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Octominin Inhibits LPS-Induced Chemokine and Pro-inflammatory Cytokine Secretion from RAW 264.7 Macrophages via Blocking TLRs/NF-κB Signal Transduction.
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- معلومة اضافية
- المصدر:
Publisher: MDPI Country of Publication: Switzerland NLM ID: 101596414 Publication Model: Electronic Cited Medium: Internet ISSN: 2218-273X (Electronic) Linking ISSN: 2218273X NLM ISO Abbreviation: Biomolecules Subsets: MEDLINE
- بيانات النشر:
Original Publication: Basel, Switzerland : MDPI, 2011-
- الموضوع:
- نبذة مختصرة :
Inflammation is a well-organized innate immune response that plays an important role during the pathogen attacks and mechanical injuries. The Toll-like receptors (TLR)/nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) is a major signal transduction pathway observed in RAW 264.7 macrophages during the inflammatory responses. Here, we investigated the anti-inflammatory effects of Octominin; a bio-active peptide developed from Octopus minor in RAW 264.7 macrophages in vitro. Octominin was found to inhibit lipopolysaccharides (LPS)-stimulated transcriptional activation of NF-κB in RAW 264.7 cells and dose-dependently decreased the mRNA expression levels of TLR4. Specifically, in silico docking results demonstrated that Octominin has a potential to inhibit TLR4 mediated inflammatory responses via blocking formation of TLR4/MD-2/LPS complex. We also demonstrated that Octominin could significantly inhibit LPS-induced secretion of pro-inflammatory cytokine (interleukin-β; IL-1β, IL-6, and tumor necrosis factor-α) and chemokines (CCL3, CCL4, CCL5, and CXCL10) from RAW 264.7 cells. Additionally, Octominin repressed the LPS-induced pro-inflammatory mediators including nitric oxide (NO), prostaglandin E2, inducible NO synthase, and cyclooxygenase 2 in macrophages. These results suggest that Octominin is a potential inhibitor of TLRs/NF-κB signal transduction pathway and is a potential candidate for the treatment of inflammatory diseases.
Competing Interests: The authors declare no conflict of interest.
- References:
Trends Mol Med. 2010 Mar;16(3):133-44. (PMID: 20163989)
Cell. 2007 Sep 7;130(5):906-17. (PMID: 17803912)
J Immunol Res. 2014;2014:149185. (PMID: 24901008)
Nature. 2009 Apr 30;458(7242):1191-5. (PMID: 19252480)
Vet Immunol Immunopathol. 2003 Jan 10;91(1):1-12. (PMID: 12507844)
Int J Cancer. 2007 Dec 1;121(11):2357-63. (PMID: 17893865)
J Dairy Sci. 2015 Oct;98(10):6631-50. (PMID: 26210279)
J Ethnopharmacol. 2017 Mar 6;199:30-38. (PMID: 28119099)
Cytokine. 2018 Jul;107:18-25. (PMID: 29398279)
RSC Adv. 2019 Mar 18;9(16):8912-8925. (PMID: 35517648)
Microbes Infect. 2002 Jul;4(8):837-51. (PMID: 12270731)
Int Immunopharmacol. 2019 Mar;68:156-163. (PMID: 30639961)
Semin Liver Dis. 2010 Aug;30(3):215-25. (PMID: 20665374)
Nat Rev Rheumatol. 2016 Jun;12(6):344-57. (PMID: 27170508)
Gigascience. 2018 Nov 1;7(11):. (PMID: 30256935)
Nat Rev Neurol. 2011 Jan;7(1):31-40. (PMID: 21135885)
Nature. 2015 Jan 15;517(7534):311-20. (PMID: 25592536)
J Orthop Res. 2017 Apr;35(4):735-739. (PMID: 27808445)
PLoS One. 2015 May 27;10(5):e0126871. (PMID: 26017270)
Sci Rep. 2019 Jan 29;9(1):919. (PMID: 30696900)
Mar Drugs. 2020 Jan 15;18(1):. (PMID: 31952292)
Annu Rev Biochem. 2002;71:635-700. (PMID: 12045108)
Mediators Inflamm. 2010;2010:393946. (PMID: 20652007)
Int J Biol Macromol. 2018 Feb;107(Pt A):803-807. (PMID: 28962845)
Clin Microbiol Rev. 2003 Oct;16(4):637-46. (PMID: 14557290)
FASEB J. 1994 Feb;8(2):217-25. (PMID: 8119492)
J Interferon Cytokine Res. 2009 Jun;29(6):313-26. (PMID: 19441883)
Nat Rev Microbiol. 2005 Jan;3(1):36-46. (PMID: 15608698)
Int J Inflam. 2016;2016:1532832. (PMID: 27795867)
Nat Rev Rheumatol. 2014 Jan;10(1):44-56. (PMID: 24166242)
Mar Drugs. 2015 Jun 29;13(7):4006-43. (PMID: 26132844)
Microbes Infect. 2004 Dec;6(15):1361-7. (PMID: 15596121)
Peptides. 2009 May;30(5):832-8. (PMID: 19428758)
Front Immunol. 2019 Jun 14;10:1365. (PMID: 31258535)
Science. 2007 Jun 15;316(5831):1632-4. (PMID: 17569869)
Nature. 1997 Jul 24;388(6640):394-7. (PMID: 9237759)
Molecules. 2018 Jan 31;23(2):. (PMID: 29385101)
Life Sci. 2007 Oct 27;81(19-20):1431-5. (PMID: 17961604)
Nat Rev Rheumatol. 2011 Jan;7(1):33-42. (PMID: 21119608)
Tissue Eng Part A. 2018 Nov;24(21-22):1641-1651. (PMID: 29766758)
Int J Chron Obstruct Pulmon Dis. 2016 Jun 23;11:1391-401. (PMID: 27382275)
Mediators Inflamm. 2015;2015:167572. (PMID: 26612970)
Food Funct. 2020 Jan 29;11(1):552-560. (PMID: 31850468)
Exp Cell Res. 2011 Mar 10;317(5):664-73. (PMID: 21134366)
- Grant Information:
MABIK2020M00600 and 2019R1A2C1087028 International This work was supported by the Research Program of the National Marine Biodiversity Institute of Korea (MABIK2020M00600) funded by the Ministry of Oceans and Fisheries and by the National Research Foundation of Korea (NRF) grant funded by the Korea govern
- Contributed Indexing:
Keywords: NF-κB; Octopus minor; RAW 264.7; chemokines; inflammation; peptide
- الرقم المعرف:
0 (Anti-Inflammatory Agents, Non-Steroidal)
0 (Antimicrobial Cationic Peptides)
0 (Chemokines)
0 (Cytokines)
0 (Inflammation Mediators)
0 (Lipopolysaccharides)
0 (Ly96 protein, mouse)
0 (Lymphocyte Antigen 96)
0 (NF-kappa B)
0 (Peptide Fragments)
0 (Peptides)
0 (Tlr4 protein, mouse)
0 (Toll-Like Receptor 4)
0 (octominin)
31C4KY9ESH (Nitric Oxide)
EC 1.14.13.39 (Nitric Oxide Synthase Type II)
EC 1.14.13.39 (Nos2 protein, mouse)
K7Q1JQR04M (Dinoprostone)
- الموضوع:
Date Created: 20200402 Date Completed: 20210415 Latest Revision: 20240328
- الموضوع:
20240329
- الرقم المعرف:
PMC7226457
- الرقم المعرف:
10.3390/biom10040511
- الرقم المعرف:
32230927
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