Short-Term Responses to Fatty Acids on Lipid Metabolism and Adipogenesis in Rainbow Trout ( Oncorhynchus mykiss ).

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Adipocytes/*drug effects ; Adipogenesis/*drug effects ; Docosahexaenoic Acids/*metabolism ; Eicosapentaenoic Acid/*administration & dosage ; Lipid Metabolism/*drug effects ; Oncorhynchus mykiss/*metabolism ; alpha-Linolenic Acid/*administration & dosage; Adipocytes/metabolism ; Adipose Tissue/metabolism ; Animals ; CD36 Antigens/genetics ; CD36 Antigens/metabolism ; Cells, Cultured ; Diet ; Docosahexaenoic Acids/pharmacology ; Eicosapentaenoic Acid/metabolism ; Eicosapentaenoic Acid/pharmacology ; Fatty Acid Synthases/genetics ; Fatty Acid Synthases/metabolism ; Fatty Acid Transport Proteins/genetics ; Fatty Acid Transport Proteins/metabolism ; Fatty Acids/metabolism ; Fatty Acids/pharmacology ; Liver/metabolism ; Peroxisome Proliferator-Activated Receptors/genetics ; Peroxisome Proliferator-Activated Receptors/metabolism ; Plasma/drug effects ; Plasma/metabolism ; alpha-Linolenic Acid/metabolism ; alpha-Linolenic Acid/pharmacology

Fish are rich in n-3 long-chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids. Due to the increasing use of vegetable oils (VO), their proportion in diets has lowered, affecting lipid metabolism and fillet composition. Rainbow trout cultured preadipocytes were treated with representative FA found in fish oils (EPA and DHA) or VO (linoleic, LA and alpha-linolenic, ALA acids), while EPA and LA were also orally administered, to evaluate their effects on adipogenesis and lipid metabolism. In vitro, all FA increased lipid internalization, with ALA producing the highest effect, together with upregulating the FA transporter fatp1 . In vivo, EPA or LA increased peroxisome proliferator-activated receptors ppara and pparb transcripts abundance in adipose tissue, suggesting elevated β-oxidation, contrary to the results obtained in liver. Furthermore, the increased expression of FA synthase ( fas ) and the FA translocase/cluster of differentiation ( cd36) in adipose tissue indicated an enhanced uptake of lipids and lipogenesis de novo, whereas stable or low hepatic expression of genes involved in lipid transport and turnover was found. Thus, fish showed a similar tissue metabolic response to the short-term availability of EPA or LA in vivo, while in vitro VO-derived FA demonstrated greater potential inducing fat accumulation.

