Lactobacillus plantarum P2R3FA Isolated from Traditional Cereal-Based Fermented Food Increase Folate Status in Deficient Rats.

Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101521595 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6643 (Electronic) Linking ISSN: 20726643 NLM ISO Abbreviation: Nutrients Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI Publishing

Edible Grain/*microbiology ; Fermented Foods/*microbiology ; Folic Acid/*analysis ; Lactobacillales/*isolation & purification ; Lactobacillus plantarum/*isolation & purification; Animals ; Bread ; Ethiopia ; Folic Acid Deficiency ; Food Microbiology ; Male ; Rats ; Rats, Wistar

Folate deficiencies are widespread around the world. Promoting consumption of folate-rich foods could be a sustainable option to alleviate this problem. However, these foods are not always available. Cereals, being a staple food, could contribute to folate intake. They are fermented prior to consumption in many African countries, and fermentation can modify the folate content. In Ethiopia, injera is a widely consumed fermented flat bread. The main drivers of its fermentation are lactic acid bacteria (LAB). The aim of this work was to isolate and identify folate-producing LAB from injera fermented dough and to evaluate their ability to increase folate status after depletion in a rat model. Among the 162 strains isolated from 60 different fermentations, 19 were able to grow on a folate-free culture medium and produced 1 to 43 µg/L (24 h, 30 °C incubation). The four highest folate producers belonged to the Lactobacillus plantarum species. The most productive strain was able to enhance folate status after depletion in a rat model, despite the relatively low folate content of the feed supplemented with the strain. Folate-producing L. plantarum strain has potential use as a commercial starter in injera production.

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ERAfrica ERAFRICA IC-027, FP-226154 Seventh Framework Programme

Keywords: bioavailability; cereal; fermentation; folate; lactic acid bacteria; rats

935E97BOY8 (Folic Acid)

Date Created: 20191123 Date Completed: 20200625 Latest Revision: 20211204

20250114

PMC6893693

10.3390/nu11112819

31752138