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Ultrasensitive haptoglobin biomarker detection based on amplified chemiluminescence of magnetite nanoparticles.
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- معلومة اضافية
- المصدر:
Publisher: BioMed Central Country of Publication: England NLM ID: 101152208 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-3155 (Electronic) Linking ISSN: 14773155 NLM ISO Abbreviation: J Nanobiotechnology Subsets: MEDLINE
- بيانات النشر:
Original Publication: London : BioMed Central, 2003-
- الموضوع:
- نبذة مختصرة :
Background: Haptoglobin is an acute-phase protein used as predicting diagnostic biomarker both in humans (i.e., diabetes, ovarian cancer, some neurological and cardiovascular disorders) and in animals (e.g., bovine mastitis). The latter is a frequent disease of dairy industry with staggering economical losses upon decreased milk production and increased health care costs. Early stage diagnosis of the associated diseases or inflammation onset is almost impossible by conventional analytical manners.
Results: The present study demonstrates a simple, rapid, and cost-effective label-free chemiluminescence bioassay based on magnetite nanoparticles (MNPs) for sensitive detection of haptoglobin by employing the specific interaction of hemoglobin-modified MNPs. The resulting haptoglobin-hemoglobin complex inhibits the peroxidase-like activity of luminol/H 2 O 2 -hemoglobin-MNPs sensing scheme and reduces the chemiluminescence intensities correspondingly to the innate haptoglobin concentrations. Quantitative detection of bovine haptoglobin was obtained within the range of 1 pg mL -1 to 1 µg mL -1 , while presenting 0.89 pg mL -1 limit of detection. Moreover, the influence of causative pathogenic bacteria (i.e., Streptococcus dysgalactiae and Escherichia coli) and somatic cell counts (depicting healthy, sub-clinical and clinical mastitis) on the emitted chemiluminescence radiation were established. The presented bioassay quantitative performances correspond with a standardized assay kit in differentiating dissimilar milk qualities.
Conclusions: Overall, the main advantage of the presented sensing concept is the ability to detect haptoglobin, at clinically relevant concentrations within real milk samples for early bio-diagnostic detection of mastitis and hence adjusting the precise treatment, potentially initiating a positive influence on animals' individual health and hence on dairy farms economy.
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- Contributed Indexing:
Keywords: Chemiluminescence; Haptoglobin; Magnetic iron oxide nanoparticles; Mastitis; Pathogens
- الرقم المعرف:
0 (Biomarkers)
0 (Haptoglobins)
0 (Magnetite Nanoparticles)
- الموضوع:
Date Created: 20200109 Date Completed: 20201009 Latest Revision: 20201009
- الموضوع:
20221213
- الرقم المعرف:
PMC6945394
- الرقم المعرف:
10.1186/s12951-019-0569-9
- الرقم المعرف:
31910856
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