Nanoplastics Cause Neurobehavioral Impairments, Reproductive and Oxidative Damages, and Biomarker Responses in Zebrafish: Throwing up Alarms of Wide Spread Health Risk of Exposure.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Biomarkers/*metabolism ; Nanoparticles/*toxicity ; Oxidative Stress/*drug effects ; Polystyrenes/*toxicity ; Water Pollution/*adverse effects ; Zebrafish/*metabolism; Aggression/drug effects ; Animals ; Behavior Rating Scale ; Behavior, Animal/drug effects ; Brain/diagnostic imaging ; Brain/drug effects ; Brain/metabolism ; Circadian Rhythm/drug effects ; Ecosystem ; Energy Metabolism/drug effects ; Gonads/diagnostic imaging ; Gonads/drug effects ; Gonads/metabolism ; Intestines/diagnostic imaging ; Intestines/drug effects ; Lipid Metabolism/drug effects ; Liver/diagnostic imaging ; Liver/drug effects ; Liver/metabolism ; Microscopy, Electron, Transmission ; Muscles/drug effects ; Muscles/metabolism ; Nanoparticles/chemistry ; Nanoparticles/ultrastructure ; Neurotransmitter Agents/metabolism ; Polystyrenes/chemistry ; Risk Assessment ; Spectrometry, Fluorescence ; Tissue Distribution/drug effects

Plastic pollution is a growing global emergency and it could serve as a geological indicator of the Anthropocene era. Microplastics are potentially more hazardous than macroplastics, as the former can permeate biological membranes. The toxicity of microplastic exposure on humans and aquatic organisms has been documented, but the toxicity and behavioral changes of nanoplastics (NPs) in mammals are scarce. In spite of their small size, nanoplastics have an enormous surface area, which bears the potential to bind even bigger amounts of toxic compounds in comparison to microplastics. Here, we used polystyrene nanoplastics (PS-NPs) (diameter size at ~70 nm) to investigate the neurobehavioral alterations, tissue distribution, accumulation, and specific health risk of nanoplastics in adult zebrafish. The results demonstrated that PS-NPs accumulated in gonads, intestine, liver, and brain with a tissue distribution pattern that was greatly dependent on the size and shape of the NPs particle. Importantly, an analysis of multiple behavior endpoints and different biochemical biomarkers evidenced that PS-NPs exposure induced disturbance of lipid and energy metabolism as well as oxidative stress and tissue accumulation. Pronounced behavior alterations in their locomotion activity, aggressiveness, shoal formation, and predator avoidance behavior were exhibited by the high concentration of the PS-NPs group, along with the dysregulated circadian rhythm locomotion activity after its chronic exposure. Moreover, several important neurotransmitter biomarkers for neurotoxicity investigation were significantly altered after one week of PS-NPs exposure and these significant changes may indicate the potential toxicity from PS-NPs exposure. In addition, after ~1-month incubation, the fluorescence spectroscopy results revealed the accumulation and distribution of PS-NPs across zebrafish tissues, especially in gonads, which would possibly further affect fish reproductive function. Overall, our results provided new evidence for the adverse consequences of PS-NPs-induced behavioral dysregulation and changes at the molecular level that eventually reduce the survival fitness of zebrafish in the ecosystem.

