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Detection and characterization of IgE-binding factors (IgE-BF) within supernatants of the cell line RPMI-8866, normal human sera and sera from atopic patients.

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  • معلومة اضافية
    • المصدر:
      Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 0374672 Publication Model: Print Cited Medium: Print ISSN: 0019-2805 (Print) Linking ISSN: 00192805 NLM ISO Abbreviation: Immunology Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Oxford : Blackwell Scientific Publications
    • الموضوع:
      Prostatic Secretory Proteins*; Dermatitis, Atopic/*immunology ; Leukemia, B-Cell/*immunology ; Lymphokines/*analysis; Cell Line ; Chromatography, Gel ; Humans ; Kinetics ; Lymphokines/blood ; Lymphokines/metabolism ; Radioimmunoassay
    • نبذة مختصرة :
      IgE-binding factors (IgE-BF) have been shown to be important regulatory factors for IgE induction and suppression. The analysis of IgE-binding factor activity by a modified inhibition radioimmunoassay (RIA), as well as by monoclonal antibodies (mAb) was carried out in the supernatant of the Fc epsilon RII+ cell line RPMI-8866 as well as in human sera. Kinetics of IgE-BF showed optimal release from RPMI-8866 cells after 3-4 days. Gel filtration of the supernatant indicated binding activity at less than 100,000, 45,000 and 25,000 MW. Within normal human sera two peaks of IgE-BF activity were obtained at 45,000 and 25,000 MW. In sera with high IgE levels (atopic dermatitis) a peak at less than 100,000 was MW detected. Within this peak endogenous IgE was present. Addition of sodium dodecylsulphate induced a release of IgE-BF with a MW of 60,000.
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    • الرقم المعرف:
      0 (Lymphokines)
      0 (Prostatic Secretory Proteins)
      0 (beta-microseminoprotein)
      0 (immunoglobulin-binding factors)
    • الموضوع:
      Date Created: 19880901 Date Completed: 19881222 Latest Revision: 20181113
    • الموضوع:
      20221213
    • الرقم المعرف:
      PMC1385019
    • الرقم المعرف:
      3181994