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Platelet Adhesion and Thrombus Formation in Microchannels: The Effect of Assay-Dependent Variables.

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  • معلومة اضافية
    • المصدر:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Basel, Switzerland : MDPI, [2000-
    • الموضوع:
      Adenosine Monophosphate/*analogs & derivatives ; Aspirin/*pharmacology ; Microfluidics/*instrumentation ; Platelet Adhesiveness/*drug effects ; Platelet Aggregation/*drug effects ; Platelet Aggregation Inhibitors/*pharmacology ; Thrombosis/*etiology; Adenosine Monophosphate/pharmacology ; Blood Platelets/drug effects ; Collagen ; Cyclooxygenase Inhibitors/pharmacology ; Humans ; Male ; Purinergic P2Y Receptor Antagonists/pharmacology ; Thrombosis/diagnostic imaging ; Thrombosis/metabolism ; Young Adult
    • نبذة مختصرة :
      Microfluidic flow chambers (MFCs) allow the study of platelet adhesion and thrombus formation under flow, which may be influenced by several variables. We developed a new MFC, with which we tested the effects of different variables on the results of platelet deposition and thrombus formation on a collagen-coated surface.
      Methods: Whole blood was perfused in the MFC over collagen Type I for 4 min at different wall shear rates (WSR) and different concentrations of collagen-coating solutions, keeping blood samples at room temperature or 37 °C before starting the experiments. In addition, we tested the effects of the antiplatelet agent acetylsalicylic acid (ASA) (antagonist of cyclooxygenase-1, 100 µM) and cangrelor (antagonist of P2Y 12 , 1 µM).
      Results: Platelet deposition on collagen (I) was not affected by the storage temperature of the blood before perfusion (room temperature vs. 37 °C); (II) was dependent on a shear rate in the range between 300/s and 1700/s; and (III) was influenced by the collagen concentration used to coat the microchannels up to a value of 10 µg/mL. ASA and cangrelor did not cause statistically significant inhibition of platelet accumulation, except for ASA at low collagen concentrations.
      Conclusions: Platelet deposition on collagen-coated surfaces is a shear-dependent process, not influenced by the collagen concentration beyond a value of 10 µg/mL. However, the inhibitory effect of antiplatelet drugs is better observed using low concentrations of collagen.
      Competing Interests: The authors declare no conflict of interest.
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    • Grant Information:
      Fellowship 2018 and 2019 Fondazione Umberto Veronesi
    • Contributed Indexing:
      Keywords: antiplatelet agents; flow assays; microfluidics; platelet adhesion; platelet aggregation; platelets; shear rate; thrombus formation
    • الرقم المعرف:
      0 (Cyclooxygenase Inhibitors)
      0 (Platelet Aggregation Inhibitors)
      0 (Purinergic P2Y Receptor Antagonists)
      415SHH325A (Adenosine Monophosphate)
      6AQ1Y404U7 (cangrelor)
      9007-34-5 (Collagen)
      R16CO5Y76E (Aspirin)
    • الموضوع:
      Date Created: 20200126 Date Completed: 20201113 Latest Revision: 20201113
    • الموضوع:
      20221213
    • الرقم المعرف:
      PMC7037340
    • الرقم المعرف:
      10.3390/ijms21030750
    • الرقم المعرف:
      31979370