Improved detection of esp, hyl, asa1, gelE, cylA virulence genes among clinical isolates of Enterococci.

Publisher: Biomed Central Country of Publication: England NLM ID: 101462768 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-0500 (Electronic) Linking ISSN: 17560500 NLM ISO Abbreviation: BMC Res Notes Subsets: MEDLINE

Original Publication: London : Biomed Central, 2008.

Bacterial Proteins*/isolation & purification ; Genes, Bacterial*; Enterococcus faecalis/*pathogenicity ; Enterococcus faecium/*pathogenicity ; Virulence/*genetics; Enterococcus faecalis/isolation & purification ; Enterococcus faecium/isolation & purification ; Humans ; India ; Membrane Proteins/isolation & purification ; Multiplex Polymerase Chain Reaction

Objective: Virulence factors (VFs) among the clinical strains of enterococci play a vital role in pathogenesis. This study was aimed to screen for cylA, asa1, gelE, esp and hyl among Enterococcus faecalis (n = 89) and E. faecium (n = 51) by multiplex PCR. The previously reported multiplex PCR was modified to 2 duplex (asa1 and gelE, cylA and esp) PCRs and 1 simplex (hyl) PCR. The idea of the modification of the multiplex PCR proposed here emerged in the course of the research study when majority of the isolates which phenotypically exhibited virulence traits were found to be negative for the respective gene.
Results: cylA, gelE and asa1 were significantly predominant in E. faecalis (59.55%, 85.39%, 86.51%) than E. faecium (1.96%, 60.78%, 9.80%) (p < 0.0001, p = 0.001967, p < 0.0001). hyl was detected in E. faecium (5.9%) only. The number of VFs detected in each isolate was recorded as the VF score. E. faecalis isolates had a VF score pattern of score 4 (34.83%), score 3 (26.96%), score 2 (28.08%) and score 1 (8.98%) while E. faecium had score 4 (1.96%), score 3 (7.84%), score 2 (25.49%) and score 1 (41.18%). This modification of the PCR protocol could resolve the problem of decreased detection of virulence determinants in enterococci.

J Clin Microbiol. 1989 Apr;27(4):731-4. (PMID: 2656745)
J Glob Infect Dis. 2016 Jan-Mar;8(1):16-24. (PMID: 27013840)
Int J Food Microbiol. 2009 Jun 1;132(1):24-32. (PMID: 19375810)
Int J Antimicrob Agents. 2008 Nov;32(5):374-7. (PMID: 18715765)
Indian J Med Microbiol. 2012 Apr-Jun;30(2):175-81. (PMID: 22664433)
BMC Res Notes. 2018 Jan 2;11(1):1. (PMID: 29291749)
J Bacteriol. 1990 Jan;172(1):155-63. (PMID: 2152897)
Infect Immun. 1991 Jan;59(1):415-20. (PMID: 1846126)
J Infect Dis. 2003 Feb 1;187(3):508-12. (PMID: 12552437)
Interdiscip Perspect Infect Dis. 2019 Mar 3;2019:7854968. (PMID: 30941169)
Biomed Res Int. 2019 May 9;2019:2815279. (PMID: 31211134)
J Clin Microbiol. 1987 Aug;25(8):1524-8. (PMID: 3114322)
Infect Immun. 1992 Jan;60(1):25-30. (PMID: 1729187)
Indian J Pathol Microbiol. 2016 Jan-Mar;59(1):50-5. (PMID: 26960635)
Lancet. 2001 Mar 17;357(9259):853-5. (PMID: 11265956)
J Food Prot. 2011 Mar;74(3):465-9. (PMID: 21375886)
J Vet Sci. 2019 May;20(3):e26. (PMID: 31161744)
BMC Infect Dis. 2019 Aug 27;19(1):744. (PMID: 31455296)
Infect Chemother. 2017 Mar;49(1):51-56. (PMID: 28332345)
J Pathog. 2015;2015:692612. (PMID: 26366302)
J Infect Dis. 1995 May;171(5):1223-9. (PMID: 7751697)
Onderstepoort J Vet Res. 2018 Jun 27;85(1):e1-e8. (PMID: 30035595)
Infect Immun. 1992 Jun;60(6):2445-52. (PMID: 1587612)
Infect Immun. 1989 Jun;57(6):1834-8. (PMID: 2498209)
Antimicrob Agents Chemother. 1991 Aug;35(8):1626-34. (PMID: 1929336)
Infect Immun. 2001 Jul;69(7):4366-72. (PMID: 11401975)
J Clin Pathol. 1989 Aug;42(8):872-4. (PMID: 2475530)
Acta Pathol Microbiol Scand B. 1979 Dec;87(6):353-62. (PMID: 120105)
J Clin Microbiol. 2004 Oct;42(10):4473-9. (PMID: 15472296)
J Bacteriol. 2007 Dec;189(24):8835-43. (PMID: 17921295)
Microbiol Mol Biol Rev. 1997 Jun;61(2):136-69. (PMID: 9184008)
Appl Environ Microbiol. 2001 Apr;67(4):1628-35. (PMID: 11282615)
Infect Immun. 1990 Dec;58(12):3914-23. (PMID: 2123826)
J Infect Dis. 1994 Dec;170(6):1549-56. (PMID: 7995995)
Mol Microbiol. 1990 Jun;4(6):895-904. (PMID: 2120541)
Antimicrob Agents Chemother. 1993 Nov;37(11):2474-7. (PMID: 8285637)
Microb Drug Resist. 2019 Jul/Aug;25(6):880-889. (PMID: 30811265)
FEMS Microbiol Lett. 2000 Feb 15;183(2):201-7. (PMID: 10675584)
J Vet Med Sci. 2016 Dec 1;78(11):1663-1668. (PMID: 27476730)
Braz J Infect Dis. 2016 Mar-Apr;20(2):127-33. (PMID: 26849965)
Cell Mol Life Sci. 2003 Dec;60(12):2622-36. (PMID: 14685687)
Indian J Med Res. 2013 May;137(5):981-5. (PMID: 23760387)
Infect Immun. 1999 Jan;67(1):193-200. (PMID: 9864215)
J Pathog. 2016;2016:3437214. (PMID: 27144029)
Appl Environ Microbiol. 2001 Oct;67(10):4538-45. (PMID: 11571153)
J Dairy Sci. 2019 Jan;102(1):140-144. (PMID: 30415850)
Int J Food Microbiol. 2011 Dec 2;151(2):125-40. (PMID: 21962867)
Sci Rep. 2019 Aug 1;9(1):11204. (PMID: 31371744)
Front Microbiol. 2017 Nov 24;8:2338. (PMID: 29225595)
Res Microbiol. 2011 Feb-Mar;162(2):151-8. (PMID: 21111042)
Braz J Microbiol. 2014 Apr 18;45(1):327-32. (PMID: 24948952)

Keywords: E. faecalis; E. faecium; Modified PCR reactions; Virulence

0 (Bacterial Proteins)
0 (Membrane Proteins)
0 (aggregation substance, Enterococcus faecalis)
0 (enterococcal surface protein, esp)

Date Created: 20200322 Date Completed: 20201208 Latest Revision: 20201214

20250114

PMC7085142

10.1186/s13104-020-05018-0

32197635