West Nile Virus Vaccine Design by T Cell Epitope Selection: In Silico Analysis of Conservation, Functional Cross-Reactivity with the Human Genome, and Population Coverage.

Publisher: Wiley Country of Publication: Egypt NLM ID: 101627166 Publication Model: eCollection Cited Medium: Internet ISSN: 2314-7156 (Electronic) Linking ISSN: 23147156 NLM ISO Abbreviation: J Immunol Res Subsets: MEDLINE

Publication: 2024- : [Hoboken, NJ] : Wiley
Original Publication: Cairo, Egypt : Hindawi Publishing Corporation, [2014]-

CD4-Positive T-Lymphocytes/*immunology ; CD8-Positive T-Lymphocytes/*immunology ; West Nile Fever/*immunology ; West Nile Virus Vaccines/*immunology ; West Nile virus/*physiology; Cells, Cultured ; Conserved Sequence ; Cross Reactions ; Demography ; Enzyme-Linked Immunospot Assay ; Epitope Mapping ; Epitopes/chemistry ; Epitopes/metabolism ; Genome ; HLA Antigens/metabolism ; Humans ; Protein Binding ; United Kingdom ; Viral Proteins/chemistry ; Viral Proteins/metabolism

West Nile Virus (WNV) causes a debilitating and life-threatening neurological disease in humans. Since its emergence in Africa 50 years ago, new strains of WNV and an expanding geographical distribution have increased public health concerns. There are no licensed therapeutics against WNV, limiting effective infection control. Vaccines represent the most efficacious and efficient medical intervention known. Epitope-based vaccines against WNV remain significantly underexploited. Here, we use a selection protocol to identify a set of conserved prevalidated immunogenic T cell epitopes comprising a putative WNV vaccine. Experimentally validated immunogenic WNV epitopes and WNV sequences were retrieved from the IEDB and West Nile Virus Variation Database. Clustering and multiple sequence alignment identified a smaller subset of representative sequences. Protein variability analysis identified evolutionarily conserved sequences, which were used to select a diverse set of immunogenic candidate T cell epitopes. Cross-reactivity and human leukocyte antigen-binding affinities were assessed to eliminate unsuitable epitope candidates. Population protection coverage (PPC) quantified individual epitopes and epitope combinations against the world population. 3 CD8+ T cell epitopes (ITYTDVLRY, TLARGFPFV, and SYHDRRWCF) and 1 CD4+ epitope (VTVNPFVSVATANAKVLI) were selected as a putative WNV vaccine, with an estimated PPC of 97.14%.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2020 Frances M. Waller et al.)

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0 (Epitopes)
0 (HLA Antigens)
0 (Viral Proteins)
0 (West Nile Virus Vaccines)

Date Created: 20200408 Date Completed: 20201211 Latest Revision: 20201214

20250114

PMC7106935

10.1155/2020/7235742

32258174