Proteomic mapping of Drosophila transgenic elav.L-GAL4/+ brain as a tool to illuminate neuropathology mechanisms.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Proteolysis*; Brain/*metabolism ; Drosophila/*genetics ; Drosophila Proteins/*metabolism ; ELAV Proteins/*genetics ; Nervous System Diseases/*etiology ; Nervous System Diseases/*genetics ; Proteasome Endopeptidase Complex/*genetics ; Proteomics/*methods ; Transcription Factors/*genetics ; Ubiquitination/*genetics; Animals ; Animals, Genetically Modified ; Drosophila/physiology ; Drosophila Proteins/genetics ; Female ; Humans ; Locomotion/genetics ; Longevity/genetics ; Male ; RNA Interference ; Ubiquitin/metabolism

Drosophila brain has emerged as a powerful model system for the investigation of genes being related to neurological pathologies. To map the proteomic landscape of fly brain, in a high-resolution scale, we herein employed a nano liquid chromatography-tandem mass spectrometry technology, and high-content catalogues of 7,663 unique peptides and 2,335 single proteins were generated. Protein-data processing, through UniProt, DAVID, KEGG and PANTHER bioinformatics subroutines, led to fly brain-protein classification, according to sub-cellular topology, molecular function, implication in signaling and contribution to neuronal diseases. Given the importance of Ubiquitin Proteasome System (UPS) in neuropathologies and by using the almost completely reassembled UPS, we genetically targeted genes encoding components of the ubiquitination-dependent protein-degradation machinery. This analysis showed that driving RNAi toward proteasome components and regulators, using the GAL4-elav.L driver, resulted in changes to longevity and climbing-activity patterns during aging. Our proteomic map is expected to advance the existing knowledge regarding brain biology in animal species of major translational-research value and economical interest.

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P40 OD018537 United States OD NIH HHS

0 (Drosophila Proteins)
0 (ELAV Proteins)
0 (ELAV protein, Drosophila)
0 (GAL4 protein, Drosophila)
0 (Transcription Factors)
0 (Ubiquitin)
EC 3.4.25.1 (Proteasome Endopeptidase Complex)

Date Created: 20200328 Date Completed: 20201216 Latest Revision: 20210325

20231215

PMC7096425

10.1038/s41598-020-62510-0

32214222