PLoS One. 2013;8(3):e59507. (PMID: 23533628)
Crit Rev Biochem Mol Biol. 2005 Jul-Aug;40(4):229-42. (PMID: 16126487)
Int J Mol Sci. 2018 Jul 21;19(7):. (PMID: 30037087)
Amino Acids. 2010 Aug;39(3):801-10. (PMID: 20213441)
J Mol Neurosci. 2001 Apr-Jun;16(2-3):117-21; discussion 151-7. (PMID: 11478366)
Lipids Health Dis. 2014 Jan 03;13:3. (PMID: 24387137)
Br J Nutr. 2008 Nov;100(5):992-1003. (PMID: 18377678)
Lipids. 2004 Nov;39(11):1077-83. (PMID: 15726822)
Comp Biochem Physiol A Mol Integr Physiol. 2002 Aug;132(4):913-9. (PMID: 12095871)
J Endocrinol. 2015 Mar;224(3):R97-106. (PMID: 25486966)
Comp Biochem Physiol A Mol Integr Physiol. 2013 Jan;164(1):241-8. (PMID: 23010243)
Biochem J. 1997 Jun 15;324 ( Pt 3):911-5. (PMID: 9210416)
Cell Death Differ. 2016 Jul;23(7):1128-39. (PMID: 26868907)
Domest Anim Endocrinol. 2013 Jul;45(1):1-10. (PMID: 23535263)
J Nutr. 2004 Sep;134(9):2444S-2449S. (PMID: 15333740)
Lipids. 2000 Jun;35(6):653-64. (PMID: 10901428)
PLoS One. 2019 Apr 24;14(4):e0215926. (PMID: 31017945)
Comp Biochem Physiol A Mol Integr Physiol. 2005 May;141(1):108-15. (PMID: 15922639)
Biochim Biophys Acta. 2005 May 30;1740(2):266-86. (PMID: 15949694)
Lipids. 2003 Mar;38(3):289-96. (PMID: 12784870)
BMC Genomics. 2017 May 4;18(1):347. (PMID: 28472935)
Am J Physiol Regul Integr Comp Physiol. 2012 Jun;302(11):R1340-50. (PMID: 22496361)
Nutr J. 2014 Feb 14;13:17. (PMID: 24524207)
J Physiol Biochem. 2016 Sep;72(3):485-94. (PMID: 27312217)
Sci Rep. 2016 Feb 22;6:21892. (PMID: 26899924)
Philos Trans R Soc Lond B Biol Sci. 2010 Sep 27;365(1554):2897-912. (PMID: 20713392)
J Clin Med. 2015 Dec 31;5(1):. (PMID: 26729182)
Biochim Biophys Acta. 2008 Jun-Jul;1781(6-7):326-35. (PMID: 18503782)
J Appl Physiol (1985). 1999 Jul;87(1):227-32. (PMID: 10409579)
Nutrients. 2015 Nov 13;7(11):9453-74. (PMID: 26580649)
Br J Nutr. 2000 Aug;84(2):175-84. (PMID: 11029968)
Diabetes. 2001 Feb;50(2):367-75. (PMID: 11272149)
Comp Biochem Physiol B Biochem Mol Biol. 2006 Jul;144(3):326-33. (PMID: 16716627)
PLoS One. 2016 Jun 02;11(6):e0156765. (PMID: 27253325)
J Exp Biol. 2012 Apr 15;215(Pt 8):1373-83. (PMID: 22442376)
J Nutr. 2006 Dec;136(12):2965-9. (PMID: 17116704)
J Anim Sci. 2010 May;88(5):1759-70. (PMID: 20081079)
J Exp Biol. 2012 Jan 1;215(Pt 1):169-78. (PMID: 22162865)
Aquat Toxicol. 2017 Jul;188:148-158. (PMID: 28527383)
Br J Nutr. 2014 Aug 28;112(4):493-503. (PMID: 24877663)
J Cell Mol Med. 2014 Apr;18(4):590-9. (PMID: 24834523)
Nucleic Acids Res. 2001 May 1;29(9):e45. (PMID: 11328886)
Br J Nutr. 2000 Nov;84(5):619-28. (PMID: 11177174)
J Lipid Res. 2007 Sep;48(9):1905-14. (PMID: 17699733)
BMC Genomics. 2010 Jan 17;11:39. (PMID: 20078893)
Comp Biochem Physiol A Mol Integr Physiol. 2011 Apr;158(4):391-9. (PMID: 21130894)
Int J Obes Relat Metab Disord. 2003 Oct;27(10):1178-86. (PMID: 14513065)
Front Endocrinol (Lausanne). 2016 Aug 22;7:113. (PMID: 27597840)
J Endocrinol. 2008 Sep;198(3):459-69. (PMID: 18579724)
Prostaglandins Leukot Essent Fatty Acids. 2013 Oct;89(5):359-66. (PMID: 24012207)
Clin Nutr. 2004 Aug;23(4):447-56. (PMID: 15297079)
Gen Comp Endocrinol. 2014 Sep 1;205:218-25. (PMID: 24846393)
Comp Biochem Physiol A Mol Integr Physiol. 2013 Jun;165(2):288-94. (PMID: 23542747)
Front Endocrinol (Lausanne). 2016 Apr 13;7:30. (PMID: 27148161)
Br J Nutr. 2011 Sep;106(5):633-47. (PMID: 21535902)
Prostaglandins Leukot Essent Fatty Acids. 2014 Jan;90(1):13-21. (PMID: 24332315)

AGL2014-57974-R; AGL2017-89436-R; BES-2015-074654; BES-2012-061867 Ministerio de Ciencia, Innovación y Universidades; 2017SGR-1574 Generalitat de Catalunya

Keywords: adipocyte; adipose tissue; eicosapentaenoic acid; linoleic acid; liver

0 (CD36 Antigens)
0 (Fatty Acid Transport Proteins)
0 (Fatty Acids)
0 (Peroxisome Proliferator-Activated Receptors)
0RBV727H71 (alpha-Linolenic Acid)
25167-62-8 (Docosahexaenoic Acids)
AAN7QOV9EA (Eicosapentaenoic Acid)
EC 2.3.1.85 (Fatty Acid Synthases)

Date Created: 20200304 Date Completed: 20201224 Latest Revision: 20201224

20250114

PMC7084833

10.3390/ijms21051623

32120851