Assessment of the microbial interplay during anaerobic co-digestion of wastewater sludge using common components analysis.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Archaea/*metabolism ; Bacteria/*metabolism ; Sewage/*microbiology; Anaerobiosis ; Archaea/isolation & purification ; Bacteria/isolation & purification ; Biodiversity ; Data Analysis ; Fisheries ; Microbial Interactions ; RNA, Bacterial ; RNA, Ribosomal, 16S ; Statistics as Topic

Anaerobic digestion (AD) is used to minimize solid waste while producing biogas by the action of microorganisms. To give an insight into the underlying microbial dynamics in anaerobic digesters, we investigated two different AD systems (wastewater sludge mixed with either fish or grass waste). The microbial activity was characterized by 16S RNA sequencing. 16S data is sparse and dispersed, and existent data analysis methods do not take into account this complexity nor the potential microbial interactions. In this line, we proposed a data pre-processing pipeline addressing these issues while not restricting only to the most abundant microorganisms. The data were analyzed by Common Components Analysis (CCA) to decipher the effect of substrate composition on the microorganisms. CCA results hinted the relationships between the microorganisms responding similarly to the AD physicochemical parameters. Thus, in overall, CCA allowed a better understanding of the inter-species interactions within microbial communities.
Competing Interests: The authors have declared that no competing interests exist.

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0 (RNA, Bacterial)
0 (RNA, Ribosomal, 16S)
0 (Sewage)

Date Created: 20200502 Date Completed: 20200803 Latest Revision: 20200803

20250114

PMC7194399

10.1371/journal.pone.0232324

32357180