Significance of CD10 protein expression in the diagnostics of follicular lymphoma: A comparison of conventional immunohistochemistry with flow cytometry supported by the establishment of BCL2 and BCL6 rearrangements.

Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 101300213 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1751-553X (Electronic) Linking ISSN: 17515521 NLM ISO Abbreviation: Int J Lab Hematol Subsets: MEDLINE

Original Publication: Oxford : Blackwell Scientific Publications, c2007-

Flow Cytometry* ; Gene Expression Regulation, Neoplastic* ; Gene Rearrangement* ; Immunohistochemistry* ; Lymphoma, Follicular*/blood ; Lymphoma, Follicular*/diagnosis ; Lymphoma, Follicular*/genetics ; Lymphoma, Follicular*/pathology ; Neprilysin*/blood ; Neprilysin*/genetics ; Proto-Oncogene Proteins c-bcl-2*/blood ; Proto-Oncogene Proteins c-bcl-2*/genetics ; Proto-Oncogene Proteins c-bcl-6*/blood ; Proto-Oncogene Proteins c-bcl-6*/genetics; Female ; Humans ; Male ; Middle Aged

Introduction: Histopathological examination and immunohistochemistry (IHC) with a crucial role of CD10 expression remain a standard diagnostic tool in follicular lymphoma (FL). The results of IHC CD10 detection with different primary antibodies are not fully reproducible, but some reports show that flow cytometry (FCM) can be a reliable method of CD10 identification.
Methods: The aim of the study was to compare results of CD10 expression in FL by IHC and FCM including immunophenotypic features in the context of the BCL2 and BCL6 alterations.
Results: Out of 76 histopathologically diagnosed FL, a group of 25 cases had simultaneously FCM. Immunohistochemically 77.6% of cases were CD10-positive with comparable and reproducible results to FCM. Differences between the FCM expression of CD5/CD10/CD11c/CD25/CD43 and BCL2 overexpression (BCL2(+) ^higher ) correlated with the BCL2 and BCL6 rearrangements (R) status. Lack of CD10 expression corresponded with the absence of BCL2R and higher MUM1 expression by IHC results but had no clinical impact on the long-time outcomes.
Conclusions: Immunohistochemistry staining is a comparable method to FCM assessment in the evaluation of CD10 expression and diagnosis of FL. Fine-needle aspiration biopsy/FCM (FNAB/FCM) could be a useful tool for verifying FL diagnosis and CD10 detection. Despite its heterogeneity, FL has a characteristic immunophenotype. BCL2R and BCL6R FL cases differ mainly in levels of BCL2 and CD10 with CD43 co-expression; BCL2(+) ^higher by FCM correlates with BCL2R. Moreover, FNAB plays an important role in material provision for supportive karyotyping and BCL2R, BCL6R assessed by FISH.
(© 2020 John Wiley & Sons Ltd.)

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Keywords: CD10 expression; fine-needle aspiration biopsy; flow cytometry; follicular lymphoma; immunohistochemistry

0 (BCL2 protein, human)
0 (BCL6 protein, human)
0 (Proto-Oncogene Proteins c-bcl-2)
0 (Proto-Oncogene Proteins c-bcl-6)
EC 3.4.24.11 (Neprilysin)

Date Created: 20200505 Date Completed: 20210105 Latest Revision: 20210105

20250114

10.1111/ijlh.13222

32364682