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The ROS-associated programmed cell death causes the decline of pollen viability recovered from cryopreservation in Paeonia lactiflora.

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اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • المؤلفون: Ren R;Ren R; Li Z; Li Z; Jiang X; Jiang X; Liu Y; Liu Y
  • المصدر:
    Plant cell reports [Plant Cell Rep] 2020 Jul; Vol. 39 (7), pp. 941-952. Date of Electronic Publication: 2020 Apr 15.
  • نوع النشر :
    Journal Article
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-203X (Electronic) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Berlin ; New York : Springer, 1981-
    • الموضوع:
      Apoptosis*/drug effects ; Cryopreservation* ; Tissue Survival*/drug effects; Paeonia/*cytology ; Paeonia/*physiology ; Pollen/*cytology ; Pollen/*physiology ; Reactive Oxygen Species/*metabolism; Antioxidants/metabolism ; Ascorbic Acid/pharmacology ; Glutathione/pharmacology ; Humidity ; Oxidative Stress/drug effects ; Paeonia/drug effects ; Pollen/drug effects
    • نبذة مختصرة :
      Key Message: After cryopreservation, the occurrence of apoptosis-like programmed cell death events induced by the accumulation of ROS reduces pollen viability. Cryopreservation, as a biotechnological means for long-term preservation of pollen, has been applied to many species. However, after cryopreservation, the viability of pollen significantly decreases via a mechanism that is not completely clear. In this study, the pollen of Paeonia lactiflora 'Zi Feng Chao Yang', which exhibits significantly reduced viability after liquid nitrogen (LN 2 ) storage, was used to study the relationship among pollen viability, programmed cell death (PCD) and reactive oxygen species (ROS). The apoptosis rate was increased significantly in pollen with decreased viability after cryopreservation, and the changes in ROS generation and hydrogen peroxide (H 2 O 2 ) were consistent with the apoptosis rate. Correlation analysis results showed that the apoptosis rate is positively correlated with ROS generation and H 2 O 2 content. In addition, ascorbic acid (AsA), glutathione (GSH) and ascorbic acid reductase (APX) levels were significantly correlated with ROS and H 2 O 2 . After LN 2 preservation for 8 months, the exogenous antioxidants AsA and GSH at appropriate concentrations significantly decreased H 2 O 2 content, inhibited PCD indicator levels, and increased cryopreserved pollen viability. These observations suggest that PCD occurred in pollen during LN 2 preservation for 1-8 months and was induced by the accumulation of ROS in pollen after cryopreservation, thus explaining the main reasons for the reduction in pollen viability after cryopreservation in LN 2 .
    • Grant Information:
      31770741 National Natural Science Foundation of China; 31370693 National Natural Science Foundation of China
    • Contributed Indexing:
      Keywords: Antioxidant; Cryopreservation; Germplasm preservation; Pollen; Programmed cell death; Reactive oxygen species; Viability
    • الرقم المعرف:
      0 (Antioxidants)
      0 (Reactive Oxygen Species)
      GAN16C9B8O (Glutathione)
      PQ6CK8PD0R (Ascorbic Acid)
    • الموضوع:
      Date Created: 20200417 Date Completed: 20210122 Latest Revision: 20220415
    • الموضوع:
      20250114
    • الرقم المعرف:
      10.1007/s00299-020-02540-0
    • الرقم المعرف:
      32296871