Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

High-throughput phenotypic screening of random genomic fragments in transgenic rice identified novel drought tolerance genes.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: Springer Country of Publication: Germany NLM ID: 0145600 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-2242 (Electronic) Linking ISSN: 00405752 NLM ISO Abbreviation: Theor Appl Genet Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Berlin, New York, Springer
    • الموضوع:
      Droughts* ; Genes, Plant* ; High-Throughput Screening Assays*; Adaptation, Physiological/*genetics ; Oryza/*genetics ; Oryza/*physiology; Amino Acid Sequence ; Gene Library ; Peptides/chemistry ; Phenotype ; Plants, Genetically Modified ; Reproducibility of Results ; Transcription, Genetic
    • نبذة مختصرة :
      Key Message: Novel drought tolerance genes were identified by screening thousands of random genomic fragments from grass species in transgenic rice. Identification of agronomically important genes is a critical step for crop breeding through biotechnology. Multiple approaches have been employed to identify new gene targets, including comprehensive screening platforms for gene discovery such as the over-expression of libraries of cDNA clones. In this study, random genomic fragments from plants were introduced into rice and screened for drought tolerance in a high-throughput manner with the aim of finding novel genetic elements not exclusively limited to coding sequences. To illustrate the power of this approach, genomic libraries were constructed from four grass species, and screening a total of 50,825 transgenic rice lines for drought tolerance resulted in the identification of 12 reproducibly efficacious fragments. Of the twelve, two were from the mitochondrial genome of signal grass and ten were from the nuclear genome of buffalo grass. Subsequent sequencing and analyses revealed that the ten fragments from buffalo grass carried a similar genetic element with no significant homology to any previously characterized gene. The deduced protein sequence was rich in acidic amino acid residues in the C-terminal half, and two of the glutamic acid residues in the C-terminal half were shown to play an important role in drought tolerance. The results demonstrate that an open-ended screening approach using random genomic fragments could discover trait genes distinct from gene discovery based on known pathways or biased toward coding sequence over-expression.
    • References:
      Nature. 2000 Dec 14;408(6814):796-815. (PMID: 11130711)
      Mol Cell Biol. 1997 Jan;17(1):115-22. (PMID: 8972191)
      Nat Biotechnol. 2015 Aug;33(8):862-9. (PMID: 26473199)
      Plant J. 1994 Aug;6(2):271-82. (PMID: 7920717)
      Plant Sci. 2018 Aug;273:110-119. (PMID: 29907303)
      Proc Natl Acad Sci U S A. 2000 Feb 15;97(4):1908-13. (PMID: 10660679)
      EMBO J. 1990 Oct;9(10):3353-62. (PMID: 1698615)
      Nat Plants. 2017 Jul 31;3:17107. (PMID: 28758991)
      Methods Mol Biol. 2014;1099:219-34. (PMID: 24243207)
      Methods Mol Biol. 2015;1223:189-98. (PMID: 25300841)
      Plant Mol Biol. 2007 Nov;65(4):357-71. (PMID: 17929174)
      Mutat Res Rev Mutat Res. 2015 Jul-Sep;765:19-39. (PMID: 26281766)
      Genome Res. 2014 May;24(5):831-8. (PMID: 24518071)
      Eur J Biochem. 1999 Jun;262(2):247-57. (PMID: 10336605)
      Nature. 2005 Aug 11;436(7052):793-800. (PMID: 16100779)
      Genetics. 1996 Aug;143(4):1843-60. (PMID: 8844169)
      J Hered. 2018 Feb 14;109(2):103-116. (PMID: 28992310)
      Plant Physiol. 2001 Nov;127(3):1136-46. (PMID: 11706193)
      Science. 2009 Nov 20;326(5956):1112-5. (PMID: 19965430)
      Plant Biotechnol J. 2014 Aug;12(6):685-93. (PMID: 24618117)
      Nat Protoc. 2008;3(5):824-34. (PMID: 18451790)
      Plant J. 2000 Jul;23(2):255-65. (PMID: 10929119)
      Mol Biosyst. 2015 Aug;11(8):2144-51. (PMID: 26088262)
      Plant J. 2009 Mar;57(5):883-94. (PMID: 18980645)
      Science. 1990 Nov 16;250(4983):959-66. (PMID: 17746920)
      Plasmid. 1992 Mar;27(2):161-3. (PMID: 1319594)
      Nat Biotechnol. 1996 Jun;14(6):745-50. (PMID: 9630983)
      Plant Mol Biol. 1992 Feb;18(4):675-89. (PMID: 1313711)
      Plant Cell Physiol. 2015 Nov;56(11):2248-58. (PMID: 26454879)
      Plant J. 2006 Dec;48(6):974-85. (PMID: 17227551)
      Cell. 2006 Dec 29;127(7):1309-21. (PMID: 17190597)
      J Mol Biol. 1990 Oct 5;215(3):403-10. (PMID: 2231712)
    • الرقم المعرف:
      0 (Peptides)
    • الموضوع:
      Date Created: 20200126 Date Completed: 20201110 Latest Revision: 20220413
    • الموضوع:
      20240628
    • الرقم المعرف:
      10.1007/s00122-020-03548-6
    • الرقم المعرف:
      31980835