Hyaluronic acid-coated nanostructured lipid carriers for loading multiple traditional Chinese medicine components for liver cancer treatment.

Publisher: Faculty of Pharmacy, University of Karachi Country of Publication: Pakistan NLM ID: 9426356 Publication Model: Print Cited Medium: Internet ISSN: 1011-601X (Print) Linking ISSN: 1011601X NLM ISO Abbreviation: Pak J Pharm Sci Subsets: MEDLINE

Original Publication: Karachi : Faculty of Pharmacy, University of Karachi, [1988-

Drug Carriers/*chemistry ; Ginsenosides/*chemistry ; Hyaluronic Acid/*chemistry ; Lipids/*chemistry ; Nanostructures/*chemistry ; Oleanolic Acid/*chemistry ; Triterpenes/*chemistry; Animals ; Antineoplastic Combined Chemotherapy Protocols/chemistry ; Antineoplastic Combined Chemotherapy Protocols/pharmacokinetics ; Antineoplastic Combined Chemotherapy Protocols/pharmacology ; Cell Line, Tumor ; Drug Liberation ; Female ; Ginsenosides/pharmacokinetics ; Ginsenosides/pharmacology ; Mice ; Neoplasms/metabolism ; Oleanolic Acid/pharmacokinetics ; Oleanolic Acid/pharmacology ; Particle Size ; Static Electricity ; Tissue Distribution ; Triterpenes/pharmacokinetics ; Triterpenes/pharmacology ; Ursolic Acid

This study aimed to develop hyaluronic acid (HA)-coated nanostructured lipid carriers (NLC) loaded simultaneously with oleanolic acid (OA), ursolic acid (UA) and Ginsenoside Rg3 (Rg3), prepared by electrostatic attraction for delivering OA, UA and Rg3 (OUR), termed HA-OUR-NLC, to tumors over expressing cluster determinant 44(CD44). The dialysis method was used to assess the in vitro release of OUR. Parameters such as pharmacokinetics, biodistribution, fluorescence in vivo endo-microscopy (FIVE), optical in vivo imaging (OIVI) data, and in vivo antitumor effects were evaluated. The results showed a total drug loading rate of 8.76±0.95% for the optimized HA-OUR-NLC; total encapsulation efficiency was 45.67±1.14%; particle size was 165.15±3.84%; polydispersity index was 0.227±0.01; zeta potential was -22.87±0.97 mV. Drug release followed the Higuchi kinetics. Pharmacokinetics and tissue distribution, as well as antitumor effects were evaluated in nude mice in vivo. HA-OUR-NLC were better tolerated, with increased antitumor activity compared with 5-Fu. In in vivo optical imaging, we use 1,1'-dioctadecyl-3,3,3',3'-tetramethy(DiR) as a fluorescent dye to label the NLC. The DiR-OUR-NLC group showed bright systemic signals, while the tumor site was weak. The present findings indicated that HA-OUR-NLC accumulated in the tumor site, prolonging OUR duration in the circulation and enhancing tumoral concentrations. Therefore, NLC prepared by electrostatic attraction constitute a good system for delivering OUR to tumors.

0 (Drug Carriers)
0 (Ginsenosides)
0 (Lipids)
0 (Triterpenes)
227D367Y57 (ginsenoside Rg3)
6SMK8R7TGJ (Oleanolic Acid)
9004-61-9 (Hyaluronic Acid)

Date Created: 20200304 Date Completed: 20201221 Latest Revision: 20231213

20231215

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