SARS-CoV-2 surveillance for a non-human primate breeding research facility.

Publisher: Wiley Country of Publication: Denmark NLM ID: 0320626 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-0684 (Electronic) Linking ISSN: 00472565 NLM ISO Abbreviation: J Med Primatol Subsets: MEDLINE

Publication: Copenhagen : Wiley
Original Publication: Basel, New York, Karger.

Betacoronavirus/*isolation & purification ; Coronavirus Infections/*veterinary ; Macaca mulatta/*virology ; Monkey Diseases/*virology ; Pandemics/*veterinary ; Pneumonia, Viral/*veterinary; Animals ; Antibodies, Viral/blood ; Betacoronavirus/genetics ; Betacoronavirus/immunology ; COVID-19 ; Coronavirus Infections/virology ; Feces/virology ; Humans ; Pneumonia, Viral/virology ; RNA, Viral/isolation & purification ; SARS-CoV-2 ; Sentinel Surveillance/veterinary

Background: The emergence of SARS-CoV-2 and the ensuing COVID-19 pandemic prompted the need for a surveillance program to determine the viral status of the California National Primate Research Center non-human primate breeding colony, both for reasons of maintaining colony health and minimizing the risk of interference in COVID-19 and other research studies.
Methods: We collected biological samples from 10% of the rhesus macaque population for systematic testing to detect SARS-CoV-2 virus by RT-PCR and host antibody response by ELISA. Testing required the development and validation of new assays and an algorithm using in laboratory-developed and commercially available reagents and protocols.
Results and Conclusions: No SARS-CoV-2 RNA or antibody was detected in this study; therefore, we have proposed a modified testing algorithm for sentinel surveillance to monitor for any future transmissions. As additional reagents and controls become available, assay development and validation will continue, leading to the enhanced sensitivity, specificity, accuracy, and efficiency of testing.
(© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

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P51 OD011107-59 United States NH NIH HHS; P51 OD011107 United States OD NIH HHS; U42 OD010990 United States OD NIH HHS; R21 AI143454 United States AI NIAID NIH HHS; R21 AI143454-02S1 United States NH NIH HHS; 5U42OD010990 United States NH NIH HHS

Keywords: COVID-19; RNA PCR; antibody; management practices; testing algorithm

0 (Antibodies, Viral)
0 (RNA, Viral)

Date Created: 20200705 Date Completed: 20201127 Latest Revision: 20240922

20240922

PMC7361642

10.1111/jmp.12483

32621339