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Forsythoside A and Forsythoside B Contribute to Shuanghuanglian Injection-Induced Pseudoallergic Reactions through the RhoA/ROCK Signaling Pathway.
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- معلومة اضافية
- المصدر:
Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
- بيانات النشر:
Original Publication: Basel, Switzerland : MDPI, [2000-
- الموضوع:
- نبذة مختصرة :
In recent years, hypersensitivity reactions to the Shuanghuanglian injection have attracted broad attention. However, the componential chief culprits inducing the reactions and the underlying mechanisms involved have not been completely defined. In this study, we used a combination of approaches based on the mouse model, human umbilical vein endothelial cell monolayer, real-time cellular monitoring, immunoblot analysis, pharmacological inhibition, and molecular docking. We demonstrated that forsythoside A and forsythoside B contributed to Shuanghuanglian injection-induced pseudoallergic reactions through activation of the RhoA/ROCK signaling pathway. Forsythoside A and forsythoside B could trigger dose-dependent vascular leakage in mice. Moreover, forsythoside A and forsythoside B slightly elicited mast cell degranulation. Correspondingly, treatment with forsythoside A and forsythoside B disrupted the endothelial barrier and augmented the expression of GTP-RhoA, p-MYPT1, and p-MLC2 in a concentration-dependent manner. Additionally, the ROCK inhibitor effectively alleviated forsythoside A/forsythoside B-induced hyperpermeability in both the endothelial cells and mice. Similar responses were not observed in the forsythoside E-treated animals and cells. These differences may be related to the potential of the tested compounds to react with RhoA-GTPγS and form stable interactions. This study innovatively revealed that some forsythosides may cause vascular leakage, and therefore, limiting their contents in injections should be considered.
- References:
Sci Rep. 2017 Sep 22;7(1):12174. (PMID: 28939897)
J Pharm Biomed Anal. 2015 Nov 10;115:86-106. (PMID: 26177215)
J Anal Methods Chem. 2014;2014:241505. (PMID: 24719777)
Molecules. 2017 Sep 04;22(9):null. (PMID: 28869577)
Med J Aust. 1998 Dec 7-21;169(11-12):579-82. (PMID: 9887898)
Phytother Res. 2011 Mar;25(3):338-42. (PMID: 20677175)
Cardiovasc Res. 2010 Jul 15;87(2):243-53. (PMID: 20299335)
FEBS Lett. 2018 Jun;592(11):1763-1776. (PMID: 29749605)
Nat Commun. 2015 Apr 10;6:6725. (PMID: 25857352)
Expert Rev Mol Med. 2009 Jun 30;11:e19. (PMID: 19563700)
J Ethnopharmacol. 2018 Jan 10;210:318-339. (PMID: 28887216)
Microb Pathog. 2018 Mar;116:158-163. (PMID: 29330061)
Comput Biol Chem. 2019 Dec;83:107109. (PMID: 31445419)
J Struct Funct Genomics. 2012 Dec;13(4):241-5. (PMID: 23001747)
J Evid Based Med. 2010 Feb;3(1):18-26. (PMID: 21349036)
J Cell Physiol. 2011 Feb;226(2):414-23. (PMID: 20672289)
Front Pharmacol. 2018 Feb 12;9:87. (PMID: 29487527)
Neurochem Res. 2016 Apr;41(4):659-65. (PMID: 26498935)
J Orthop Surg Res. 2018 Jun 5;13(1):139. (PMID: 29866149)
Evid Based Complement Alternat Med. 2013;2013:208467. (PMID: 23762111)
Molecules. 2018 Mar 06;23(3):null. (PMID: 29509714)
Int J Mol Med. 2014 Mar;33(3):661-9. (PMID: 24398620)
Sci Rep. 2018 Feb 23;8(1):3572. (PMID: 29476121)
Int Immunopharmacol. 2015 Sep;28(1):494-9. (PMID: 26209933)
Drug Discov Ther. 2011 Oct;5(5):211-9. (PMID: 22466368)
Acta Pharmacol Sin. 2010 Jul;31(7):849-54. (PMID: 20581858)
Cytotechnology. 2015 Mar;67(2):379-86. (PMID: 24443077)
J Ethnopharmacol. 2015 Jul 21;170:1-7. (PMID: 25978951)
Zhongguo Zhong Yao Za Zhi. 2011 Jul;36(14):1865-9. (PMID: 22016949)
Evid Based Complement Alternat Med. 2013;2013:987326. (PMID: 23606893)
Eur J Pharmacol. 2017 Oct 5;812:250-255. (PMID: 28733217)
PLoS One. 2017 Feb 17;12(2):e0172289. (PMID: 28212381)
Molecules. 2018 Dec 11;23(12):null. (PMID: 30544947)
Int J Mol Med. 2013 Dec;32(6):1273-80. (PMID: 24064570)
Sci Rep. 2016 Sep 13;6:33192. (PMID: 27619816)
Int Immunopharmacol. 2015 May;26(1):80-5. (PMID: 25797347)
Zhongguo Zhong Yao Za Zhi. 2015 Jul;40(14):2727-31. (PMID: 26666017)
Arch Dis Child. 1993 Apr;68(4):468-71. (PMID: 8503668)
Int Immunopharmacol. 2017 Feb;43:79-84. (PMID: 27984711)
Vascul Pharmacol. 2002 Nov;39(4-5):187-99. (PMID: 12747959)
Exp Ther Med. 2014 Jan;7(1):179-184. (PMID: 24348786)
Int J Mol Med. 2017 Jul;40(1):243-250. (PMID: 28534936)
Biomed Chromatogr. 2019 Feb;33(2):e4376. (PMID: 30168866)
Neurochem Int. 2019 May;125:57-66. (PMID: 30769029)
J Pharm Biomed Anal. 2014 Jan;88:602-8. (PMID: 24211723)
Int J Biol Sci. 2017 Jan 1;13(1):65-75. (PMID: 28123347)
- Contributed Indexing:
Keywords: RhoA/ROCK signaling pathway; Shuanghuanglian injection; forsythoside A; forsythoside B; forsythoside E; pseudo-allergic reactions
- الرقم المعرف:
0 (Caffeic Acids)
0 (Drugs, Chinese Herbal)
0 (Glucosides)
0 (Glycosides)
0 (forsythoside B)
0 (shuang-huang-lian)
124671-05-2 (RHOA protein, human)
EC 2.7.11.1 (rho-Associated Kinases)
EC 3.6.5.2 (rhoA GTP-Binding Protein)
OUH5BQ893P (forsythiaside)
- الموضوع:
Date Created: 20191218 Date Completed: 20200430 Latest Revision: 20200430
- الموضوع:
20221213
- الرقم المعرف:
PMC6940901
- الرقم المعرف:
10.3390/ijms20246266
- الرقم المعرف:
31842335
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