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An impedimetric biosensor based on electrophoretically assembled ZnO nanorods and carboxylated graphene nanoflakes on an indium tin oxide electrode for detection of the DNA of Escherichia coli O157:H7.

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  • معلومة اضافية
    • المصدر:
      Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 7808782 Publication Model: Electronic Cited Medium: Internet ISSN: 1436-5073 (Electronic) Linking ISSN: 00263672 NLM ISO Abbreviation: Mikrochim Acta Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Wien ; New York : Springer-Verlag.
    • الموضوع:
      Biosensing Techniques/*methods ; DNA, Bacterial/*analysis ; Escherichia coli O157/*genetics ; Graphite/*chemistry ; Nanotubes/*chemistry ; Tin Compounds/*chemistry ; Zinc Oxide/*chemistry; Biosensing Techniques/instrumentation ; Carboxylic Acids/chemistry ; Electric Impedance ; Electrodes ; Electrophoresis ; Limit of Detection
    • نبذة مختصرة :
      Aminopropyltrimethoxysilane (APTMS)-functionalized zinc oxide (ZnO) nanorods and carboxylated graphene nanoflakes (c-GNF) were used in a composite that was electrophoretically deposited on an indium tin oxide (ITO) coated glass substrate. The modified ITO electrodes were characterized using various microscopic and spectroscopic techniques which confirm the deposition of the APTMS-ZnO/c-GNF composite. The electrodes have been used for the covalent immobilization of an Escherichia coli O157:H7 (E. coli)-specific DNA prob. Impedimetric studies revealed that the gene sensor displays linear response in a wide range of target DNA concentration (10 -16  M to 10 -6  M) with a detection limit of 0.1 fM. The studies on the cross-reactivity to other water-borne pathogens show that the bioelectrode is highly specific. Graphical abstractSchematic illustration for fabrication of nucleic acid biosensor for E. coli DNA detection using an ITO electrode modified with siloxane-functionalized zinc oxide (ZnO) nanorods and carboxylated graphene nanoflakes (c-GNFs).
    • References:
      Analyst. 2005 Apr;130(4):421-6. (PMID: 15846872)
      Nanoscale. 2013 Jun 7;5(11):4758-62. (PMID: 23604556)
      Chem Soc Rev. 2013 Jun 21;42(12):5425-38. (PMID: 23508125)
      Talanta. 2017 Jan 1;162:511-522. (PMID: 27837864)
      Talanta. 2011 Aug 15;85(2):943-9. (PMID: 21726722)
      Emerg Infect Dis. 2011 Jan;17(1):7-15. (PMID: 21192848)
      Anal Chim Acta. 2016 Sep 21;937:29-38. (PMID: 27590542)
      Chemphyschem. 2014 Jun 6;15(8):1550-61. (PMID: 24643996)
      Sensors (Basel). 2010;10(5):4855-86. (PMID: 22399911)
      Anal Chim Acta. 2007 Feb 19;584(2):268-74. (PMID: 17386614)
      ACS Appl Mater Interfaces. 2012 Mar;4(3):1770-6. (PMID: 22329919)
      Biosens Bioelectron. 2014 Sep 15;59:200-7. (PMID: 24727606)
      Nat Mater. 2009 Jul;8(7):543-57. (PMID: 19525947)
    • Grant Information:
      DST/INSPIRE/04/2015/000932 International DST
    • Contributed Indexing:
      Keywords: Biosensor; Electrophoretic deposition; Escherichia coli; Graphene nanoflakes; Zinc oxide
    • الرقم المعرف:
      0 (Carboxylic Acids)
      0 (DNA, Bacterial)
      0 (Tin Compounds)
      71243-84-0 (indium tin oxide)
      7782-42-5 (Graphite)
      SOI2LOH54Z (Zinc Oxide)
    • الموضوع:
      Date Created: 20191205 Date Completed: 20200511 Latest Revision: 20200511
    • الموضوع:
      20240829
    • الرقم المعرف:
      10.1007/s00604-019-3921-8
    • الرقم المعرف:
      31797052