Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Genetic variation in Austrostrongylus thylogale Johnston & Mawson, 1940 (Nematoda: Trichostrongylida) from the tammar wallaby, Notamacropus eugenii (Gray), and the quokka, Setonix brachyurus (Quoy & Gaimard) (Marsupialia: Macropodidae) in Australia.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : BioMed Central
    • الموضوع:
      Genetic Variation*; Macropodidae/*parasitology ; Nematoda/*classification ; Nematoda/*genetics; Animals ; Australia ; DNA, Helminth/genetics ; DNA, Ribosomal/genetics ; Host Specificity ; Macropodidae/classification ; Phylogeny ; Sequence Alignment ; Trichostrongyloidea
    • نبذة مختصرة :
      Background: Australian marsupials harbour a diverse array of helminth parasites. Despite current attempts to assess the extent of this diversity in macropodid hosts, it has been suggested that unique parasite fauna of Australian wildlife is difficult to document comprehensively due to the common occurrence of cryptic species. This paper assessed genetic variation within Austrostrongylus thylogale Johnston & Mawson, 1940 from the tammar wallaby, Notamacropus eugenii (Gray), and the quokka, Setonix brachyurus (Quoy & Gaimard), from different localities using the molecular characterisation of the internal transcribed spacers (ITS) within the nuclear ribosomal DNA.
      Methods: Thirty-seven specimens of A. thylogale collected from N. eugenii (from Parndana, Kangaroo Island, South Australia, and Perup, Western Australia) and S. brachyurus (from Wellington Dam, Western Australia) were characterised using a molecular-phylogenetic approach utilising the first (ITS1) and second (ITS2) internal transcribed spacers.
      Results: Genetic variation was detected in both ITS1 and ITS2 between specimens of A. thylogale from N. eugenii and S. brachyurus; however, no variation was detected between specimens collected from N. eugenii from Parndana, South Australia, and Perup, Western Australia. Furthermore, the phylogenetic analyses of ITS sequences showed two clades of A. thylogale originating from two hosts, N. eugenii and S. brachyurus, suggesting the presence of cryptic species.
      Conclusions: This study provides evidence of genetic variation within A. thylogale based on collections from two different host species. Morphological studies are required to fully confirm the presence of a new species or cryptic species. Further molecular studies using a larger number of specimens are warranted to explore the genetic variation between A. thylogale from different geographical localities.
    • References:
      Mol Biol Evol. 2018 Jun 1;35(6):1547-1549. (PMID: 29722887)
      Electrophoresis. 2012 Dec;33(23):3544-54. (PMID: 23161349)
      Nat Methods. 2012 Jul 30;9(8):772. (PMID: 22847109)
      Parasitol Res. 2009 Jan;104(2):229-36. (PMID: 18795332)
      Parasitology. 2017 Nov;144(13):1828-1840. (PMID: 28697818)
      Int J Parasitol. 2014 Oct 15;44(12):859-64. (PMID: 24992656)
      Anim Health Res Rev. 2004 Dec;5(2):173-87. (PMID: 15984323)
      Nucleic Acids Res. 1994 Oct 11;22(20):4354-5. (PMID: 7937169)
      Biotechnol Adv. 2008 Jul-Aug;26(4):325-34. (PMID: 18502076)
      Nucleic Acids Res. 2004 Mar 19;32(5):1792-7. (PMID: 15034147)
      Trends Parasitol. 2015 Apr;31(4):142-8. (PMID: 25435249)
      Syst Parasitol. 2002 Feb;51(2):135-47. (PMID: 11912339)
      Trends Ecol Evol. 2007 Mar;22(3):148-55. (PMID: 17129636)
      Zootaxa. 2016 Jun 15;4123(1):1-198. (PMID: 27395568)
      Int J Parasitol. 1995 Apr;25(4):483-8. (PMID: 7635624)
      Int J Parasitol. 1997 May;27(5):483-93. (PMID: 9193942)
      Int J Parasitol. 2001 Jul;31(9):976-96. (PMID: 11406145)
      Parasitol Int. 2011 Dec;60(4):381-7. (PMID: 21704186)
      Int J Parasitol. 1993 Feb;23(1):21-33. (PMID: 8468133)
      Int J Parasitol. 1997 Dec;27(12):1481-94. (PMID: 9467733)
      Mol Cell Probes. 1995 Oct;9(5):315-20. (PMID: 8569771)
      Bioinformatics. 2003 Aug 12;19(12):1572-4. (PMID: 12912839)
      Int J Parasitol. 1995 May;25(5):647-51. (PMID: 7635644)
    • Grant Information:
      RF217-06 Australian Biological Resources Study; CBG18-07 Australian Biological Resources Study
    • Contributed Indexing:
      Keywords: Austrostrongylus thylogale; Cryptic species; Internal transcribed spacers; Quokka; Tammar wallaby
    • الرقم المعرف:
      0 (DNA, Helminth)
      0 (DNA, Ribosomal)
    • الموضوع:
      Date Created: 20200316 Date Completed: 20201019 Latest Revision: 20201019
    • الموضوع:
      20221213
    • الرقم المعرف:
      PMC7071675
    • الرقم المعرف:
      10.1186/s13071-020-4007-5
    • الرقم المعرف:
      32171333