A Comparison of Osteoblast and Osteoclast In Vitro Co-Culture Models and Their Translation for Preclinical Drug Testing Applications.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Drug Evaluation, Preclinical*; Bone Density Conservation Agents/*pharmacology ; Bone Remodeling/*drug effects ; Osteoblasts/*cytology ; Osteoclasts/*cytology ; Osteoporosis/*drug therapy; Animals ; Coculture Techniques ; Humans ; Osteoblasts/drug effects ; Osteoclasts/drug effects

As the population of western societies on average ages, the number of people affected by bone remodeling-associated diseases such as osteoporosis continues to increase. The development of new therapeutics is hampered by the high failure rates of drug candidates during clinical testing, which is in part due to the poor predictive character of animal models during preclinical drug testing. Co-culture models of osteoblasts and osteoclasts offer an alternative to animal testing and are considered to have the potential to improve drug development processes in the future. However, a robust, scalable, and reproducible 3D model combining osteoblasts and osteoclasts for preclinical drug testing purposes has not been developed to date. Here we review various types of osteoblast-osteoclast co-culture models and outline the remaining obstacles that must be overcome for their successful translation.

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Keywords: co-culture; drug testing; in vitro model; osteoblast; osteoclast; osteoporosis

0 (Bone Density Conservation Agents)

Date Created: 20200206 Date Completed: 20201117 Latest Revision: 20231113

20231113

PMC7037207

10.3390/ijms21030912

32019244