Phosphorylation sites of microtubule-associated protein 1B (MAP 1B) are involved in axon growth and regeneration.

Publisher: BioMed Central Country of Publication: England NLM ID: 101468876 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-6606 (Electronic) Linking ISSN: 17566606 NLM ISO Abbreviation: Mol Brain Subsets: MEDLINE

Original Publication: London : BioMed Central

Axons/*metabolism ; Microtubule-Associated Proteins/*metabolism ; Nerve Regeneration/*physiology; Animals ; Antibodies/metabolism ; Brain/embryology ; Brain/metabolism ; Cells, Cultured ; Female ; Mice, Inbred C57BL ; Mice, Inbred ICR ; Microtubules/metabolism ; Phosphorylation ; Phosphoserine/metabolism ; Protein Binding ; Rats ; Sciatic Nerve/injuries ; Sciatic Nerve/pathology

The growth cone is a specialized structure that forms at the tip of extending axons in developing and regenerating neurons. This structure is essential for accurate synaptogenesis at developmental stages, and is also involved in plasticity-dependent synaptogenesis and axon regeneration in the mature brain. Thus, understanding the molecular mechanisms utilized by growth cones is indispensable to understanding neuronal network formation and rearrangement. Phosphorylation is the most important and commonly utilized protein modification in signal transduction. We previously identified microtubule-associated protein 1B (MAP 1B) as the most frequently phosphorylated protein among ~ 1200 phosphorylated proteins. MAP 1B has more than 10 phosphorylation sites that were present more than 50 times among these 1200 proteins. Here, we produced phospho-specific antibodies against phosphorylated serines at positions 25 and 1201 of MAP 1B that specifically recognize growing axons both in cultured neurons and in vivo in various regions of the embryonic brain. Following sciatic nerve injury, immunoreactivity with each antibody increased compared to the sham operated group. Experiments with transected and sutured nerves revealed that regenerating axons were specifically recognized by these antibodies. These results suggest that these MAP 1B phosphorylation sites are specifically involved in axon growth and that phospho-specific antibodies against MAP 1B are useful markers of growing/regenerating axons.

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Keywords: Axon regeneration; Development; Growth cone; MAP 1B; Phosphorylation

0 (Antibodies)
0 (Microtubule-Associated Proteins)
0 (microtubule-associated protein 1B)
17885-08-4 (Phosphoserine)

Date Created: 20191113 Date Completed: 20200608 Latest Revision: 20200608

20250114

PMC6849251

10.1186/s13041-019-0510-z

31711525