J Neuroendocrinol. 2008 Jun;20(6):858-65. (PMID: 18601710)
Biol Open. 2019 Jun 26;8(6):. (PMID: 31182629)
Behav Brain Res. 2013 Apr 15;243:294-9. (PMID: 23357085)
Water Res. 2019 May 15;155:410-422. (PMID: 30861380)
Environ Sci Technol. 2014;48(1):781-90. (PMID: 24295030)
Mar Pollut Bull. 2017 Nov 30;124(2):614-623. (PMID: 28012735)
Environ Pollut. 2015 Mar;198:211-22. (PMID: 25637744)
Sci Rep. 2013 Nov 21;3:3263. (PMID: 24263561)
Mar Pollut Bull. 2017 Sep 15;122(1-2):379-391. (PMID: 28684108)
Chemosphere. 2018 Sep;207:320-328. (PMID: 29803881)
J Neurosci Methods. 2012 Sep 30;210(2):266-71. (PMID: 22884772)
PLoS One. 2013 Apr 25;8(4):e62776. (PMID: 23638144)
PLoS One. 2012;7(11):e46751. (PMID: 23144787)
Toxicol Appl Pharmacol. 2010 Jan 15;242(2):224-30. (PMID: 19874833)
Soc Neurosci. 2009;4(3):276-86. (PMID: 19274578)
Mar Pollut Bull. 2004 May;48(9-10):835-9. (PMID: 15111030)
Arch Environ Contam Toxicol. 2015 Oct;69(3):279-87. (PMID: 26254899)
Mar Pollut Bull. 2014 Apr 15;81(1):55-60. (PMID: 24613232)
Mar Pollut Bull. 2014 Feb 15;79(1-2):94-9. (PMID: 24398418)
Water Res. 2015 May 15;75:63-82. (PMID: 25746963)
Ecotoxicol Environ Saf. 2010 Jan;73(1):101-7. (PMID: 19481804)
Front Endocrinol (Lausanne). 2016 Mar 31;7:28. (PMID: 27065948)
Biol Reprod. 2004 Nov;71(5):1712-23. (PMID: 15269096)
Environ Sci Technol. 2014;48(3):1638-45. (PMID: 24341789)
Environ Pollut. 2007 Sep;149(1):18-24. (PMID: 17360091)
Neuropeptides. 2002 Apr-Jun;36(2-3):221-9. (PMID: 12359512)
Sci Rep. 2016 Sep 27;6:34182. (PMID: 27669995)
Nihon Shinkei Seishin Yakurigaku Zasshi. 2002 Apr;22(2):55-9. (PMID: 12050853)
Oncotarget. 2017 Jul 7;8(65):109799-109817. (PMID: 29312650)
Toxicol Lett. 2005 Aug 14;158(2):122-32. (PMID: 16039401)
Brain Sci. 2012 Dec;2(4):. (PMID: 24349762)
J Biomed Mater Res A. 2004 Feb 1;68(2):244-56. (PMID: 14704966)
Environ Int. 2017 May;102:165-176. (PMID: 28284818)
Mar Pollut Bull. 2015 Sep 15;98(1-2):179-87. (PMID: 26456303)
PLoS One. 2012;7(5):e36931. (PMID: 22615849)
Sci Rep. 2017 Sep 7;7(1):10772. (PMID: 28883417)
Physiol Behav. 2011 Mar 1;102(3-4):296-303. (PMID: 21112347)
Environ Pollut. 2016 Jul;214:177-184. (PMID: 27086073)
Behav Genet. 2013 Mar;43(2):161-7. (PMID: 23354973)
PLoS One. 2012;7(2):e32254. (PMID: 22384193)
Neuroreport. 2003 Feb 10;14(2):233-8. (PMID: 12598736)
Psychopharmacology (Berl). 2002 Oct;163(3-4):434-58. (PMID: 12373445)
Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3573-8. (PMID: 23401542)
Nat Methods. 2014 Jul;11(7):743-8. (PMID: 24880877)
J Neurochem. 2003 Oct;87(1):195-204. (PMID: 12969266)
Med Sci Monit. 2005 Apr;11(4):RA136-45. (PMID: 15795710)
Comp Biochem Physiol C Toxicol Pharmacol. 2017 Sep;199:59-68. (PMID: 28274763)
Dev Psychobiol. 2018 Jan;60(1):43-56. (PMID: 29091281)
Environ Sci Technol. 2014;48(15):8823-30. (PMID: 24972075)
Sci Rep. 2017 Jul 14;7(1):5473. (PMID: 28710445)
Analyst. 2015 Apr 21;140(8):2687-95. (PMID: 25745670)
Eur J Pharmacol. 2005 Dec 5;526(1-3):51-64. (PMID: 16325649)
J Neurosci. 2001 May 1;21(9):3261-70. (PMID: 11312311)
