A Preliminary Study of the Virome of the South American Free-Tailed Bats ( Tadarida brasiliensis ) and Identification of Two Novel Mammalian Viruses.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI

Metagenome* ; Metagenomics*/methods ; Virome*; Chiroptera/*virology; Animals ; Argentina ; Gene Order ; Genome, Viral ; High-Throughput Nucleotide Sequencing ; Open Reading Frames ; Papillomaviridae/classification ; Papillomaviridae/genetics ; Phylogeny ; Sequence Analysis, DNA ; Whole Genome Sequencing ; Workflow

Bats provide important ecosystem services as pollinators, seed dispersers, and/or insect controllers, but they have also been found harboring different viruses with zoonotic potential. Virome studies in bats distributed in Asia, Africa, Europe, and North America have increased dramatically over the past decade, whereas information on viruses infecting South American species is scarce. We explored the virome of Tadarida brasiliensis , an insectivorous New World bat species inhabiting a maternity colony in Rosario (Argentina), by a metagenomic approach. The analysis of five pooled oral/anal swab samples indicated the presence of 43 different taxonomic viral families infecting a wide range of hosts. By conventional nucleic acid detection techniques and/or bioinformatics approaches, the genomes of two novel viruses were completely covered clustering into the Papillomaviridae ( Tadarida brasiliensis papillomavirus type 1, TbraPV1) and Genomoviridae ( Tadarida brasiliensis gemykibivirus 1, TbGkyV1) families. TbraPV1 is the first papillomavirus type identified in this host and the prototype of a novel genus. TbGkyV1 is the first genomovirus reported in New World bats and constitutes a new species within the genus Gemykibivirus . Our findings extend the knowledge about oral/anal viromes of a South American bat species and contribute to understand the evolution and genetic diversity of the novel characterized viruses.

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Keywords: Genomoviridae; Papillomaviridae; Tadarida brasiliensis; TbGkyV1; TbraPV1; metagenomics

Date Created: 20200415 Date Completed: 20210222 Latest Revision: 20210222

20231215

PMC7232368

10.3390/v12040422

32283670