar neurons. As NOND is apoptotic, we speculate that PPs act by inhibiting executioner caspase 3.

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UniTO Local Funds Università degli Studi di Torino

Keywords: (+)-catechin; (+)-taxifolin; apoptosis; cerebellum; malvidin 3-O-glucoside; neuronal death; peonidin 3-O-glucoside; polydatin; quercetin 3-O-glucoside; resveratrol

0 (Neuroprotective Agents)
0 (Polyphenols)

Date Created: 20200708 Date Completed: 20210326 Latest Revision: 20240329

20240329

PMC7356852

10.3390/molecules25122925

32630488