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Detection of melon necrotic spot virus by one-step reverse transcription loop-mediated isothermal amplification assay.

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  • معلومة اضافية
    • المصدر:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: San Francisco, CA : Public Library of Science
    • الموضوع:
      Nucleic Acid Amplification Techniques* ; Reverse Transcription*; Tombusviridae/*genetics ; Tombusviridae/*isolation & purification; Time Factors
    • نبذة مختصرة :
      Melon necrotic spot virus (MNSV) can cause significant economic losses due to decreased quality in cucurbit crops. The current study is the first to use reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of MNSV. A set of four LAMP primers was designed based on the coat protein gene sequence of MNSV, and a RT-LAMP reaction was successfully performed for 1 h at 62°C. The results of RT-LAMP showed high specificity for MNSV and no cross-reaction with other viruses. Compared to traditional reverse transcription-PCR (RT-PCR), the RT-LAMP assay was 103-fold more sensitive in detecting MNSV. Due to its sensitivity, speed and visual assessment, RT-LAMP is appropriate for detecting MNSV in the laboratory.
      Competing Interests: The authors have declared that no competing interests exist.
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    • الموضوع:
      Melon necrotic spot virus
    • الموضوع:
      Date Created: 20200306 Date Completed: 20200622 Latest Revision: 20200622
    • الموضوع:
      20240829
    • الرقم المعرف:
      PMC7058275
    • الرقم المعرف:
      10.1371/journal.pone.0230023
    • الرقم المعرف:
      32134962