Non-gradient and genotype-dependent patterns of RSV gene expression.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Transcription, Genetic*/drug effects; RNA, Viral/*metabolism ; Respiratory Syncytial Virus, Human/*genetics; Adenosine Monophosphate/analogs & derivatives ; Adenosine Monophosphate/pharmacology ; Alanine/analogs & derivatives ; Alanine/pharmacology ; Animals ; Cell Line ; Female ; Genotype ; Half-Life ; Humans ; Male ; RNA Stability ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA, Viral/genetics ; Respiratory Syncytial Virus Infections/pathology ; Respiratory Syncytial Virus Infections/virology ; Respiratory Syncytial Virus, Human/isolation & purification ; Sigmodontinae ; Virus Replication

Respiratory syncytial virus (RSV) is a nonsegmented negative-strand RNA virus (NSV) and a leading cause of severe lower respiratory tract illness in infants and the elderly. Transcription of the ten RSV genes proceeds sequentially from the 3' promoter and requires conserved gene start (GS) and gene end (GE) signals. Previous studies using the prototypical GA1 genotype Long and A2 strains have indicated a gradient of gene transcription extending across the genome, with the highest level of mRNA coming from the most promoter-proximal gene, the first nonstructural (NS1) gene, and mRNA levels from subsequent genes dropping until reaching a minimum at the most promoter-distal gene, the polymerase (L) gene. However, recent reports show non-gradient levels of mRNA, with higher than expected levels from the attachment (G) gene. It is unknown to what extent different transcript stabilities might shape measured mRNA levels. It is also unclear whether patterns of RSV gene expression vary, or show strain- or genotype-dependence. To address this, mRNA abundances from five RSV genes were measured by quantitative real-time PCR (qPCR) in three cell lines and in cotton rats infected with RSV isolates belonging to four genotypes (GA1, ON, GB1, BA). Relative mRNA levels reached steady-state between four and 24 hours post-infection. Steady-state patterns were non-gradient and genotype-specific, where mRNA levels from the G gene exceeded those from the more promoter-proximal nucleocapsid (N) gene across isolates. Transcript stabilities could not account for the non-gradient patterns observed, indicating that relative mRNA levels more strongly reflect transcription than decay. Our results indicate that gene expression from a small but diverse set of RSV genotypes is non-gradient and genotype-dependent. We propose novel models of RSV transcription that can account for non-gradient transcription.
Competing Interests: The authors have declared that no competing interests exist.

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0 (RNA, Messenger)
0 (RNA, Viral)
3QKI37EEHE (remdesivir)
415SHH325A (Adenosine Monophosphate)
OF5P57N2ZX (Alanine)

Date Created: 20200111 Date Completed: 20200409 Latest Revision: 20200409

20240628

PMC6953876

10.1371/journal.pone.0227558

31923213