NAI2 and TSA1 Drive Differentiation of Constitutive and Inducible ER Body Formation in Brassicaceae.

Publisher: Oxford University Press Country of Publication: Japan NLM ID: 9430925 Publication Model: Print Cited Medium: Internet ISSN: 1471-9053 (Electronic) Linking ISSN: 00320781 NLM ISO Abbreviation: Plant Cell Physiol Subsets: MEDLINE

Publication: Tokyo : Oxford University Press
Original Publication: Kyoto, Japan : Japanese Society of Plant Physiologists,

Arabidopsis/*genetics ; Arabidopsis Proteins/*genetics ; Brassicaceae/*genetics ; Endoplasmic Reticulum/*genetics; Arabidopsis/metabolism ; Arabidopsis Proteins/metabolism ; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/genetics ; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/metabolism ; Brassicaceae/metabolism ; Calcium-Binding Proteins ; Cyclopentanes/pharmacology ; DNA, Plant/genetics ; DNA, Plant/isolation & purification ; Endoplasmic Reticulum/metabolism ; Gene Duplication ; Gene Expression Regulation, Plant ; Oxylipins/pharmacology ; Phylogeny ; Plant Leaves/metabolism ; Promoter Regions, Genetic ; Seedlings/genetics ; Seedlings/metabolism ; Trans-Activators/genetics ; Trans-Activators/metabolism

Brassicaceae and closely related species develop unique endoplasmic reticulum (ER)-derived structures called ER bodies, which accumulate β-glucosidases/myrosinases that are involved in chemical defense. There are two different types of ER bodies: ER bodies constitutively present in seedlings (cER bodies) and ER bodies in rosette leaves induced by treatment with the wounding hormone jasmonate (JA) (iER bodies). Here, we show that At-α whole-genome duplication (WGD) generated the paralogous genes NAI2 and TSA1, which consequently drive differentiation of cER bodies and iER bodies in Brassicaceae plants. In Arabidopsis, NAI2 is expressed in seedlings where cER bodies are formed, whereas TSA1 is expressed in JA-treated leaves where iER bodies are formed. We found that the expression of NAI2 in seedlings and the JA inducibility of TSA1 are conserved across other Brassicaceae plants. The accumulation of NAI2 transcripts in Arabidopsis seedlings is dependent on the transcription factor NAI1, whereas the JA induction of TSA1 in rosette leaves is dependent on MYC2, MYC3 and MYC4. We discovered regions of microsynteny, including the NAI2/TSA1 genes, but the promoter regions are differentiated between TSA1 and NAI2 genes in Brassicaceae. This suggests that the divergence of function between NAI2 and TSA1 occurred immediately after WGD in ancestral Brassicaceae plants to differentiate the formation of iER and cER bodies. Our findings indicate that At-α WGD enabled diversification of defense strategies, which may have contributed to the massive diversification of Brassicaceae plants.
(© The Author(s) 2019. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.)

Erratum in: Plant Cell Physiol. 2020 Apr 1;61(4):863. (PMID: 32289174)

Keywords: Brassicaceae; ER body; Endoplasmic reticulum; Jasmonic acid; Myc transcription factor; Whole-genome duplication

0 (Arabidopsis Proteins)
0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors)
0 (Calcium-Binding Proteins)
0 (Cyclopentanes)
0 (DNA, Plant)
0 (MYC2 protein, Arabidopsis)
0 (MYC3 protein, Arabidopsis)
0 (MYC4 protein, Arabidopsis)
0 (NAI2 protein, Arabidopsis)
0 (Oxylipins)
0 (TSK-associating protein 1, Arabidopsis)
0 (Trans-Activators)
6RI5N05OWW (jasmonic acid)

Date Created: 20191228 Date Completed: 20210104 Latest Revision: 20210104

20240628

10.1093/pcp/pcz236

31879762