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miR-1285-3p Controls Colorectal Cancer Proliferation and Escape from Apoptosis through DAPK2.

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اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Basel, Switzerland : MDPI, [2000-
    • الموضوع:
      Apoptosis/*physiology ; Cell Proliferation/*physiology ; Colorectal Neoplasms/*genetics ; Colorectal Neoplasms/*metabolism ; Death-Associated Protein Kinases/*metabolism ; MicroRNAs/*genetics ; MicroRNAs/*metabolism; Antineoplastic Agents/pharmacology ; Apoptosis/drug effects ; Cell Cycle Checkpoints ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Colonic Neoplasms/genetics ; Colonic Neoplasms/metabolism ; Death-Associated Protein Kinases/genetics ; Gene Expression Regulation, Neoplastic/drug effects ; Humans ; Neoplastic Stem Cells ; Oligonucleotides
    • نبذة مختصرة :
      MicroRNAs are tiny but powerful regulators of gene expression at the post-transcriptional level. Aberrant expression of oncogenic and tumor-suppressor microRNAs has been recognized as a common feature of human cancers. Colorectal cancer represents a major clinical challenge in the developed world and the design of innovative therapeutic approaches relies on the identification of novel biological targets. Here, we perform a functional screening in colorectal cancer cells using a library of locked nucleic acid (LNA)-modified anti-miRs in order to unveil putative oncogenic microRNAs whose inhibition yields a cytotoxic effect. We identify miR-1285-3p and further explore the effect of its targeting in both commercial cell lines and primary colorectal cancer stem cells, finding induction of cell cycle arrest and apoptosis. We show that DAPK2, a known tumor-suppressor, is a novel miR-1285 target and mediates both the anti-proliferative and the pro-apoptotic effects of miR-1285 depletion. Altogether, our findings uncover a novel oncogenic microRNA in colorectal cancer and lay the foundation for further studies aiming at the development of possible therapeutic strategies based on miR-1285 targeting.
      Competing Interests: Supplementary Materials: The following are available online at www.mdpi.com/1422-0067/21/7/2423/s1.
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    • Grant Information:
      Investigator Grant 13431 Associazione Italiana per la Ricerca sul Cancro
    • Contributed Indexing:
      Keywords: DAPK2; LNAs; apoptosis; cancer stem cells; cell cycle; colorectal cancer; microRNAs
    • الرقم المعرف:
      0 (Antineoplastic Agents)
      0 (MIRN1285 microRNA, human)
      0 (MicroRNAs)
      0 (Oligonucleotides)
      0 (locked nucleic acid)
      EC 2.7.11.1 (DAPK2 protein, human)
      EC 2.7.11.1 (Death-Associated Protein Kinases)
    • الموضوع:
      Date Created: 20200405 Date Completed: 20210107 Latest Revision: 20210107
    • الموضوع:
      20240628
    • الرقم المعرف:
      PMC7177834
    • الرقم المعرف:
      10.3390/ijms21072423
    • الرقم المعرف:
      32244500