Environ Pollut. 2016 May;212:251-256. (PMID: 26851981)
Int J Nanomedicine. 2018 Dec 11;13:8487-8506. (PMID: 30587973)
Am J Physiol Regul Integr Comp Physiol. 2009 Mar;296(3):R824-30. (PMID: 19052319)
Int J Clin Pract. 2007 May;61(5):835-45. (PMID: 17298593)
Nat Ecol Evol. 2017 Apr 20;1(5):116. (PMID: 28812686)
Amino Acids. 2012 Nov;43(5):2059-72. (PMID: 22491827)
Nat Protoc. 2010 Nov;5(11):1786-99. (PMID: 21030954)
Neurosci Res. 2007 May;58(1):32-9. (PMID: 17298851)
J Comp Neurol. 2009 Oct 1;516(4):321-33. (PMID: 19637308)
Sci Total Environ. 2017 Apr 15;584-585:1022-1031. (PMID: 28185727)
Proc Natl Acad Sci U S A. 2014 Mar 11;111(10):3841-6. (PMID: 24567386)
Water Res. 2016 Dec 1;106:364-370. (PMID: 27750125)
Horm Behav. 2018 Nov;106:35-43. (PMID: 30201533)
Mol Psychiatry. 2003 Aug;8(8):721-37, 715. (PMID: 12888801)
Comp Hepatol. 2003 Mar 6;2(1):4. (PMID: 12685931)
Environ Sci Technol. 2012 Oct 16;46(20):11327-35. (PMID: 22963286)
Proc Natl Acad Sci U S A. 2016 Mar 1;113(9):2430-5. (PMID: 26831072)
Food Chem Toxicol. 2013 Oct;60:177-80. (PMID: 23871786)
Endocrinology. 1998 Mar;139(3):905-12. (PMID: 9492019)
Horm Behav. 2012 Mar;61(3):227-9. (PMID: 22443808)
Endocr Rev. 1994 Dec;15(6):707-24. (PMID: 7705278)
Aquat Toxicol. 2014 Apr;149:1-7. (PMID: 24549118)
Behav Brain Res. 2011 Jan 1;216(1):166-71. (PMID: 20674614)
Environ Sci Technol. 2013 Jun 18;47(12):6646-55. (PMID: 23692270)
Sci Adv. 2017 Jul 19;3(7):e1700782. (PMID: 28776036)
Aquat Toxicol. 2000 Apr 1;48(4):549-559. (PMID: 10794836)
Environ Sci Technol. 2015 Jan 20;49(2):1130-7. (PMID: 25563688)
J Neurosci. 1991 Jul;11(7):2032-8. (PMID: 1648603)
Aquat Toxicol. 2016 Jan;170:89-103. (PMID: 26642093)
Sleep Med Rev. 2017 Aug;34:10-22. (PMID: 28648359)
Science. 2014 Jul 11;345(6193):144-5. (PMID: 25013051)
Inhal Toxicol. 2004 Jun;16(6-7):437-45. (PMID: 15204759)
Annu Rev Med. 2009;60:355-66. (PMID: 19630576)
Environ Sci Technol. 2014 Aug 19;48(16):9014-21. (PMID: 25059595)
Reprod Toxicol. 2015 Nov;57:165-75. (PMID: 26193689)
Nucleic Acids Res. 2012 Sep;40(16):7622-32. (PMID: 22661584)
Curr Biol. 2013 Dec 2;23(23):2388-92. (PMID: 24309271)
J Neurosci. 2005 Sep 21;25(38):8725-34. (PMID: 16177042)
Brain Res. 2001 Jun 8;903(1-2):263-8. (PMID: 11382414)
Horm Behav. 2002 Aug;42(1):53-61. (PMID: 12191647)
Environ Health Perspect. 1995 Oct;103 Suppl 7:173-8. (PMID: 8593867)
PLoS Biol. 2005 Nov;3(11):e351. (PMID: 16176122)
Environ Sci Technol. 2017 Nov 21;51(22):13397-13406. (PMID: 29059522)
Annu Rev Physiol. 2001;63:647-76. (PMID: 11181971)
Behav Brain Res. 2008 Mar 17;188(1):168-77. (PMID: 18054804)
Biol Psychiatry. 2003 Nov 15;54(10):960-71. (PMID: 14625138)

MOST 105-2313-B-033-001-MY3; MOST 107-2622-B-033-001-CC2 Ministry of Science and Technology

Keywords: behavior test; ecotoxicity; nanoplastics; neurotoxicity; oxidative stress; polystyrene; zebrafish

0 (Biomarkers)
0 (Neurotransmitter Agents)
0 (Polystyrenes)

Date Created: 20200226 Date Completed: 20210104 Latest Revision: 20210104

20231215

PMC7073134

10.3390/ijms21041410

32093039