NarL, a Novel Repressor for CYP108j1 Expression during PAHs Degradation in Rhodococcus sp. P14.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Gene Expression Regulation, Bacterial* ; Transcription, Genetic*; Bacterial Proteins/*metabolism ; Cytochrome P-450 Enzyme System/*metabolism ; Polycyclic Aromatic Hydrocarbons/*metabolism ; Repressor Proteins/*metabolism ; Rhodococcus/*metabolism; Bacterial Proteins/genetics ; Base Sequence ; Cytochrome P-450 Enzyme System/genetics ; Operon ; Promoter Regions, Genetic ; Repressor Proteins/genetics ; Rhodococcus/genetics ; Rhodococcus/growth & development

Rhodococcus sp. P14 was isolated from crude-oil-contaminated sediments, and a wide range of polycyclic aromatic hydrocarbons (PAHs) could be used as the sole source of carbon and energy. A key CYP450 gene, designated as cyp108j1 and involved in the degradation of PAHs, was identified and was able to hydroxylate various PAHs. However, the regulatory mechanism of the expression of cyp108j1 remains unknown. In this study, we found that the expression of cyp108j1 is negatively regulated by a LuxR (helix-turn-helix transcription factors in acyl-homoserine lactones-mediated quorum sensing) family regulator, NarL (nitrate-dependent two-component regulatory factor), which is located upstream of cyp108j1 . Further analysis revealed that NarL can directly bind to the promoter region of cyp108j1 . Mutational experiments demonstrated that the binding site between NarL and the cyp108j1 promoter was the palindromic sequence GAAAGTTG-CAACTTTC. Together, the finding reveal that NarL is a novel repressor for the expression of cyp108j1 during PAHs degradation.

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31670117 National Natural Science Foundation of China; 31700109 National Natural Science Foundation of China

Keywords: LuxR; PAHs; Rhodococcus; palindromic sequences; repressor

0 (Bacterial Proteins)
0 (Polycyclic Aromatic Hydrocarbons)
0 (Repressor Proteins)
9035-51-2 (Cytochrome P-450 Enzyme System)

Date Created: 20200207 Date Completed: 20201117 Latest Revision: 20201117

20240829

PMC7037279

10.3390/ijms21030983

32024188